Human 17β-hydroxysteroid dehydrogenases types 1, 2, and 3 catalyze bi-directional equilibrium reactions, rather than unidirectional metabolism, in HEK-293 cells

Archives of Biochemistry and Biophysics

Volumn 429, Issue 1, 2004, Pages 50-59

  Khan, Naveed ^a   Sharma, Kamalesh K ^a   Andersson, Stefan ^a   Auchus, Richard J ^a  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

Androgens; Estrogens; Hydroxysteroid dehydrogenase; Pyridine nucleotide cofactor; Steroid metabolism; Transactivation

Indexed keywords

17 OXOSTEROID; 17BETA HYDROXYSTEROID DEHYDROGENASE TYPE 1; 17BETA HYDROXYSTEROID DEHYDROGENASE TYPE 2; 17BETA HYDROXYSTEROID DEHYDROGENASE TYPE 3; ANDROGEN; ESTRADIOL; ESTROGEN; ESTRONE; ISOTOPE; OXIDOREDUCTASE; TESTOSTERONE 17BETA DEHYDROGENASE; UNCLASSIFIED DRUG; 3 (OR 17) BETA HYDROXYSTEROID DEHYDROGENASE; 3 (OR 17)-BETA-HYDROXYSTEROID DEHYDROGENASE; HYDROXYSTEROID DEHYDROGENASE; RECOMBINANT PROTEIN; STEROID;

ARTICLE; CATALYSIS; CELLULAR DISTRIBUTION; CHEMICAL REACTION; CONTROLLED STUDY; CULTURE MEDIUM; EMBRYO; EMBRYO CELL; ENZYME ASSAY; ENZYME METABOLISM; FIBROBLAST CULTURE; GENE MUTATION; HUMAN; HUMAN CELL; INCUBATION TIME; KIDNEY CELL CULTURE; KINETICS; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; THERMODYNAMICS; TRANSACTIVATION; WILD TYPE; CELL LINE; COMPARATIVE STUDY; ENZYME ACTIVATION; ENZYME SPECIFICITY; ENZYMOLOGY; GENETIC TRANSFECTION; GENETICS; KIDNEY; METABOLIC CLEARANCE RATE; METABOLISM; PRENATAL DEVELOPMENT;

17-HYDROXYSTEROID DEHYDROGENASES; CATALYSIS; CELL LINE; ENZYME ACTIVATION; HUMANS; KIDNEY; KINETICS; METABOLIC CLEARANCE RATE; RECOMBINANT PROTEINS; STEROIDS; SUBSTRATE SPECIFICITY; TRANSFECTION;

EID: 3543068881     PISSN: 00039861     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.abb.2004.05.023     Document Type: Article

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