Double-Layered collagen gel hemisphere for cell invasion assay: Successful visualization and quantification of cell Invasion activity

Cell Communication and Adhesion

Volumn 14, Issue 4, 2007, Pages 157-167

  Takata, Masahiko ^a   Maniwa, Yoshimasa ^a   Doi, Takefumi ^a   Tanaka, Yugo ^a   Okada, Kenji ^a   Nishio, Wataru ^a   Ohbayashi, Chiho ^a   Yoshimura, Masahiro ^b   Hayashi, Yoshitake ^a   Okita, Yutaka ^a  

^a KOBE UNIVERSITY HOSPITAL   (Japan)

^b HYOGO CANCER CENTER   (Japan)

Author keywords

Assays; Cancer stroma interaction; Cell invasion; Collagen gel; Double layer collagen gel; Double layered collagen gel hemisphere; E cadherin; Proteolysis; Three dimensional

Indexed keywords

COLLAGEN GEL; COLLAGEN TYPE 1; PROTEINASE;

ARTICLE; CANCER CELL; CELL ACTIVITY; CELL ASSAY; CELL INFILTRATION; CELL INTERACTION; CELL INVASION; CELL KINETICS; CONTROLLED STUDY; CULTURE MEDIUM; EXTRACELLULAR MATRIX; FIBROBLAST; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IN VITRO STUDY; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; STROMA CELL; THREE DIMENSIONAL IMAGING;

ADENOCARCINOMA; ANTIGENS, CD44; BIOLOGICAL ASSAY; CADHERINS; CELL ADHESION; CELL MOVEMENT; COLLAGEN; EXTRACELLULAR MATRIX; GELS; HUMANS; LUNG NEOPLASMS; MATRIX METALLOPROTEINASES; NEOPLASM INVASIVENESS; RNA, SMALL INTERFERING; STROMAL CELLS; TUMOR CELLS, CULTURED;

EID: 35548982155     PISSN: 15419061     EISSN: 15435180     Source Type: Journal    
DOI: 10.1080/15419060701557859     Document Type: Article

References (23)

1. Albini, A., Iwamoto, Y., Kleinman, H. K., Martin, G. R., Aaronson, S. A., Kozlowski, J. M. and McEwan, R. N. (1987) A rapid in vitro assay for quantitating the invasive potential of tumor cells. Cancer Res, 47, pp. 3239-3245.
2. Bajorath, J. (2001) Structure and function of CD44: Characteristic molecular features and analysis of the hyaluronan binding site. Results Probl Cell Differ, 33, pp. 85-103.
3. Beningo, K. A., Dembo, M. and Wang, Y. (2004) Responses of fibroblasts to anchorage of dorsal extracellular matrix receptors. Proc Natl Acad Sci, 101, pp. 18024-18029.
4. Bremnes, R. M., Veve, R., Gabrielson, E., Hirsch, F. R., Baron, A., Bemis, L., Gemmill, R. M., Drabkin, H. A. and Franklin, W. A. (2002) High-throughput tissue microarray analysis used to evaluate biology and prognostic significance of the E-cadherin pathway in non-small-cell lung cancer. J Clin Oncol, 20, pp. 2417-2428.
5. Cavallaro, U. and Christofori, G. (2004) Cell adhesion and signalling by cadherins and Ig-CAMs in cancer. Nat Rev Cancer, 4, pp. 118-132.
6. Conacci-Sorrell, M., Zhurinsky, J. and Ben-Ze'ev, A. (2002) The cadherin-catenin adhesion system in signaling and cancer. J Clin Invest, 109, pp. 987-991.
7. Cukierman, E., Pankov, R., Stevens, D. R. and Yamada, K. M. (2001) Taking cell-matrix adhesions to the third dimension. Science, 294, pp. 1708-1712.
8. Demou, Z. N. and McIntire, L. V. (2002) Fully Automated Three-Dimensional Tracking of Cancer Cells in Collagen Gels: Determination of Motility Phenotypes at the Cellular Level. Cancer Res, 62, pp. 5301-5307.
9. Duong, H. S., Le, A. D., Zhang, Q. and Messadi, D. V. (2005) A novel 3-dimensional culture system as an in vitro model for studying oral cancer cell invasion. Int J Exp Pathol, 86, pp. 365-374.
10. Even-Ram, S. and Yamada, K. M. (2005) Cell migration in 3-D matrix. Curr Opin Cell Biol, 17, pp. 524-532.
11. Kanomata, N., Nakahara, R., Oda, T., Aoyagi, Y., Ishii, G., Yokose, T., Hasebe, T., Nagai, K., Yokozaki, H. and Ochiai, A. (2005) Expression and localization of mRNAs for matrix metalloproteinases and their inhibitors in mixed bronchioloalveolar carcinomas with invasive components. Mod Pathol, 18, pp. 828-837.
12. Kimura, K., Endo, Y., Yonemura, Y., Heizmann, C. W., Schafer, B. W., Watanabe, Y. and Sasaki, T. (2000) Clinical significance of S100A4 and E-cadherin-related adhesion molecules in non-small cell lung cancer. Int J Oncol, 16, pp. 1125-1131.
13. Lim, S. C., Jang, I. G., Kim, Y. C. and Park, K. O. (2000) The role of E-cadherin expression in non-small cell lung cancer. J Korean Med Sci, 15, pp. 501-506.
14. Liotta, L. A. and Kohn, E. C. (2001) The microenvironment of the tumour-host interface. Nature, 411, pp. 375-379.
15. Liu, D., Huang, C., Kameyama, K., Hayashi, E., Yamauchi, A., Kobayashi, S. and Yokomise, H. (2001) E-cadherin expression associated with differentiation and prognosis in patients with non-small cell lung cancer. Ann Thorac Surg, 71, pp. 949-954.
16. Noguchi, M., Morikawa, A., Kawasak, M., Matsuno, Y., Yamada, T., Hirohashi, S., Kondo, H. and Shimosato, Y. (1995) Small adenocarcinoma of the lung. Histologic characteristics and prognosis. Cancer, 75, pp. 2844-2852.
17. Nystrom, M. L., Thomas, G. J., Stone, M., Mackenzie, I. C., Hart, J. F. and Marshall, J. F. (2005) Development of a quantitative method to analyse tumour cell invasion in organotypic culture. J Pathol, 205, pp. 468-475.
18. Shibanuma, H., Hirano, T., Tsuji, K., Shrestha, B., Konaka, C., Ebihara, Y. and Kato, H. (1998) Influence of E-cadherin dysfunction upon local invasion and metastasis in non-small cell lung cancer. Lung Cancer, 22, pp. 85-95.
19. Suetsugu, S., Yamazaki, D., Kurisu, S. and Takenawa, T. (2003) Differential roles of WAVE1 and WAVE2 in dorsal and peripheral ruffle formation for fibroblast cell migration. Dev Cell, 5, pp. 595-609.
20. Tokunou, M., Niki, T., Eguchi, K., Iba, S., Tsuda, H., Yamada, T., Matsuno, Y., Kondo, H., Saitoh, Y., Imamura, H. and Hirohashi, S. (2001) c-MET expression in myofibroblasts: Role in autocrine activation and prognostic significance in lung adenocarcinoma. Am J Pathol, 158, pp. 1451-1463.
21. Weber, G. F., Bronson, R. T., Ilagan, J., Cantor, H., Schmits, R. and Mak, T. W. (2002) Absence of the CD44 gene prevents sarcoma metastasis. Cancer, 62, pp. 2281-2286.
22. Werbowetski, T., Bjerkvig, R. and Maestro, R F Del (2004) Evidence for a secreted chemorepellent that directs glioma cell invasion. J Neurobiol, 60, pp. 71-88.
23. Wolf, K., Mazo, I., Leung, H., Engelke, K., von Andrian, U. H., Deryugina, E. I., Strongin, A. Y., Brocker, E. B. and Friedl, P. (2003) Compensation mechanism in tumor cell migration: Mesenchymal-amoeboid transition after blocking of pericellular proteolysis. J Cell Biol, 160, pp. 267-277.