Bioactive molecules from amphibian skin: Their biological activities with reference to therapeutic potentials for possible drug development

Indian Journal of Experimental Biology

Volumn 45, Issue 7, 2007, Pages 579-593

  Gomes, Antony ^a   Giri, Biplab ^a   Saha, Archita ^a   Mishra, R ^a   Dasgupta, Subir C ^a   Debnath, A ^b   Gomes, Aparna ^b  

^a UNIVERSITY OF CALCUTTA   (India)

^b INDIAN INSTITUTE OF CHEMICAL BIOLOGY   (India)

Author keywords

Amphibians; Frog skin; Medicinal application; Skin bioactive molecules; Therapeutic potential; Toad skin

Indexed keywords

AMPHIBIA; ANURA;

ANTIDIABETIC AGENT; ANTIINFECTIVE AGENT; ANTINEOPLASTIC AGENT; ANTIVIRUS AGENT; BIOMATERIAL; CARDIOTONIC AGENT; PEPTIDE;

AMPHIBIA; ANIMAL; CHEMISTRY; DRUG DESIGN; FUNCTIONS OF THE SKIN AND ITS APPENDAGES; HUMAN; METABOLISM; REVIEW; SKIN;

AMPHIBIA; ANIMALS; ANTI-INFECTIVE AGENTS; ANTINEOPLASTIC AGENTS; ANTIVIRAL AGENTS; BIOCOMPATIBLE MATERIALS; CARDIOTONIC AGENTS; DRUG DESIGN; HUMANS; HYPOGLYCEMIC AGENTS; PEPTIDES; SKIN; SKIN PHYSIOLOGY;

EID: 35548977688     PISSN: 00195189     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (136)

1. Duellman W E & Trueb L, in Biology of amphibians (McGraw-Hill, New York) 1986, 257.
2. Bhuiyan M B, Fant M E & Dasgupta A, Study on mechanism of action of Chinese medicine Chan Su: Dose-dependent biphasic production of nitric oxide in trophoblastic BeWo cells, Clin Chim Acta, 330 (2003) 179.
3. Ko W S, Park T Y, Park C, Kim Y H, Yoon H J, Lee S Y, Hong S H, Choi B T, Lee Y T & Choi YH, Induction of apoptosis by Chan Su, a traditional Chinese medicine, in human bladder carcinoma T24 cells, Oncol Rep, 14 (2005) 475.
4. Shimizu Y, Inoue E & Ito C, Effect of the water-soluble and non-dialyzable fraction isolated from Senso (Chan Su) on lymphocyte proliferation and natural killer activity in C3H mice, Biol Pharm Bull, 27 (2004) 256.
5. Clarke B T, The natural history of amphibian skin secretions, their normal functioning and potential medicinal applications, Biol Rev, 72 (1997) 365.
6. Morishita S, Shoji M, Oguni Y, Ito C, Noguchi K & Sakanashi M, Congestive heart failure model in rabbits: effects of digoxin and a drug containing toad venom, Jpn. J Pharmacol, 56 (1991) 427.
7. Morishita S, Shoji M, Oguni Y, Ito C, Noguchi K & Sakanashi M, Effects of "kyushin", a drug containing toad venom, on experimental congestive heart failure in rabbits. Am J Chin Med, 20 (1992) 83.
8. Ojiri Y, Chibana T, Noguchi K & Sakanashi M, Cardiovascular effect of a senso (toad venom) - containing drug in anesthetized dogs (2): Influence of propranolol, Am J Chin Med, 20 (1992) 147.
9. Morishita S, Sugimoto C, Shoji M, Hirai Y, Oguni Y, Ito C, Higuchi M & Sakanashi M, Pharmacological actions of "kyushin, " a drug containing toad venom (3): Effects on experimentally induced arrhythmia, Am J Chin Med, 21 (1993) 139.
10. Sakanashi M, Noguchi K, Chibana T, Ojiri Y & Shoji M, Effects of intraduodenal administration of "kyushin, " a senso (toad venom)-containing drug, on systemic hemodynamics, cardiacfunction and myocardial oxygen consumption in anesthetized dogs, Am J Chin Med, 21 (1993) 7.
11. Yamahara J, Tanaka S, Matsuda H, Sawada T & Fujimura H, The mode of cardiac action of cardiotonic steroids isolated from Toad Cake in perfused working guinea-pig heart and effect of cinobufagin on experimental heart failure, Nippon Yakurigaku Zasshi, 88 (1986) 413.
12. Pamnani M B, Chen S, Bryant H J, Schooley J F Jr, Eliades D C, Yuan C M & Haddy F J, Effects of three sodiumpotassium adenosine triphosphatase inhibitors, Hypertension, 18 (1991) 316.
13. Hirai Y, Morishita S, Ito C, & Sakanashi M, Effects of bufadienolides and some kinds of cardiotonics on guinea pig hearts, Nippon Yakurigaku Zasshi, 100 (1992) 127.
14. Yuan C, Manunta P, Chen S, Hamlyn J M, Haddy F J & Pamnani M B, Role of ouabain-like factors in hypertension: effects of ouabain and certain endogenous ouabain-like factors in hypertension, J Cardiovasc Pharmacol, 22 (1993) S10.
15. Bagrov A Y, Roukoyatkina N I, Pinaev A G, Dmitrieva R I & Fedrova O V, Effects of two endogenous Na+, K(+)- ATPase inhibitors, marinobufagenin and ouabain, on isolated rat aorta, Eur J Pharmacol, 274 (1995) 151.
16. Matsukawa M, Akizawa T, Morris J F, Butler V P Jr & Yoshioka M, Marinoic acid, a novel bufadienolide-related substance in the skin of the giant toad, Bufo marinus, Chem Pharm Bull (Tokyo), 44 (1996) 255.
17. Matsukawa M, Akizawa T, Ohigashi M, Morris J F, Butler V P Jr & Yoshioka M, A novel bufadienolide, marinosin, in the skin of the giant toad, Bufo marinus, Chem Pharm Bull (Tokyo), 45 (1997) 249.
18. Orlov B N & Krylov V N, Effect of the venom of the toad Bufo viridis Laur. on cardiac hemodynamics in dogs, Nauchnye Doki Vyss Shkoly Biol Nauki, 4 (1986) 54.
19. Marenah L, Flatt P R, Orr D F, McClean S, Shaw C & Abdel-Wahab Y H, Skin secretion of the toad Bombina variegata contains multiple insulin-releasing peptides including bombesin and entirely novel insulinotropic structures, Biol Chem, 385 (2004) 315.
20. Marenah L, Flatt P R, Orr D F, McClean S, Shaw C & Abdel-Wahab Y H, Brevinin-1 and multiple insulin-releasing peptides in the skin of the frog Rana palustris, J Endocrinol, 181 (2004) 347.
21. Marenah L, McClean S, Flatt P R, Orr D F, Shaw C & Abdel-Wahab Y H, Novel insulin-releasing peptides in the skin of Phyllomedusa trinitatis frog include 28 amino acid peptide from dermaseptin BIV precursor, Pancreas, 29 (2004) 110.
22. Marenah L, Shaw C, Orr D F, McClean S, Flatt P R & Abdel-Wahab Y H, Isolation and characterisation of an unexpected class of insulinotropic peptides in the skin of the frog Agalychnis litodryas, Regu. Pept, 120 (2004) 33.
23. Abdel-Wahab Y H, Marenah L, Orr D F, Shaw C & Flatt P R Isolation and structural characterisation of a novel 13-amino acid insulin-releasing peptide from the skin secretion of Agalychnis calcarifer, Biol Chem, 386 (2005) 581.
24. Marenah, L.; Flatt, P.R.; Orr, D.F.; Shaw, C.; Abdel-Wahab, Y.H. Characterization of naturally occurring peptides in the skin secretion of Rana pipiens frog reveal pipinin-1 as the novel insulin-releasing agent, J Pept Res, 66 (2005) 204.
25. Marenah L, Flatt P R, Orr D F, Shaw C & Abdel-Wahab Y H, Isolation and structural characterization of novel Rugosin A-like insulinotropic peptide from the skin secretions of Rana saharica frog, Peptides 26 (2005) 2117.
26. Marenah L, Flatt P R, Orr D F, Shaw C & Abdel-Wahab Y H, Skin secretions of Rana saharica frogs reveal antimicrobial peptides esculentins-1 and -1B and brevinins- 1E and -2EC with novel insulin releasing activity, J Endocrinol, 188 (2006) 1.
27. Yue B B, High dose cinobufocini in attenuation and treatment of infection and granulocytopenia during combinedchemotherapy of malignant blood diseases, Chung-Kuo- Chung-Hsi-I-Chieh-Ho-Tsa-Chih, 12 (1992) 145.
28. Das M, Dasgupta S C & Gomes A, Toad (Bufo melanostictus, Schneider) skin extract induced haematological and biochemical changes in rodents, Indian J Pharmacol, 30 (1998) 68.
29. Das M, Dasgupta S C & Gomes A, Immunomodulatory and antineoplastic activity of common Indian toad (Bufo melanostictus, Schneider) skin extract, Indian J Pharmacol, 30 (1998) 311.
30. Salmon A L, Cross L J, Irvine A E, Lappin T R, Dathe M, Krause G, Canning P, Thim L, Beyermann M, Rothemund S, Bienert M & Shaw C, Peptide leucine arginine, a potent immunomodulatory peptide isolated and structurally characterized from the skin of the Northern Leopard frog, Rana pipiens, J Biol Chem, 276 (2001) 10145.
31. Graham C, Irvine A E, McClean S, Richter S C, Flatt P R & Shaw C, Peptide Tyrosine Arginine, a potent immunomodulatory peptide isolated and structurally characterized from the skin secretions of the dusky gopher frog, Rana sevosa, Peptides, 26 (2005) 737.
32. Barberio C, Delfino G & Mastromei G, A low molecular weight protein with antimicrobial activity in the cutaneous 'venom' of the yellow-bellied toad (Bombina variegata pachypus), Toxicon, 25 (1987) 899.
33. Zasloff M, Magainins, a class of antimicrobial peptides from Xenopus skin: isolation, characterization of two active forms, and partial cDNA sequence of precursor, Proc Nat Acad Sci USA, 84 (1987) 5449.
34. Soravia E, Martini G & Zasloff M, Antimicrobial properties of peptides from Xenopus granular gland secretions, FEBS Lett, 228 (1988) 337.
35. Cuervo J H, Rodriguez B & Houghten R A, The Magainins: sequence factors relevant to increased antimicrobial activity and decreased hemolytic activity, Pept Res, 1 (1988) 81.
36. Westerhoff H V, Juretic D, Hendler R W & Zasloff M, Magainins and the disruption of membrane-linked freeenergy transduction, Proc Natl Acad Sci USA, 86 (1989) 6597.
37. Mor A, Nguyen V H, Delfour A, Migliore-Samour D & Nicolas P, Isolation, amino acid sequence, and synthesis of dermatoxin from frog skin, an antibacterial peptide encoded by a novel member of the dermaseptin genes family, Eur J Biochem, 267 (2000) 4583.
38. Mor A, Amiche M & Nicolas P, Structure, synthesis, and activity of dermaseptin b, a novel vertebrate defensive peptide from frog skin: Relationship with adenoregulin, Biochemistry, 33 (1994), 6642.
39. Amiche M, Seon A A, Wroblewski H & Nicolas P, Isolation of dermatoxin from frog skin, an antibacterial peptide encoded by a novel member of the dermaseptin genes family, Eur J Biochem, 267 (2000), 4583.
40. Brand G D, Leite J R, Silva L P, Albuquerque S, Prates M V, Azevedo R B, Carregaro V, Silva J S, Sa V C, Brandao R A & Bloch C Jr, Dermaseptins from Phyllomedusa oreades and Phyllomedusa distincta. Anti-Trypanosoma cruzi activity without cytotoxicity to mammalian cells, J Biol Chem, 277 (2002) 49332.
41. Amiche M, Ducancel F, Lajeunesse E, Boulain J C, Menez A & Nicolas P, Molecular cloning of a cDNA encoding the precursor of adenoregulin from frog skin. Relationships with the vertebrate defensive peptides, dermaseptins, Biochem Biophys Res Commun, 191 (1993) 983.
42. Leite J R, Silva L P, Rodrigues M I, Prates M V, Brand G D, Lacava B M, Azevedo R B, Bocca A L, Albuquerque S & Bloch C Jr, Phylloseptins: a novel class of anti-bacterial and anti-protozoan peptides from the Phyllomedusa genus.Peptides, 26 (2005) 565.
43. Simmaco M, Barra, D, Chiarini F, Noviello L, Melchiorri P, Kreil G & Richter K, A family of bombinin-related peptides from the skin of Bombina variegata, Eur J Biochem, 199 (1991) 217.
44. Gibson BW, Tang D Z, Mandrell R, Kelly M & Spindel E R, Bombinin-like peptides with antimicrobial activity from skin secretions of the Asian toad, Bombina orientalis, J Biol Chem, 266 (1991) 23103.
45. Lai R, Zheng Y T, Shen J H, Liu G J, Liu H, Lee W H, Tang S Z & Zhang Y, Antimicrobial peptides from skin secretions of Chinese red belly toad Bombina maxima, Peptides, 23 (2002) 427.
46. Li J, Xu X, Yu H, Yang H, Huang Z & Lai R, Direct antimicrobial activities of PR-bombesin, Life Sci, 78 (2006) 1953.
47. Morikawa N, Hagiwara K & Nakajima T, Brevinin-1 and -2, unique antimicrobial peptides from the skin of the frog, Rana brevipoda porsa, Biochem Biophys Res Commun, 189 (1992) 184.
48. Simmaco M, Mignogna G, Barra D & Bossa F, Novel antimicrobial peptides from skin secretion of the European frog Rana esculenta, FEBS Lett, 324 (1993) 159.
49. Conlon J M, Al-Ghaferi N, Abraham B, Sonnevend A, Coquet L, Leprince J, Jouenne T, Vaudry H & Iwamuro S, Antimicrobial peptides from the skin of the Tsushima brown frog Rana tsushimensis, Comp Biochem Physiol C Toxicol Pharmacol, 143 (2006) 42.
50. Clark D P, Durell S, Maloy W L & Zasloff, M, Ranalexin - A novel antimicrobial peptide from bullfrog (Rana catesbeiana) skin, structurally related to the bacterial antibiotic, polymyxin, J Biol Chem, 269 (1994) 10849.
51. Park J M, Jung J E & Lee B J, Antimicrobial peptides from the skin of a Korean frog, Rana rugosa. Biochem Biophys Res Commun, 205(1994), 948. [Erratum in: Biochem Biophys Res Commun, 209 (1995) 775].
52. Suzuki S, Ohe Y, Okubo T, Kakegawa T & Tatemoto K, Isolation and characterization of novel antimicrobial peptides, rugosins A, B and C, from the skin of the frog, Rana rugosa, Biochem Biophys Res Commun, 212 (1995) 249.
53. Simmaco M, Mignogna G, Canofeni S, Miele R, Mangoni M L & Barra D, Temporins, antimicrobial peptides from the European red frog Rana temporaria. Eur J Biochem, 242 (1996) 788.
54. Goraya J, Knoop F C & Conlon J M, Ranatuerins: Antimicrobial peptides isolated from the skin of the American bullfrog, Rana catesbeiana, Biochem Biophys Res Commun, 250 (1998) 589.
55. Goraya J, Knoop F C & Conlon J M, Ranatuerin 1T: An antimicrobial peptide isolated from the skin of the frog, Rana temporaria, Peptides, 20 (1999) 159.
56. Halverson T, Basir Y J, Knoop F C & Conlon J M, Purification and characterization of antimicrobial peptidesfrom the skin of the North American green frog Rana clamitans, Peptides, 21 (2000) 469.
57. Sai K P, Jagannadham M V, Vairamani M, Raju N P, Devi A S, Nagaraj R & Sitaram N, Tigerinins: a novel antimicrobial peptide isolated from Indian frog rana tigerina, J Biol Chem, 276 (2001) 2701.
58. Park S, Park, S H, Ahn H C, Kim S, Kim S S, Lee B J & Lee B J, Structural study of novel antimicrobial peptides, nigrocins, isolated from Rana nigromaculata, FEBS Lett, 507 (2001) 95.
59. Isaacson T, Soto A, Iwamuro S, Knoop F C & Conlon J M, Antimicrobial peptides with atypical structural features from the skin of the Japanese brown frog Rana japonica, Peptides, 23 (2002) 419.
60. Kim J B, Iwamuro S, Knoop F C & Conlon J M, Antimicrobial peptides from the skin of the Japanese mountain brown frog, Rana ornativentris, J Pept Res, 58(2001), 349.
61. Ali M F, Lips K R, Knoop F C, Fritzsch B, Miller C & Conlon J M, Antimicrobial peptides and protease inhibitors in the skin secretions of the crawfish frog, Rana areolata, Biochim Biophys Acta, 1601 (2002) 55.
62. Mangoni M L, Papo N, Mignogna G, Andreu D, Shai Y, Barra D & Simmaco M, Ranacyclins, a new family of short cyclic antimicrobial peptides: biological function, mode of action, and parameters involved in target specificity, Biochemistry, 42 (2003) 14023.
63. Conlon J M, Sonnevend A, Patel M, Al-Dhaheri K, Nielsen P F, Kolodziejek J, Nowotny N, Iwamuro S & Pal T, A family of brevinin-2 peptides with potent activity against Pseudomonas aeruginosa from the skin of the Hokkaido frog, Rana pirica, Regul Pept, 118 (2004) 135.
64. Xu X, Li J, Han Y, Yang H, Liang J, Lu Q & Lai R, Two antimicrobial peptides from skin secretions of Rana grahami, Toxicon, 47 (2006) 459.
65. Conlon J M, Al-Ghaferi N, Abraham B, Jiansheng H, Cosette P, Leprince J, Jouenne T & Vaudry H, Antimicrobial peptides from diverse families isolated from the skin of the Asian frog, Rana grahami, Peptides, 27 (2006) 2111.
66. Raftery M J, Waugh R J, Bowie J H, Wallace J C & Tyler M J, The structures of the frenatin peptides from the skin secretion of the giant tree frog Litoria infrafrenata, J Pept Sci, 2 (1996) 117.
67. Steinborner S T, Currie, G J, Bowie J H, Wallace J C & Tyler M J, New antibiotic caerin 1 peptides from the skin secretion of the Australian tree frog Litoria chloris. Comparison of the activities of the caerin 1 peptides from the genus Litoria, J Pept Res, 51 (1998) 121.
68. Apponyi M A, Pukala T L, Brinkworth C S, Maselli V M, Bowie J H, Tyler M J, Booker G W, Wallace J C, Carver J A, Separovic F, Doyle J & Llewellyn L E, Host-defence peptides of Australian anurans: structure, mechanism of action and evolutionary significance, Peptides, 25 (2004) 1035.
69. Rozek T, Waugh R J, Steinborner S T, Bowie J H, Tyler M J & Wallace J C, The maculatin peptides from the skin glands of the tree frog Litoria genimaculata: a comparison of the structures and antibacterial activities of maculatin 1.1 and caerin 1.1, J Pept Sci, 4 (1998) 111.
70. Wabnitz P A, Bowie J H, Wallace J C & Tyler M J, The citropin peptides from the skin glands of the Australian Blue Mountains tree frog Litoria citropa. Part 2: sequence determination using electrospray mass spectrometry, Rapid Commun Mass Spectrom, 13 (1999) 1724.
71. Mattute B, Knoop F C &Conlon J M, Kassinatuerin-1: A peptide with broad-spectrum antimicrobial activity isolated from the skin of the hyperoliid frog, Kassina senegalensis, Biochem Biophys Res Commun, 268 (2000) 433.
72. Olson L 3rd, Soto A M, Knoop F C & Conlon J M, Pseudin- 2: An antimicrobial peptide with low hemolytic activity from the skin of the paradoxical frog, Biochem Biophys Res Commun, 288 (2001) 1001.
73. Rollins-Smith L A, King J D, Nielsen P F, Sonnevend A & Conlon J M, An antimicrobial peptide from the skin secretions of the mountain chicken frog Leptodactylus fallax (Anura: Leptodactylidae), Regul Pept, 124 (2005) 173.
74. King J D, Al-Ghaferi N, Abraham B, Sonnevend A, Leprince J, Nielsen P F & Conlon J M, Pentadactylin: an antimicrobial peptide from the skin secretions of the South American bullfrog Leptodactylus pentadactylus, Comp Biochem Physiol C Toxicol Pharmacol, 141 (2005) 393.
75. Dourado F S, Moreira K G, Brand G D, Melo J T, Leite J R, Silva L P, Bloch C Jr & Schwartz, E.F, In Syphaxin 1.5, a novel antimicrobial peptide isolated from the skin secretions of the anuran Leptodactylus syphax. I5th World Congress on Animal, Plant and Microbial toxins, held at Glasgow, Scotland, July 23-28, 2006, edited by Harvey, A. (Elsevier: Amsterdam) 2006.
76. Cunha Filho G A, Schwartz C A, Resck I, Murta M M, Lemos S S, Castro M S, Kyaw C, Pires O R Jr, Leite J R, Bloch C Jr & Schwartz E F, Antimicrobial activity of the bufadienolides marinobufagin and telocinobufagin isolated as major components from skin secretion of the toad Bufo rubescens, Toxicon, 45 (2005) 777.
77. Hernandez C, Mor A, Dagger F, Nicolas P, Hernandez A, Benedetti E L & Dunia I, Functional and structural damage in Leishmania mexicana exposed to the cationic peptide dermaseptin, Eur J Cell Biol, 59 (1992) 414.
78. Mangoni M L, Saugar J M, Dellisanti M, Barra D, Simmaco M & Rivas L, Temporins, small antimicrobial peptides with leishmanicidal activity, J Biol Chem, 280 (2005) 984.
79. Krugliak M, Feder R, Zolotarev V Y, Gaidukov L, Dagan A, Ginsburg H & Mor A, Antimalarial activities of dermaseptin S4 derivatives, Antimicrob Agents Chemother, 44 (2000) 2442.
80. 80Yasin B, Pang M, Turner J S, Cho Y, Dinh N N, Waring A J, Lehrer R I & Wagar E A, Evaluation of the inactivation of infectious Herpes simplex virus by host-defense peptides, Eur J Clin Microbiol Infect Dis, 19 (2000) 187.
81. Chinchar V G, Wang J, Murti G, Carey C & Rollins-Smith L, Inactivation of frog virus 3 and channel catfish virus by esculentin-2P and ranatuerin-2P, two antimicrobial peptides isolated from frog skin, Virology, 288 (2001) 351.
82. Belaid A, Aouni M, Khelifa R, Trabelsi A, Jemmali M & Hani K, In vitro antiviral activity of dermaseptins against herpes simplex virus type 1, J Med Virol, 66 (2002) 229.
83. Zhao Y, Jin Y, Wang J H, Wang R R, Yang L M, Lee W H, Zheng Y T & Zhang Y, A novel heme-containing protein with anti-HIV-1 activity from skin secretions of Bufo andrewsi, Toxicon, 46 (2005) 619.
84. Bradbury J, Frog skin hope for HIV prevention, Drug Discov Today, 10 (2005) 1489.
85. Cruciani R A, Barker J L, Zasloff M, Chen H & Colamonici O, Antibiotic magainins exert cytolytic activity against transformed cell lines through channel formation, Pro Nat Acad Sci USA, 88 (1991) 3792.
86. Ohsaki Y, Gazdar A F, Chen H C & Johnson B E Antitumor activity of magainin analogues against human lung cancer cell lines, Cancer Res, 52 (1992) 3534.
87. Balboni F, Bernabei P A, Barberio C, Sanna A, Rossi Ferrini P & Delfino G, Cutaneous venom of Bombina variegata pachypus (Amphibia, Anura): effects on the growth of the human HL 60 cell line, Cell Biol Int Rep, 16 (1992) 329.
88. Doyle J, Brinkworth C S, Wegener K L, Carver J A, Llewellyn L E, Olver I N, Bowie J H, Wabnitz P A & Tyler M J, nNOS inhibition, antimicrobial and anticancer activity of the amphibian skin peptide, citropin1.1 and synthetic modifications, Eur J Biochem, 270 (2003) 1141.
89. Kamano Y, Nogawa T, Yamashita A, Hayashi, M, Inoue M, Drasar P & Pettit G R, Isolation and structure of a 20, 21- epoxybufenolide series from "Ch'an Su", J Nat Prod, 65 (2002) 1001.
90. Zhang L S, Nayaka K, Yoshida T & Kuroiwa Y, Bufalin as a potent inducer of differentiation of human myeloid leukaemia cells, Biochem Biophys Res Comm, 178 (1991) 686.
91. Zhang L Nakaya K, Yoshida T & Kuroiwa Y, Induction by bufalin of differentiation of human leukemia cells HL60, U937, and ML1 toward macrophage/monocyte-like cells and its potent synergistic effect on the differentiation of human leukemia cells in combination with other inducers. Cancer Res, 53 (1993) 934.
92. Jing Y, Watabe M, Hashimoto S, Nakajo S & Nakaya K, Cell cycle arrest and protein kinase modulating effect of bufalin on human leukemia ML1 cells, Anticancer Res, 14(1994), 1193.
93. Hashimoto S, Jing Y, Kawazoe N, Masuda Y, Nakajo S, Yoshida T, Kuroiwa Y & Nakaya K, Bufalin reduces the level of topoisomerase II in human leukemia cells and affects the cytotoxicity of anticancer drugs, Leuk Res, 21 (1997) 875.
94. Numazawa S, Shinoki M A, Ito H, Yoshida T & Kuroiwa Y, Involvement of Na+, K(+)-ATPase inhibition in K562 cell differentiation induced by bufalin, J Cell Physiol, 160(1994), 113.
95. Akiyama M, Ogura M, Iwai M, Iijima M, Numazawa S & Yoshida T, Effect of bufalin on growth and differentiation of human skin carcinoma cells in vitro, Hum Cell, 12 (1999) 205.
96. Zhang L, Yoshida T & Kuroiwa Y, Stimulation of melanin synthesis of B16-F10 mouse melanoma cells by bufalin, Life Sci, 51 (1992) 17.
97. Yeh J Y, Huang W J, Kan S F & Wang P S, Effects of bufalin and cinobufagin on the proliferation of androgen dependent and independent prostate cancer cells, Prostrate, 54 (2003) 112.
98. Nasu K, Nishida M, Ueda T, Takai N, Bing S, Narahara, H & Miyakawa I, Bufalin induces apoptosis and the G0/G1 cell cycle arrest of endometriotic stromal cells: a promising agent for the treatment of endometriosis, Mol Hum Reprod, 11 (2005) 817.
99. Giri B & Gomes A, Antineoplastic activity of Indian toad (Bufo melanostictus, Schneider) skin extract on EAC cells and Human leukemic (U937 & HL60) cell line, Indian J Pharmacol, 36 (2004) S83.
100. Giri B, Gomes A, Debnath A, Saha A, Biswas A K, Dasgupta, S C & Gomes A, Antiproliferative, cytotoxic and apoptogenic activity of Indian toad (Bufo melanostictus, Schneider) skin extract on U937 and K562 cells, Toxicon, 48 (2006), 388.
101. Gomes A, Giri B, Kole L, Saha A, Debnath A & Gomes A, A crystalline compound (BM-ANF1) from the Indian toad (Bufo melanostictus, Schneider) skin extract, induced antiproliferation & apoptosis in leukemic & hepatoma cell line involving cell cycle proteins, Toxicon (2007) (Unpublished).
102. Montecucchi P C, Gozzini L, Erspamer V & Melchiorri P, The primary structure of tryptophan containing peptides from skin extracts of Phyllomedusa rhodei (tryptophyllins), Int J Peptide Res, 24 (1984) 276.
103. Renda T, D'Este L, Buffa R, Usellini L, Capella C, Vaccaro R & Melchiorri P, Tryptophyllin-like immunoreactivity in rat adenohypophysis, Peptides, 6 Suppl 3 (1985) 197.
104. Das M, Auddy B & Gomes A, Pharmacological study of the toad skin extract on experimental animals. Indian J Pharmacol, 28 (1996) 72.
105. Das M, Malllick B N, Dasgupta S C & Gomes A, A sleep inducing factor from common Indian toad (Bufo melanostictus, Schneider) skin extract, Toxicon, 38 (2000) 1267.
106. Montecucchi P C, de Castiglione R, Piani S, Gozzini L & Erspamer V, Amino acid composition and sequence of dermorphin, a novel opiate-like peptide from the skin of Phyllomedusa sauvagei, Int J Pept Protein Res, 17 (1981) 275.
107. Montecucchi P C, de Castiglione R & Erspamer V, Identification of dermorphin and Hyp6-dermorphin in skin extracts of the Brazilian frog Phyllomedusa rhodei, Int J Pept Protein Res, 17 (1981) 316.
108. Broccardo M, Erspamer V, Falconieri Erspamer G, Improta G, Linari G, Melchiorri P & Montecucchi P C, Pharmacological data on dermorphins, a new class of potent opioid peptides from amphibian skin, Br J Pharmacol, 73 (1981) 625.
109. Puglisi-Allegra S, Castellano, C, Filibeck, U, Oliverio A & Melchiorri P, Behavioural data on dermorphins in mice, Eur J Pharmacol, 82 (1982) 223.
110. Braga P C, Tiengo M, Biella G, Dall'Oglio G & Fraschini F, Dermorphin, a new peptide from amphibian skin, inhibits the nociceptive thalamic neurons firing rate evoked by noxious stimuli, Neurosci Lett, 52 (1984)165.
111. Zanoni C I S, Molska G R, Zaharenko A J, Paraventi C, Jimenez R S, Paula-Freire L I G, Parada C A, Fereira S H, Malpezzi-Marinho E L A & Marinho E A V, In - ê and ì opiod receptors are involved in the antinociceptive activity of methanolic skin extract of Phyllomedusa rhodei on the hot plate model. I5th World Congress on Animal, Plant and Microbial toxins, held at Glasgow, Scotland, July 23-28, 2006, edited by Harvey, A. (Elsevier: Amsterdam), 2006.
112. Spande T F, Garraffo H M, Edwards M W, Yeh H J C, Panel L & Daly J W, Epibatidine: A Novel (Chloropyridyl)azabicycloheptane with potent analgesic activity from an ecuadoran poison frog, J Am Che. Soc, 114 (1992) 3475.
113. Qian C, Li T, Shen T Y, Libertine-Garahan L, Eckman J, Biftu T & Ip S, Epibatidine is a nicotinic analgesic, Eur J Pharmacol, 250 (1993) R13.
114. Badio B & Daly J W, Epibatidine, a Potent Analgesic and Nicotinic Agonist, Mol Pharmacol, 45 (1994) 563.
115. Fisher M, Huangfu D, Shen T Y & Guyenet P G, Epibatidine, an alkaloid from the poison frog Epipedobates tricolor, is a powerful ganglionic depolarizing agent, J Pharmacol Exp Ther, 270 (1994) 702.
116. Wang G, Sun G, Tang W & Pan X, The application of traditional Chinese medicine to the management of hepatic cancerous pain, J Tradit Chin Med, 14 (1994) 132.
117. Doyle J, Llewellyn L E, Brinkworth C S, Bowiem J H, Wegener K L, Rozek T, Wabnitz P A, Wallace J C & Tyler M J, Amphibian peptides that inhibit neuronal nitric oxide synthase. isolation of lesuerin from the skin secretion of the Australian Stony Creek frog Litoria lesueuri, Eur J Biochem, 269 (2002) 100.
118. Edelstein M C, Gretz J E, Bauer T J, Fulgham D L, Alexander N J & Archer D F, Studies on the in vitro spermicidal activity of synthetic magainins, Fertil Steril, 55 (1991) 647.
119. Reddy K V, Shahani S K, Meherji P K, Spermicidal activity of Magainins: In vitro and in vivo studies, Contraception, 53 (1996) 205.
120. Clara A, Manjramkar D D & Reddy V K, Preclinical evaluation of magainin-A as a contraceptive antimicrobial agent, Fertil Steril, 81 (2004) 1357.
121. Mystokowa E T Niemierko A Komar A & Sawicki W, Embryotoxicity of magainin-2-amide and its enhancement by cyclodextrin, albumin, hydrogen peroxide and acidification, Hum. Reprod, 16 (2001) 1457.
122. Zairi A Belaid A, Gahbiche A & Hani K, Spermicidal activity of dermaseptins, Contraception, 72 (2005) 447.
123. Jackson I M & Reichlin S, Thyrotropin-releasing hormone: Abundance in the skin of the frog, Rana pipiens, Science, 198 (1977) 414.
124. Montecucchi P C & Henschen A, Amino acid composition and sequence analysis of sauvagine, a new active peptide from the skin of Phyllomedusa sauvagei, Int J Pept Protein Res, 18 (1981) 113.
125. Rossi A, di Salle E, Briatico G, Arcari G, de Castiglione R & Perseo G, Antinociceptive, prolactin releasing and intestinal motility inhibiting activities of dermorphin and analogues after subcutaneous administration in the rat, Peptides, 4 (1983) 577.
126. Cocchi D, Degli Uberti E C, Trasforini G, Salvadori S, Tomatis R, Torpia R & Perelli-Cippo R, Prolactin releasing and luteinizing hormone inhibiting activity of dermorphin shorter homologues in the rat, Life Sci, 36 (1985) 1707.
127. Gullner H G & Kelly G D, Dermorphin: Effects on anterior pituitary function in the rat, Arch Int Pharmacodyn Ther, 262 (1983) 208.
128. Gullner H G, Owen W W, Harris V & Unger R H, Dermorphin: An opioid peptide from amphibian skin which affects endocrine pancreatic function, Arch Int Pharmacodyn Ther, 266 (1983) 155.
129. Montecucchi P C, Isolation and primary structure determination of amphibian skin tryptophyllins, Peptides, 6 (1985) 187.
130. Negri L, Lattanzi R, Giannini E, De Felice M, Colucci A & Melchiorri P, Bv8, the amphibian homologue of the mammalian prokineticins, modulates ingestive behaviour in rats, Br J Pharmacol, 142 (2004) 181.
131. Silva R W, Zaharenko A J, Paula-Freire L I G, Almeida E, Frussa-Filho R, Malpezzi-Marinho E L A & Marinho E A V, In - Activity of extracts from Phyllomedusa rhodei (Amphibia, Anura) skin on the mice behaviour in the open field and rota rod. I5th World Congress on Animal, Plant and Microbial toxins, held at Glasgow, Scotland, July 23-28, 2006, edited by Harvey, A. (Elsevier: Amsterdam) 2006.
132. Sai K P, Reddy P N & Babu M, Investigations on wound healing by using amphibian skin, Indian J Exp Biol, 33 (1995) 673.
133. Kumar K V, Sai K P & Babu M, Application of frog (Rana tigerina Daudin) skin collagen as novel substrate in cell culture, J Biomed Mater Res, 61 (2002) 197.
134. Zhang J, Zhang Y, Wan S G, Wei S S, Lee W H & Zhang Y, Bm-TFF2, a trefoil factor protein with platelet activation activity from frog Bombina maxima skin secretions, Biochem Biophys Res Commun, 330 (2005) 1027.
135. Fredericks L P & Dankert J R, Antibacterial and hemolytic activity of the skin of the terrestrial salamander, Plethodon cinereus, J Exp Zool, 287 (2000) 340.
136. Lai R, Yang D M, Lee W H & Zhang Y, Biological activities of skin secretions of the salamander Tylototriton verrucosus, J Nat Toxins, 11 (2002) 245.