Chimerogenesis in estimation of specificity and pathway directions for cytochrome P45017α catalyzed reactions

Biochemistry (Moscow)

Volumn 69, Issue 4, 2004, Pages 364-375

  Gilep, A A ^a   Estabrook, R W ^b   Usanov, S A ^a  

^a INSTITUTE OF BIOORGANIC CHEMISTRY   (Belarus)

^b UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

Chimeric proteins; CYP17; Cytochrome P450; Cytochrome P45017 ; Steroidogenesis

Indexed keywords

AMINO ACIDS; BIOSYNTHESIS; CATALYST ACTIVITY; HORMONES; POLYPEPTIDES; PROTEINS; RATE CONSTANTS;

CHIMERIC PROTEINS; CYTOCHROME P450; CYTOCHROME P45017Α; STEROIDGENESIS;

BIOCHEMISTRY;

ESCHERICHIA COLI;

HYBRID PROTEIN; STEROID 17ALPHA MONOOXYGENASE;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CATTLE; CHEMISTRY; ENZYME STABILITY; ESCHERICHIA COLI; GENE DELETION; GENE EXPRESSION; GENE VECTOR; GENETICS; HORSE; KINETICS; METABOLISM; MOLECULAR GENETICS; MOUSE; NUCLEOTIDE SEQUENCE; PLASMID; RAT; SEQUENCE ALIGNMENT; SIGNAL TRANSDUCTION; SWINE;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CATTLE; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; HORSES; KINETICS; MICE; MOLECULAR SEQUENCE DATA; PLASMIDS; RATS; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE DELETION; SIGNAL TRANSDUCTION; STEROID 17-ALPHA-HYDROXYLASE; SWINE;

EID: 3543085569     PISSN: 00062979     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:BIRY.0000026190.80583.c3     Document Type: Article

References (32)

1. Hanukoglu, I. (1992) J. Steroid Biochem. Mol. Biol., 43, 779-804.
2. Bhasker, C. R., Adler, B. S., Dee, A., John, M. E., Kagimoto, M., Zuber, M. X., Ahlgren, R., Wang, X. D., Simpson, E. R., and Waterman, M. R. (1989) Arch. Biochem. Biophys., 271, 479-487.
3. Lorence, M. C., Trant, J. M., Clark, B. J., Khyatt, B., Mason, J. I., Estabrook, R. W., and Waterman, M. R. (1990) Biochemistry, 29, 9819-9824.
4. Nakajin, S., Takahashi, M., Shinoda, M., and Hall, P. F. (1985) Biochem. Biophys. Res. Commun., 132, 708-713.
5. Beaudoin, C., Lavallee, B., Tremblay, Y., Hum, D. W., Breton, R., de Launoit, Y., and Belanger, A. (1998) DNA Cell Biol., 17, 707-715.
6. Shinzawa, K., Kominami, S., and Takemori, S. (1985) Biochim. Biophys. Acta, 833, 151-160.
7. Swart, P., Estabrook, R. W., Mason, J. I., and Waterman, M. R. (1989) Biochem. Soc. Trans., 17, 1025-1026.
8. Lee-Robiohaud, P., Wright, J. N., Akhtar, M. E., and Akhtar, M. (1995) Biochem. J., 308 (Pt. 3), 901-908.
9. Barnes, H. J., Arlotto, M. P., and Waterman, M. R. (1991) Proc. Natl. Acad. Sci. USA, 88, 5597-5601.
10. Buczko, E., Koh, Y. C., Miyagawa, Y., and Dufau, M. L. (1995) J. Steroid Biochem. Mol. Biol., 52, 209-218.
11. Kitamura, M., Buczko, E., and Dufau, M. L. (1991) Mol. Endocrinol., 5, 1373-1380.
12. Nakajin, S., Hall, P. F., and Onoda, M. (1981) J. Biol. Chem., 256, 6134-6139.
13. Ishii-Ohba, H., Matsumura, R., Inano, H., and Tamaoki, B. (1984) J. Biochem. (Tokyo), 95, 335-343.
14. Chudaev, M. V., Chemogolov, A. A., and Usanov, S. A. (1997) 17th Int. Conf. Biochem. Mol. Biol., pp. 24-29.
15. Shen, A. L., Porter, T. D., Wilson, T. E., and Kasper, C. B. (1989) J. Biol. Chem., 264, 7584-7589.
16. Laemmli, U. K. (1970) Nature, 227, 680-685.
17. Towbin, H., Staehelin, T., and Gordon, J. (1979) Pros. Natl. Acad. Sci. USA, 76, 4350-4354.
18. Omura, T., and Sato, R. (1964) J. Biol. Chem., 239, 2370-2378.
19. Katagiri, M., Kagawa, N., and Waterman, M. R. (1995) Arch. Biochem. Biophys., 317, 343-347.
20. Fardella, C. E., Hum, D. W., Homoki, J., and Miller, W. L. (1994) J. Clin. Endocrinol. Metab., 79, 160-164.
21. Lam, C. W., Arlt, W., Chan, C. K., Honour, J. W., Lin, C. J., Tong, S. F., Choy, K. W., and Miller, W. L. (2001) Mol. Genet. Metab., 72, 254-259.
22. Monno, S., Ogawa, H., Date, T., Fujioka, M., Miller, W. L., and Kobayashi, M. (1993) J. Biol. Chem., 268, 25811-25817.
23. Geller, D. H., Auchus, R. J., and Miller, W. L. (1999) Mol. Endocrinol., 13, 167-175.
24. Sakaki, T., Shibata, M., Yabusaki, Y., and Ohkawa, H. (1987) DNA, 6, 31-39.
25. Pompon, D. (1988) Eur. J. Biochem., 177, 285-293.
26. Brock, B. J., and Waterman, M. R. (2000) Arch. Biochem. Biophys., 373, 401-408.
27. Lindberg, R. L., and Negishi, M. (1989) Nature, 339, 632-634.
28. Auchus, R. J., and Miller, W. L. (1999) Mol. Endocrinol., 13, 1169-1182.
29. Gotoh, O. (1992) J. Biol. Chem., 267, 83-90.
30. Loughran, P. A., Roman, L. J., Aitken, A. E., Miller, R. T., and Masters, B. S. (2000) Biochemistry, 39, 15110-15120.
31. Podust, L. M., Stojan, J., Poulos, T. L., and Waterman, M. R. (2001) J. Inorg. Biochem., 87, 227-235.
32. Roussel, F., Khan, K. K., and Halpert, J. R. (2000) Arch. Biochem. Biophys., 374, 269-278.