Measurement of mutant frequency in T-cell receptor (TCR) gene by flow cytometry after X-irradiation on EL-4 mice lymphoma cells

Journal of Toxicological Sciences

Volumn 32, Issue 4, 2007, Pages 377-386

  Kunugita, Naoki ^a   Mei, Nan ^a,b   Goncharova, Tatjana ^a   Norimura, Toshiyuki ^a  

^a UNIVERSITY OF OCCUPATIONAL AND ENVIRONMENTAL HEALTH   (Japan)

^b NATIONAL CENTER FOR TOXICOLOGICAL RESEARCH   (United States)

Author keywords

EL 4 cell; Flow cytometry; Radiation; Somatic mutation; T cell receptor gene

Indexed keywords

CD3 ANTIBODY; CD4 ANTIGEN; PHYCOERYTHRIN; T LYMPHOCYTE RECEPTOR;

ANIMAL CELL; ARTICLE; CELL SURVIVAL; COMPLEX FORMATION; CONTROLLED STUDY; CYTOKINESIS; FLOW CYTOMETRY; GENE FREQUENCY; GENE MUTATION; IONIZING RADIATION; LYMPHOMA CELL; MICRONUCLEUS; MOUSE; MUTAGENICITY; NONHUMAN; SOMATIC MUTATION; X IRRADIATION;

MURINAE; MUS;

EID: 35649011449     PISSN: 03881350     EISSN: 18803989     Source Type: Journal    
DOI: 10.2131/jts.32.377     Document Type: Article

References (26)

1. Akiyama, M., Umeki, S., Kusunoki, Y., Kyoizumi, S., Nakamura, N., Mori, T., Ishikawa, Y., Yamakido, M., Ohama, K., Kodama, T., Endo, K. and Cologne, J.B. (1995): Somatic-cell mutations as a possible predictor of cancer risk. Health Phys., 68, 643-649.
2. Akleyev, A.V., Kossenko, M.M., Silkina, L.A., Degteva, M.O., Yachmenyov, V.A., Awa, A., Akiyama, M., Veremeyeva, G.A., Vozilova, A.V., Kyozumi, S., Kozheurov, V.P. and Vyushkova, O.V. (1995): Health effects of radiation incidents in the southern Urals. Stem Cells, 13 (Suppl. 1), 58-68.
3. Clevers, H., Alarcon, B., Wileman, T. and Terhorst, C. (1988): The T cell receptor/CD3 complex: A dynamic protein ensemble. Annu. Rev. Immunol., 6, 629-662.
4. Fenech, M. and Morley, A.A. (1985): Measurement of micronuclei in lymphocytes. Mutat. Res., 147, 29-36.
5. Fenech, M., Chang, W.P., Kirsch-Volders, M., Holland, N., Bonassi, S. and Zeiger, E. (2003): HUMN project: Detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures. Mutat. Res., 534, 65-75.
6. Heddle, J.A. and Swiger, R.R. (1996): Risk estimation from somatic mutation assays. Mutat. Res., 365, 107-117.
7. Hirota, H., Kubota, M., Adachi, S., Okuda, A., Lin, Y.W., Bessho, R., Wakazono, Y., Matsubara, K., Kuwakado, K., Akiyama, Y. and Tsutsui, T. (1994): Somatic mutations at T-cell antigen receptor and glycophorin A loci in pediatric leukemia patients following chemotherapy: Comparison with HPRT locus mutation. Mutat. Res., 315, 95-103.
8. Igari, K., Igari, Y., Okazaki, R., Kato, F., Ootsuyama, A. and Norimura, T. (2006): The delayed manifestation of T-cell receptor (TCR) variants in X-irradiated mice depends on Trp53 status. Radiat. Res., 166, 55-60.
9. Ishioka, N., Umeki, S., Hirai, Y., Akiyama, M., Kodama, T., Ohama, K. and Kyoizumi, S. (1997): Stimulated rapid expression in vitro for early detection of in vivo T-cell receptor mutations induced by radiation exposure. Mutat. Res., 390, 269-282.
10. Iwamoto, K.S., Hirai, Y., Umeki, S., Kusunoki, Y., Kyoizumi, S. , Kodama, T. , Ohama, K. , Nakamura, N. and Akiyama, M. (1994): A positive correlation between T-cell-receptor mutant frequencies and dicentric chromosome frequencies in lymphocytes from radiotherapy patients. J. Radiat. Res. 35, 92-103.
11. Kato, F., Kakihara, H., Kunugita, N., Ootsuyama, A. and Norimura, T. (2002): Role of p53 gene in apoptotic repair of genotoxic tissue damage in mice. J. Radiat. Res. 43 (Suppl.), S209-212.
12. + variant T cell frequency by flow cytometry after X-irradiation on mice. J. Occup. Health, 38, 25-29.
13. Kusunoki, Y., Hayashi, T., Hirai, Y., Kushiro, J., Tatsumi, K., Kurihara, T., Zghal, M., Kamoun, M.R., Takebe, H., Jeffreys, A., Nakamura, N. and Akiyama, M. (1994): Increased rate of spontaneous mitotic recombination in T lymphocytes from a Bloom's syndrome patient using a flow-cytometric assay at HLA-A locus. Jpn. J. Cancer Res., 85, 610-618.
14. + T cells. J. Exp. Med., 171, 1981-1999.
15. Kyoizumi, S., Umeki, S., Akiyama, M., Hirai, Y., Kusunoki, Y., Nakamura, N., Endoh, K., Konishi, J., Sasaki, M.S., Mori, T., Fujita, S. and Cologne, J.B. (1992): Frequency of mutant T lymphocytes defective in the expression of the T-cell antigen receptor gene among radiation-exposed people. Mutat. Res., 265, 173-180.
16. Kyoizumi, S., Kusunoki, Y., Seyama, T., Hatamochi, A. and Goto, M. (1998): In vivo somatic mutations in Werner's syndrome. Hum. Genet., 103, 405-410.
17. Lantelme, E., Mantovani, S., Palermo, B., Campanelli, R., Granziero, L., Monafo, V. and Giachino, C. (2000): Increased frequency of RAG-expressing, CD4(+)CD3(low) peripheral T lymphocytes in patients with defective responses to DNA damage. Eur. J. Immunol., 30, 1520-1525.
18. Mei, N., Kunugita, N., Nomoto, S. and Norimura, T. (1996): Comparison of the frequency of T-cell receptor mutants and thioguanine resistance induced by X-rays and ethylnitrosourea in cultured human blood T-lymphocytes. Mutat. Res., 357, 191-197.
19. Saenko, A.S., Zamulaeva, I.A., Smirnova, S.G., Orlova, N.V., Selivanova, E.I., Saenko, V.A., Matveeva, N.P., Kaplan, M.A., Nugis, V.Y., Nadezhina, N.M. and Tsyb, A.F. (1998): Determination of somatic mutant frequencies at glycophor in A and T-cell receptor loci for biodosimetry of prolonged irradiation. Int. J. Radiat. Biol., 73, 613-618.
20. Saenko, A.S., Zamulaeva, I.A., Smirnova, S.G., Orlova, N.V., Selivanova, E.I., Matveeva, N.P., Kaplan, M.A., Nugis, V.Y., Nadezhina, N.M. and Tsyb, A.F. (2000): Determination of somatic mutant frequencies at glycophorin A and T-cell receptor loci for biodosimetry of acute and prolonged irradiation. Appl. Radiat. Isot., 52, 1145-1148.
21. Sawada, M., Kubota, M., Lin, Y.W., Watanabe, K., Koishi, S., Usami, I., Akiyama, Y., Matsumura, T. and Furusho, K. (1998a): Prospective study of mutant frequencies at the hprt and T-cell receptor gene loci in pediatric cancer patients during chemotherapy. Cancer Epidemiol. Biomarkers Prev., 7, 711-717.
22. Sawada, M., Kubota, M., Lin, Y.W., Watanabe, K., Koishi, S., Usami, I., Akiyama, Y., Matsumura, T. and Furusho, K. (1998b): Evaluation of mutant frequencies at the hprt and the T-cell receptor loci in pediatric cancer patients before treatment. Mutat. Res., 397, 337-343.
23. Suzuki, T., Kusunoki, Y., Tsuyama, N., Ohnishi, H., Seyama, T. and Kyoizumi, S. (2001): Elevated in vivo frequencies of mutant T cells with altered functional expression of the T-cell receptor or hypoxanthine phosphoribosyltransferase genes in p53-deficient mice. Mutat. Res., 483, 13-17.
24. Umeki, S., Kyoizumi, S., Kusunoki, Y., Nakamura, N., Sasaki, M., Mori, T., Ishikawa, Y., Cologne, J.B. and Akiyama, M. (1991): Flow cytometric measurements of somatic cel l mutations in Thorotrast patients. Jpn. J. Cancer Res., 82, 1349-1353.
25. Umeki, S., Suzuki, T., Kusunoki, Y., Seyama, T., Fujita, S. and Kyoizumi, S. (1997): Development of a mouse model for studying in vivo T-cell receptor mutations. Mutat. Res., 393, 37-46.
26. Zamulaeva, I.A., Smirnova, S.G., Orlova, N.V., Vereshchagina, O.A., Chekin, S., Smirnova, I.A., Krikunova, L.I., Parshin, V.S., Ivanov, V.K. and Saenko, A.S. (2006): Somatic mutagenesis at T-cell receptor locus in inhabitants of radiation polluted regions as a result of the Chernobyl disaster. Radiats Biol. Radioecol., 46, 307-314.