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Volumn 5, Issue 22, 2007, Pages 3657-3664

Enzyme-purification and catalytic transformations in a microstructured PASSflow reactor using a new tyrosine-based Ni-NTA linker system attached to a polyvinylpyrrolidinone-based matrix

Author keywords

[No Author keywords available]

Indexed keywords

CATALYTIC TRANSFORMATIONS; ENZYME-PURIFICATION;

EID: 35649028416     PISSN: 14770520     EISSN: None     Source Type: Journal    
DOI: 10.1039/b712804e     Document Type: Article
Times cited : (52)

References (42)
  • 28
    • 0005213409 scopus 로고    scopus 로고
    • For both linker systems we prepared five different polymeric samples (DVB/VBC/vinylpyrrolidinone vol %): sample 1 = 10: 50: 40; sample 2 = 5: 95: 0; sample 3 = 10/70/20; sample 4 = 20: 60: 20 and sample 5 = 5: 60: 35. Sample 3 gave the highest loading of tyrosine linker (30%) and protein immobilisation
    • S. Mallik I. Mallik Synlett 1996 734 736
    • (1996) Synlett , pp. 734-736
    • Mallik, S.1    Mallik, I.2
  • 35
    • 0014949207 scopus 로고
    • With a DMSO content of 0% no transformation was observed, while a DMSO content of 15% and higher led to reduced reaction rates at pH 7. For additional details on BAL refer to:
    • U. K. Laemmli Nature 1970 227 680 685
    • (1970) Nature , vol.227 , pp. 680-685
    • Laemmli, U.K.1
  • 36
    • 29744464071 scopus 로고    scopus 로고
    • Elution of the protein after termination of the reaction expectedly gave a positive Bradford assay Silylation and prevention of non-specific binding of proteins to the glass only had to be done once, even if several consecutive reactions were carried out in the PASSflow reactor
    • P. D. De Maria T. Stillger M. Pohl S. Wallert K.-H. Drauz H. Gröger H. Trautwein A. Liese J. Mol. Catal. B: Enzym. 2006 38 43 47
    • (2006) J. Mol. Catal. B: Enzym. , vol.38 , pp. 43-47
    • De Maria, P.D.1    Stillger, T.2    Pohl, M.3    Wallert, S.4    Drauz, K.-H.5    Gröger, H.6    Trautwein, H.7    Liese, A.8


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.