No detection of Cry1Ac protein in soil after multiple years of transgenic Bt cotton (Bollgard) use

Environmental Entomology

Volumn 31, Issue 1, 2002, Pages 30-36

  Head, Graham ^a   Surber, James B ^a   Watson, Jon A ^a   Martin, John W ^a   Duan, Jian J ^a  

^a MONSANTO COMPANY   (United States)

Author keywords

Bacillus thuringiensis; Cry1Ac protein; Environmental risk; Enzyme linked immunosorbent assays; Insect bioassay; Transgenic cotton

Indexed keywords

COTTON; ENVIRONMENTAL RISK; IMMUNOASSAY; PLANT RESIDUE; SOIL AMENDMENT; TRANSGENIC PLANT;

BACILLUS THURINGIENSIS; BACILLUS THURINGIENSIS SEROVAR BERLINER; GOSSYPIUM HIRSUTUM; HELIOTHIS; HELIOTHIS VIRESCENS; INSECTA; LEPIDOPTERA; NICOTIANA TABACUM; NOCTUIDAE;

EID: 0036488326     PISSN: 0046225X     EISSN: None     Source Type: Journal    
DOI: 10.1603/0046-225X-31.1.30     Document Type: Article

References (26)

1. Bolin, P. C, W. D. Hutchison, and D. W. Davis. 1996. Resistant hybrids and Bacillus thuringiensis for management of European corn borer (Lepidoptera: Pyralidae) in sweet corn. J. Econ. Entomol. 89: 82-91.
2. Crecchio, C., and G. Stotzky. 1998. Insecticidal activity and biodegradation of the toxin from Bacillus thuringiensis subsp. kurstaki bound to humic acids from soil. Soil Biol. Biochem. 30: 463-470.
3. Federici, B. 1998. Broadscale use of pest-killing plants to be true test. Calif. Agric. 52: 14-20.
4. Fuchs, R. L., S. C. MacIntosh, D. A. Dean, J. D. Greenplate, F. G. Perlak, J. C. Pershing, P. G. Marrone, and D. A. Fischhoff. 1990. Quantification of Bacillus thuringiensis insect control protein as expressed in transgenic plants. ACS Symp. Ser. 432: 105-113.
5. Hoffmann, M. P., R. G. Luttrell, L. T. Wilson, J. M. Smilanick, L. D. Malyj, J. Kiser, V. A. Hilder, and W. B. Barnes. 1992. Field evaluation of transgenic tobacco containing genes encoding Bacillus thuringiensis delta-endotoxin or cowpea trypsin inhibitor: efficiency against Helicoverpa zea (Lepidoptera: Noctuidae). J. Econ. Entomol. 26: 939-945.
6. Hofte, H., and H. R. Whitely. 1989. Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol. Rev. 53: 242-255.
7. King, E. G. Jr., and G. G. Hartley. 1992. Multiple-species insect rearing in support of research, pp. 159-172. In T. E. Anderson and N. C. Leppla [eds.], Advances in insect rearing for research and pest management. Westview, Boulder, CO.
8. Kollwyek, D., and K. Hamilton. 1999. Protein levels in insect-resistant cotton samples produced in 1998 US field trial. Monsanto Company Field Project MSL 16081. Monsanto, St. Louis, MO.
9. Lewellyn, D., Y. Cousins, A. Mathews, L. Hartweck, and B. Lyon, 1994. Expression of Bacillus thuringiensis insecticidal protein genes in transgenic crop plants. Agric. Ecosys. Environ. 49: 85-93.
10. MacIntosh, S. C., T. B. Stone, S. R. Sims, P. L. Hunst, J. T. Greenplate, P. G. Marrone, F. J. Perlak, D. A. Fischhoff, and R. L. Fuchs. 1990. Specificity and efficacy of purified Bacillus thuringiensis proteins against agronomically important insects. J. Invertebr. Pathol. 56: 258-266.
11. Mascarenhas, V. J., and R. G. Luttrell. 1997. Combined effect of sublethal exposure to cotton expressing the endotoxin protein of Bacillus thuringiensis and natural enemies on survival of bollworm (Lepidoptera: Noctuidae) larvae. Environ. Entomol. 26: 939-945.
12. Orr, D. B., and D. A. Landis. 1997. Oviposition of European corn borer (Lepidoptera: Pyralidae) and impact of natural enemy populations in transgenic versus isogenic corn. J. Econ. Entomol. 90: 905-909.
13. Palm, C. J., K. K. Donegan, D. Harris, and R. J. Siedler. 1994. Quantification in soil of Bacillus thuringiensis var. kurstaki δ-endotoxin from transgenic plants. Mol. Ecol. 3: 145-151.
14. Palm, C. J., K. K. Donegan, and R. J. Siedler. 1996. Persistence in soil of transgenic plant produced Bacillus thuringiensis var. kurstaki δ-endotoxin. Can. J. Microbiol. 42: 1258-1262.
15. Persley, G. J. 1996. Biotechnology and integrated pest management. CAB, Oxon, UK.
16. Ream, J. E., S. R. Sims, and J. N. Leach. 1994. Aerobic soil degradation of Bacillus thuringiensis var. kurstaki HD-73 protein bioactivity. Monsanto Company Laboratory Project MSL 13267. Monsanto, St. Louis, MO.
17. Reed, G. L., A. S. Jensen, J. Riebe, G. Head, and J. J. Duan. 2001. Transgenic Bt potato and conventional insecticides for Colorado potato beetle management: comparative efficacy and non-target impacts. Entomol. Exp. Appl. 100: 89-100.
18. SAS Institute. 1995. JMP Statistics and graphics guide, version 3. SAS Institute, Cary, NC.
19. SAS Institute. 2000. SAS Statistical software, Version 8. SAS Institute, Cary, NC.
20. Schuler, T. H., R. P. Potting, J. I. Denholm, and G. M. Poppy. 1999. Parasitoid behaviour and Bt plants. Nature (Lond.) 400: 825.
21. Saxena, D., S. Flores, and G. Stotzky. 1999. Transgenic plants: insecticidal toxin in root exudates from Bt corn. Nature (Lond.) 402: 480.
22. Sims, S. R, and L. R. Holden. 1996. Insect bioassay for determining soil degradation of Bacillus thuringiensis subsp. kurstaki Cry1A (b) protein in corn tissue. Environ. Entomol. 25: 659-664.
23. Tapp, H., and G. Stotzky. 1995. Insecticidal activity of the toxins from Bacillus thuringiensis subspecies kurstaki and tenebrionis adsorbed and bound on pure and sooil clays. Appl. Environ. Microbiol 61: 1786-1790.
24. Tapp, H. L. Calamai, and G. Stotzky. 1994. Adsorption and binding of the insecticidal proteins from Bacillus thuringiensis subsp. kurstaki and subsp. tenebrionis on clay minerals. Soil Biol. Biochem 26: 663-679.
25. Venkateswerlu, G., and G. Stotzky. 1992. Binding of the protoxin and toxin proteins of Bacillus thuringiensis subs. kurstaki on day minerals. Curr. Microbiol. 25: 225-233.
26. Williamson, E. 1992. Environmental risks from the release of genetically modified organisms (GMOS) - the need for molecular ecology. Mol. Ecol. 1: 3-8.