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This review discusses chromatin organisation from the point of view of high-resolution data from electron spectroscopic imaging. Chromosomes are seen mostly composed by 10 nm and 30 nm fibres, forming a network of fibres that spread throughout the nucleus. On the basis of these observations, the authors propose the 'lattice model' of chromosome organisation.
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This paper shows for the first time non-random, directional movement of a locus within the nucleus. Using an inducible transgenic system that allows tracking of activated loci by live-cell microscopy, the authors show fast migration of loci from the nuclear periphery to the interior upon activation (1-5 μm in 1-2 h). This movement is shown to be dependent on actin and myosin I.
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Chuang C.H., Carpenter A.E., Fuchsova B., Johnson T., de Lanerolle P., and Belmont A.S. Long-range directional movement of an interphase chromosome site. Curr Biol 16 (2006) 825-831. This paper shows for the first time non-random, directional movement of a locus within the nucleus. Using an inducible transgenic system that allows tracking of activated loci by live-cell microscopy, the authors show fast migration of loci from the nuclear periphery to the interior upon activation (1-5 μm in 1-2 h). This movement is shown to be dependent on actin and myosin I.
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Using a tandem gene array in mouse cells, this study demonstrates the architecture of an active subchromosomal domain. RNA polymerases and nascent transcripts cluster at the core of the domain, whereas intervening sequences form a chromatin cloud around the active core.
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In vivo identification of genomic regions that interact with B-type lamin in Drosophila using DamID technology. The genes identified are mainly transcriptionally silent, late replicating and do not contain active epigenetic marks. Binding of genes to lamin is shown to be dependent on transcriptional activity, histone modifications and is developmentally regulated.
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This is one of three papers that describes a genome-wide approach for the identification of long-range interactions of a given locus with the remaining genome. The murine β-globin locus control region (LCR) is shown to interact mainly with active regions of the genome in fetal liver (where the LCR is active) but with inactive regions in brain (where the LCR is inactive). Conversely, a housekeeping gene, Rad23a, interacts with active regions of the genome in both tissues.
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Simonis M., Klous P., Splinter E., Moshkin Y., Willemsen R., de Wit E., van Steensel B., and de Laat W. Nuclear organization of active and inactive chromatin domains uncovered by chromosome conformation capture-on-chip (4C). Nat Genet 38 (2006) 1348-1354. This is one of three papers that describes a genome-wide approach for the identification of long-range interactions of a given locus with the remaining genome. The murine β-globin locus control region (LCR) is shown to interact mainly with active regions of the genome in fetal liver (where the LCR is active) but with inactive regions in brain (where the LCR is inactive). Conversely, a housekeeping gene, Rad23a, interacts with active regions of the genome in both tissues.
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This paper suggests that long-range chromatin interactions involving a single enhancer H element (on chromosome 14) and a single olfactory receptor (OR) gene (amongst ∼1300 genes spread throughout the genome) correlate with the choice of the specific olfactory receptor gene that is expressed in a given OR neuron. Transgenic insertion of additional H elements results in the expression of more than one olfactory receptor gene in each cell. The interaction occurs in 85% of transcribing alleles, though this represents only 30% of all expressing cells, suggesting an intermittent behaviour of transcription.
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