Optimized protocol for the large scale production of HIV pseudovirions by transient transfection of HEK293T cells with linear fully deacylated polyethylenimine

Journal of Virological Methods

Volumn 146, Issue 1-2, 2007, Pages 298-304

  Devitt, Gerard ^a   Thomas, Mini ^b,c   Klibanov, Alexander M ^b,c   Pfeiffer, Tanya ^a   Bosch, Valerie ^a  

^a GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^c Massachusetts Institute of Technology Cambridge   (United States)

Author keywords

DNA transfection; HIV; Polyethylenimine; Pseudovirions

Indexed keywords

CALCIUM PHOSPHATE; POLYETHYLENEIMINE; PROTEIN;

ARTICLE; CELL STRAIN HEK293; CONTAMINATION; CONTROLLED STUDY; EMBRYO; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; VACCINATION; VIRION;

AIDS VACCINES; CELL LINE; GENETIC VECTORS; HIV; HUMANS; PLASMIDS; POLYETHYLENEIMINE; TRANSFECTION; VIRION;

MAMMALIA;

EID: 35648966566     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2007.07.011     Document Type: Article

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