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Four different antibodies (S21, S23, S36, and S53) were tested in epitopemapping experiments. We found that S53 as the primary antibody and S36 as the second antibody were the pair that allowed for the most sensitive and specific S100ββ detection (data not shown).
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Four different antibodies (S21, S23, S36, and S53) were tested in epitopemapping experiments. We found that S53 as the primary antibody and S36 as the second antibody were the pair that allowed for the most sensitive and specific S100ββ detection (data not shown).
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The noise level (N) is calculated as the standard deviation on a blank injection and was found to be 0.3 Hz. The intercept (I) and the sensitivity (S) are determined from the first linear part of the dose-response curve. I is given as the value where the linear curve crosses the Y axis, whereas S is reported as the slope of the linear curve.
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The noise level (N) is calculated as the standard deviation on a blank injection and was found to be 0.3 Hz. The intercept (I) and the sensitivity (S) are determined from the first linear part of the dose-response curve. I is given as the value where the linear curve crosses the Y axis, whereas S is reported as the slope of the linear curve.
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More information on the streptavidin MPs used in this article can be found on the company websites (http://www.ademtech.com and images of all of particles used in this study are shown in the Supporting Information Figure S2
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More information on the streptavidin MPs used in this article can be found on the company websites (http://www.ademtech.com and http://www.in- vitrogen.com). SEM images of all of particles used in this study are shown in the Supporting Information (Figure S2).
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For the detection of magnetic particles via microscope imaging, the detection limit (DL) can be calculated using the same formula as given in eq 1. In the case of microscope detection, the noise (N) is defined as the coverage of particles at 0 ng/mL S100ββ, as reported in Table 2. I is given as the value where the linear curve crosses the y axis and S is given as the slope of the linear part of the dose-response curve. Both N and I are given in percent and S is given in percent mL/ng.
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For the detection of magnetic particles via microscope imaging, the detection limit (DL) can be calculated using the same formula as given in eq 1. In the case of microscope detection, the noise (N) is defined as the coverage of particles at 0 ng/mL S100ββ, as reported in Table 2. I is given as the value where the linear curve crosses the y axis and S is given as the slope of the linear part of the dose-response curve. Both N and I are given in percent and S is given in percent mL/ng.
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