Degradation of Cry1Ac protein within transgenic Bacillus thuringiensis rice tissues under field and laboratory conditions

Environmental Entomology

Volumn 36, Issue 5, 2007, Pages 1275-1282

  Li, Yunhe ^a,b,c   Wu, Kongming ^a   Zhang, Yongjun ^a   Yuan, Guohui ^b  

^a INSTITUTE OF PLANT PROTECTION   (China)

^b HENAN AGRICULTURAL UNIVERSITY   (China)

^c AGROSCOPE RECKENHOLZ TÄNIKON RESEARCH STATION ART   (Switzerland)

Author keywords

Bacillus thuringiensis; Cry1Ac protein; Degradation; Rice plant; Soil

Indexed keywords

BACTERIAL PROTEIN; BACTERIAL TOXIN; ENDOTOXIN; HEMOLYSIN; INSECTICIDAL CRYSTAL PROTEIN, BACILLUS THURINGIENSIS; INSECTICIDE; PESTICIDE RESIDUE; UNCLASSIFIED DRUG;

BIODEGRADATION; ENVIRONMENTAL FATE; FIELD METHOD; IMMUNOASSAY; LABORATORY METHOD; PLOWING; PROTEIN; RICE; TRANSGENIC PLANT;

ARTICLE; CHEMISTRY; RICE; SOIL POLLUTANT; TRANSGENIC PLANT; WATER POLLUTANT;

BACTERIAL PROTEINS; BACTERIAL TOXINS; ENDOTOXINS; HEMOLYSIN PROTEINS; INSECTICIDES; ORYZA SATIVA; PESTICIDE RESIDUES; PLANTS, GENETICALLY MODIFIED; SOIL POLLUTANTS; WATER POLLUTANTS, CHEMICAL;

BACILLUS THURINGIENSIS; BACILLUS THURINGIENSIS SEROVAR KURSTAKI;

EID: 35648934052     PISSN: 0046225X     EISSN: None     Source Type: Journal    
DOI: 10.1603/0046-225X(2007)36[1275:DOCPWT]2.0.CO;2     Document Type: Article

References (44)

1. Chen, D. H., G. Y. Ye, C. Q. Yang, Y. Chen, and Y. K. Wu. 2005. The effect of high temperature on the insecticidal properties of Bt cotton. Environ. Exp. Bot. 53: 333-340.
2. Crecchio, C., and G. Stotzky. 1998. Insecticidal activity and biodegradation of the proteins from Bacillus thuringiensis subsp. kurstaki bound to humic acids from soil. Soil. Biol. Biochem. 30: 463-470.
3. Crecchio, C., and G. Stotzky. 2001. Biodegradation and insecticidal activity of the protein from Bacillus thuringiensis subsp. kurstaki bound on complexes of montmorillonite-humic acids-A1 hydroxypolymers. Soil. Biol. Biochem. 33: 573-581.
4. Dale, P. J., B. Clarke, and E.M.G. Fontes. 2002. Potential for the environmental impact of transgenic crops. Nat. Biotechnol. 20: 567-574.
5. Dubelman, S., B. R. Ayden, B. M. Bader, C. R. Brown, C. J. Jiang, and D. Vlachos. 2005. Cry1Ab protein does not persist in soil after 3 years of sustained Bt corn use. Environ. Entomol. 34: 915-921.
6. Flores, S., D. Saxema, and D. Stotzky. 2005. Transgenic Bt plants decompose less in soil than non-Bt plants. Soil Biol. Biochem. 37: 1073-1082.
7. Giovannetti, M., C. Sbrana, and A. Turrini. 2005. The impact of genetically modified crops on soil microbial communities. Riv. Biol. Biol. Forum. 98: 393-417.
8. Hails, R. S. 2000. Genetically modified plants - the debate continues. Trends Ecol. Evol. 15: 14-18.
9. Han, L. Z., K. M. Wu, Y. F. Peng, F. Wang, and Y. Y. Guo. 2006. Evaluation of transgenic rice containing Cry1Ac and CpTI genes against Chilo suppressalis and its intrapopulation variation in susceptibility to Cry1Ac protein. Environ. Entomol. 35: 1453-1459.
10. Head, G., J. B. Surber, J. A. Watson, J. W. Martin, and J. J. Duan. 2002. No detection of Cry1Ac protein in soil after multiple years of transgenic Bt cotton (Bollgard) use. Environ. Entomol. 31: 30-36.
11. Hopkins, D. W., and E. G. Gregorich. 2003. Detection and decay of the Bt endotoxin in soil from a field trial with genetically modified maize. Eur. J. Soil Sci. 54: 793-800.
12. Hopkins, D. W., E. A. Webster, J. A. Chudek, and C. Halpin. 2001. Decomposition in soil of tobacco plants with genetic modifications to lignin biosynthesis. Soil Biol. Biochem. 33: 1455-1462.
13. James, C. 2006. Global status of commercialized/bioengineered crops: 2006. international service for the acquisition of agri-biotech applications. Ithaca, NY.
14. Jouanin, L., M. Bonadé-Bottino, C. Girard, G. Morrot, and M. Giband. 1998. Transgenic plants for insect resistance. Plant Sci. 131: 1-11.
15. Koskella, J., and G. Stotzky. 1997. Microbial utilization of free and clay-bound insecticidal toxins from Bacillus thuringiensis and their retention of insecticidal activity after incubation with microbes. Appl. Environ. Microbiol. 63: 3561-3568.
16. Lewellyn, D., Y. Cousins, A. Mathews, L. Hartweck, and B. Lyon. 1994. Expression of Bacillus thuringiensis insecticidal protein genes in transgenic crop plants. Agric. Ecosys. Environ. 49: 85-93.
17. Losey, J. E., L. S. Rayor, and M. E. Carter. 1999. Transgenic pollen harms monarch larvae. Nature (Lond.) 399: 214.
18. Obrycki, J. J., J. E. Losey, O. R. Taylor, and L. C. H. Jesse. 2001. Transgenic insecticidal corn: beyond insecticidal toxicity to ecological complexity. Bioscience 51: 353-361.
19. Palm, C. J., D. L. Schaller, K. K. Donegan, and R. J. Seidler. 1996. Persistence in soil of transgenic plant produced Bacillus thuringiensis var. kurstaki Δ-endoprotein. Can. J. Microbiol. 42: 1258-1262.
20. Palm, C. J., K. Donegan, D. Harris, and R. J. Seidler. 1994. Quantification in soil of Bacillus thuringiensis var. kurstaki Δ-endotoxin from transgenic plants. Mol. Ecol. 3: 145-151.
21. Pray, C., D. M. Ma, J. K. Huang, and F. B. Qiao. 2001. Impact of Bt cotton in China. World Develop. 29: 813-825.
22. Ream, J. E., S. A. Berberich, S. R. Sims, G. J. Rogan, and R. L. Fuchs. 1992. In planta distribution and environmental fate of insect resistant cotton proteins. Plant Physiol. 99: 80.
23. Rissler, J., and M. Mellon. 1996. The ecological risk of engineered crops. MIT, Cambridge, MA.
24. Romeis. J., M. Meissle, and F. Bigler. 2006. Transgenic crops expressing Bacillus thuringiensis toxins and biological control. Nat. Biotechnol. 24: 63-71.
25. Saxena, D., and G. Stotzky. 2000. Insecticidal protein from Bacillus thuringiensis is released from roots of transgenic Bt corn in vitro and in situ. FEMS. Microbiol. Ecol. 33: 35-39.
26. Saxena, D., and G. Stotzky. 2001. Bacillus thuringiensis (Bt) protein released from root exudates and biomass of Bt corn has no apparent effect on earthworms, nematodes, protozoa, and fungi in soil. Soil Biol. Biochem. 33: 1225-1230.
27. Saxena, D., N. C. Stewart, I. Altosaar, Q. Shu, and G. Stotzky. 2004. Larvicidal Cry proteins from Bacillus thuringiensis are released in root exudates of transgenic B. thuringiensis corn, potato, and rice but not of B. thuringiensis canola, cotton, and tobacco. Plant Physiol. Biochem. 42: 111-120.
28. Saxena, D., S. Flores, and G. Stotzky. 1999. Insecticidal protein in root exudates from Bt corn. Nature (Lond.) 402: 480.
29. Saxena, D., S. Flores, and G. Stotzky. 2002a. Bt toxin is released in root exudates from 12 transgenic corn hybrids representing three transformation events. Soil Biol. Biochem. 34: 133-137.
30. Saxena, D., S. Flores, and G. Stotzky. 2002b. Vertical movement in soil of insecticidal Cry1Ab protein from Bacillus thuringiensis. Soil Biol. Biochem. 34: 111-120.
31. Shelton, A. M., J. Z. Zhao, and R. T. Roush. 2002. Economic, ecological, food safety, and social consequences of the deployment of Bt transgenic plants. Annu. Rev. Entomol. 47: 845-881.
32. Sims, S. R., and J. E. Ream. 1997. Soil inactivation of the Bacillus thuringiensis subsp. kurstaki CryIIA insecticidal protein within transgenic cotton tissue: laboratory microcosm and field studies. J. Agric. Food Chem. 45: 1502-1505.
33. Sims, S. R., and L. R. Holden. 1996. Insect bioassay for determining soil degradation of Bacillus thuringiensis ssp. kurstaki cryIA (b) protein in corn tissue. Environ. Entomol. 25: 659-664.
34. StatSoft. 2005. STATISTICA (data analysis software system), version 6. StatSoft, Tulsa, OK.
35. Stotzky, G. 2000. Persistence and biological activity in soil of insecticidal proteins from Bacillus thuringiensis and of bacterial DNA bound on calys and humic acids. J. Environ. Qual. 29: 691-705.
36. Tapp, H., and G. Stotzky. 1995. Dot blot enzyme-linked immunosorbent assay for monitoring the fate of insecticidal proteins from Bacillus thuringiensis in soil. Appl. Environ. Microbiol. 61: 602-609.
37. Tapp, H., and G. Stotzky. 1998. Persistence of the insecticidal protein from Bacillus thuringiensis subsp. kurstaki in soil. Soil Biol. Biochem. 30: 471-476.
38. Tapp, H., L. Calamai, and G. Stotzky. 1994. Adsorption and binding of the insecticidal proteins from Bacillus thuringiensis subsp. kurstaki and subsp. tenebrionis on clay minerals. Soil Boil. Biochem. 26: 663-679.
39. Venkateswerlu, G., and G. Stotzky. 1992. Binding of the proprotein and protein proteins of Bacillus thuringiensis subsp. kurstaki on clay minerals. Curr. Microbiol. 25: 225-233.
40. Wang, H. Y., Q. F. Ye, J. Gan, and L. C. Wu. 2007. Biodegradation of Cry1Ab protein from Bt transgenic rice in aerobic and flooded paddy soils. J. Agric. Food Chem. 55: 1900-1904.
41. Wang, H. Y., Q. F. Ye, W. Wang, L. C. Wu, and W. X. Wu. 2006. Cry1Ab protein from Bt transgenic rice does not residue in rhizosphere soil. Environ. Pollut. 143: 449-455.
42. Williamson, M. 1992. Environmental risks from the release of genetically modified organisms (GMOS) - the need for molecular ecology. Mol. Ecol. 1: 3-8.
43. Zhou, X. Y., Q. F. Huang, S. W. Chen, and Z. N. Yu. 2005. Adsorption of the insecticidal protein of Bacillus thuringiensis on montmorillonite, kaolinite, silica, goethite and red soil. Appl. Clay Sci. 30: 87-93.
44. Zwahlen, C., A. Hilbeck, P. Gugerli, and W. Nentwig. 2003. Degradation of the Cry1Ab protein within transgenic Bacillus thuringiensis corn tissue in the field. Mol. Ecol. 12: 765-775.