The inherent property of polyhydroxyalkanoate synthase to form spherical PHA granules at the cell poles: The core region is required for polar localization

Journal of Biotechnology

Volumn 132, Issue 3, 2007, Pages 238-245

  Peters, Verena ^a   Becher, Dorit ^a   Rehm, Bernd H A ^a  

^a MASSEY UNIVERSITY   (New Zealand)

Author keywords

Cupriavidus necator; Mutagenesis; PHA; PHA synthase; PHB; Polar localization; Polyhydroxyalkanoate; Polyhydroxybutyrate

Indexed keywords

CELLS; ENZYME ACTIVITY; MOLECULAR STRUCTURE; MUTAGENESIS;

CUPRIAVIDUS NECATOR; PHA SYNTHASE; POLAR LOCALIZATION; POLYHYDROXYALKANOATE; POLYHYDROXYBUTYRATE;

ESTERS;

AMPICILLIN; ANTIBIOTIC AGENT; CHLORAMPHENICOL; DNA POLYMERASE; GENOMIC DNA; KANAMYCIN; POLY(3 HYDROXYBUTYRIC ACID); POLYESTER; POLYHYDROXYALKANOATE SYNTHASE; POLYHYDROXYALKANOIC ACID; TETRACYCLINE;

ARTICLE; BACTERIAL GROWTH; CELL GRANULE; CELL POLARITY; CELLULAR DISTRIBUTION; ENZYME ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME LOCALIZATION; ESCHERICHIA COLI; FLUORESCENCE MICROSCOPY; HYDROPHOBICITY; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL;

ACYLTRANSFERASES; CELL POLARITY; CUPRIAVIDUS NECATOR; ESCHERICHIA COLI; GREEN FLUORESCENT PROTEINS; HEMAGGLUTININS; MICROSCOPY, FLUORESCENCE; MUTAGENESIS, INSERTIONAL; MUTANT PROTEINS; MUTATION; POLYHYDROXYALKANOATES; PROTEIN TRANSPORT; RECOMBINANT FUSION PROTEINS;

CUPRIAVIDUS NECATOR;

EID: 35448964302     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2007.03.001     Document Type: Article

References (37)

1. Amara A.A., Steinbüchel A., and Rehm B.H.A. In vivo evolution of the Aeromonas punctata polyhydroxyalkanoate (PHA) synthase: isolation and characterization of modified PHA synthases with enhanced activity. Appl. Microbiol. Biotechnol. 59 (2002) 477-482
2. Amara A.A., and Rehm B.H.A. Replacement of the catalytic nucleophile cysteine-296 by serine in class II polyhydroxyalkanoate synthase from Pseudomonas aeruginosa-mediated synthesis of a new polyester: identification of catalytic residues. Biochem. J. 374 (2003) 413-421
3. Baeckstroem T.B., Brockelbank J.A., and Rehm B.H.A. Recombinant Escherichia coli produces tailor-made biopolyester granules for applications in fluorescence activated cell sorting: functional display of the mouse interleukin-2 and myelin oligodendrocyte glycoprotein. BMC Biotechnol. 7 (2007) 3
4. Brandl H., Gross R.A., Lenz R.W., and Fuller R.C. Pseudomonas oleovorans as a source of poly(beta-hydroxyalkanoates) for potential applications as biodegradable polyesters. Appl. Environ. Microbiol. 54 (1988) 1977-1982
5. Brockelbank J.A., Peters V., and Rehm B.H.A. Recombinant Escherichia coli strain produces a ZZ domain displaying biopolyester granules suitable for immunoglobulin G purification. Appl. Environ. Microbiol. 72 (2006) 7394-7397
6. Bullock W.O., Fernandez J.M., and Short J.M. Xl1-Blue-a high-efficiency plasmid transforming reca Escherichia coli strain with beta-galactosidase selection. Biotechniques 5 (1987) 376
7. Dunlop W.F., and Robards A.W. Ultrastructural study of poly-hydroxybutyrate granules from bacillus cereus. J. Bacteriol. 114 (1973) 1271-1280
8. Gerngross T.U., Snell K.D., Peoples O.P., Sinskey A.J., Csuhai E., Masamune S., and Stubbe J. Overexpression and purification of the soluble polyhydroxyalkanoate synthase from Alcaligenes eutrophus: evidence for a required posttranslational modification for catalytic activity. Biochemistry 33 (1994) 9311-9320
9. Hoffmann N., Amara A.A., Br. Beermann B., Hinz H.-J., and Rehm B.H.A. Biochemical characterization of the Pseudomonas putida transacylase which diverts intermediates of fatty acid de novo biosynthesis. J. Biol. Chem 277 (2002) 42926-42936
10. Hoppensack A., Rehm B.H.A., and Steinbüchel A. Analysis of 4-phosphopantetheinylation of polyhydroxybutyrate synthase from Ralstonia eutropha: generation of beta-alanine auxotrophic Tn5 mutants and cloning of the panD gene region. J. Bacteriol. 181 (1999) 1429-1435
11. Jendrossek D. Fluorescence microscopical investigation of poly(3-hydroxybutyrate) granule formation in bacteria. Biomacromolecules 6 (2005) 598-603
12. Jensen T.E., and Sicko L.M. Fine structure of poly-beta-hydroxybutyric acid granules in a blue-green alga, Chlorogloea fritschii. J. Bacteriol. 106 (1971) 683-686
13. Jia Y., Kappock T.J., Frick T., Sinskey A.J., and Stubbe J. Lipases provide a new mechanistic model for polyhydroxybutyrate (PHB) synthases: characterization of the functional residues in Chromatium vinosum PHB synthase. Biochemistry 39 (2000) 3927-3936
14. Kalousek S., Dennis D., and Lubitz W. Genetic engineering of PHB synthase from Alcaligenes eutrophus H16. FEMS Microbiol. Rev. 103 (1992) 426-427
15. Kovach M.E., Phillips R.W., Elzer P.H., Roop R.M., and Peterson K.M. pBBR1MCS-a broad-host-range cloning vector. Biotechniques 16 (1994) 800-802
16. Lundgren D.G., Pfister R.M., and Merrick J.M. Structure of poly-beta-hydroxybutyric acid granules. J. Gen. Microbiol. 34 (1964) 441-446
17. Madison L.L., and Huisman G.W. Metabolic engineering of poly(3-hydroxyalkanoates): from DNA to plastic. Microbiol. Mol. Biol. Rev. 63 (1999) 21-53
18. Mayer F., Madkour M.H., PieperFurst U., Wieczorek R., Liebergesell M.L., and Steinbüchel A. Electron microscopic observations on the macromolecular organization of the boundary layer of bacterial PHA inclusion bodies. J. Gen. Appl. Microbiol. 42 (1996) 445-455
19. Miller W.G., Leveau J.H., and Lindow S.E. Improved gfp and inaZ broad-host-range promoter-probe vectors. Mol. Plant Microbe Interact. 13 (2000) 1243-1250
20. Normi Y.M., Hiraishi T., Taguchi S., Abe H., Sudesh K., Najimudin N., and Doi Y. Characterization and properties of G4X mutants of Ralstonia eutropha PHA synthase for poly(3-hydroxybutyrate) biosynthesis in Escherichia coli. Macromol. Biosci. 5 (2005) 197-206
21. Peters V., and Rehm B.H.A. In vivo monitoring of PHA granule formation using GFP-labeled PHA synthases. FEMS Microbiol. Lett. 248 (2005) 93-100
22. Peters V., and Rehm B.H.A. In vivo enzyme immobilization by use of engineered polyhydroxyalkanoate synthase. Appl. Environ. Microbiol. 72 (2006) 1777-1783
23. Rehm B.H.A., and Hancock R.E.W. Membrane topology of the outer membrane protein OprH from Pseudomonas aeruginosa: PCR-mediated site-directed insertion and deletion mutagenesis. J. Bacteriol. 178 (1996) 3346-3349
24. Rehm B.H.A., Qi Q., Br. Beermann B., Hinz H.-J., and Steinbüchel A. Matrix-assisted in vitro refolding of Pseudomonas aeruginosa class II polyhydroxyalkanoate synthase from inclusion bodies produced in recombinant Escherichia coli. Biochem. J. 358 (2001) 263-268
25. Rehm B.H.A., Antonio R.V., Spiekermann P., Amara A.A., and Steinbuchel A. Molecular characterization of the poly(3-hydroxybutyrate) (PHB) synthase from Ralstonia eutropha: in vitro evolution, site-specific mutagenesis and development of a PHB synthase protein model. Biochim. Biophys. Acta 1594 (2002) 178-190
26. Rehm B.H.A. Polyester synthases: natural catalysts for plastics. Biochem. J. 376 (2003) 15-33
27. Rehm B.H.A. Genetics and biochemistry of polyhydroxyalkanoate granule self-assembly: the key role of polyester synthases. Biotechnol. Lett. 28 (2006) 207-213
28. Rehm, B.H.A. (Ed.), 2006b. Biopolyester particles produced by microbes and their potential applications. In: Microbial Bionanotechnology: Biological Self-Assembly Systems and Biopolymer-Based Nanostructures, pp. 1-34.
29. Rehm B.H.A. Biogenesis of microbial polyhydroxyalkanoate granules: a platform technology for the production of tailor-made bioparticles. Curr. Issues Mol. Biol. 9 (2007) 41-62
30. Sambrook J., Fritsch E.F., and Maniatis T. Molecular Cloning: A Laboratory Manual (1989), Cold Spring Harbor Lab. Press, Plainview, NY
31. Schubert P., Krüger N., and Steinbüchel A. Molecular analysis of the Alcaligenes eutrophus poly(3-hydroxybutyrate) biosynthetic operon: identification of the N terminus of poly(3-hydroxybutyrate) synthase and identification of the promoter. J. Bacteriol. 173 (1991) 168-175
32. Spiekermann P., Rehm B.H.A., Kalscheuer R., Baumeister D., and Steinbüchel A. A sensitive, viable-colony staining method using Nile Red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds. Arch. Microbiol. 171 (1999) 73-80
33. Taguchi S., Nakamura H., Hiraishi T., Yamato I., and Doi Y. In vitro evolution of a polyhydroxybutyrate synthase by intragenic suppression-type mutagenesis. J. Biochem. (Tokyo) 131 (2002) 801-806
34. Tian J., Sinskey A.J., and Stubbe J. Kinetic studies of polyhydroxybutyrate granule formation in Wautersia eutropha H16 by transmission electron microscopy. J. Bacteriol. 187 (2005) 3814-3824
35. Tsuge T., Saito Y., Narike M., Muneta K., Normi Y.M., Kikkawa Y., Hiraishi T., and Doi Y. Mutation effects of a conserved alanine (Ala510) in type I polyhydroxyalkanoate synthase from Ralstonia eutropha on polyester biosynthesis. Macromol. Biosci. 4 (2004) 963-970
36. Yuan W., Jia Y., Tian J., Snell K.D., Muh U., Sinskey A.J., Lambalot R.H., Walsh C.T., and Stubbe J. Class I and III polyhydroxyalkanoate synthases from Ralstonia eutropha and Allochromatium vinosum: characterization and substrate specificity studies. Arch. Biochem. Biophys. 394 (2001) 87-98
37. Zheng Z., Li M., Xue X.J., Tian H.L., Li Z., and Chen G.Q. Mutation on N-terminus of polyhydroxybutyrate synthase of Ralstonia eutropha enhanced PHB accumulation. Appl. Microbiol. Biotechnol. 72 (2006) 896-905