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Volumn 79, Issue 19, 2007, Pages 7540-7548

The optimization of magnetosandwich assays for the sensitive and specific detection of proteins in serum

Author keywords

[No Author keywords available]

Indexed keywords

ASSAYS; BIOSENSORS; MAGNETIC FIELD EFFECTS; OPTICAL MICROSCOPY; QUARTZ CRYSTAL MICROBALANCES; SURFACE CHEMISTRY;

EID: 35349006828     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac0713407     Document Type: Article
Times cited : (26)

References (55)
  • 8
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    • Wirix-Speetjens, R.; De Boeck, J. In Encyclopedia of Sensors; Grimes, C. A., Dickey, E. C., Pishko, M. V.; American Scientific Publishers: Valencia, CA, 2006; 5, pp 403-414.
    • Wirix-Speetjens, R.; De Boeck, J. In Encyclopedia of Sensors; Grimes, C. A., Dickey, E. C., Pishko, M. V.; American Scientific Publishers: Valencia, CA, 2006; vol. 5, pp 403-414.
  • 34
    • 35348967185 scopus 로고    scopus 로고
    • The MPs applied in this article are given a name based on their supplier, and the hydrodynamic diameter is measured in our lab by dynamic light scattering as reported in a previous publication (ref 48).
    • The MPs applied in this article are given a name based on their supplier, and the hydrodynamic diameter is measured in our lab by dynamic light scattering as reported in a previous publication (ref 48).
  • 35
    • 0031317867 scopus 로고    scopus 로고
    • Prior to use, the MPs were washed three times with HBS and were finally dissolved at a concentration of 1 mg/mL. (36) Rodahl, M.; Höök, F.; Fredriksson, C.; Keller, C. A.; Mozer, A.; Brzezinski, P.; Voinova, M.; Kasemo, B. Faraday Discuss. 1997, 107, 229.
    • Prior to use, the MPs were washed three times with HBS and were finally dissolved at a concentration of 1 mg/mL. (36) Rodahl, M.; Höök, F.; Fredriksson, C.; Keller, C. A.; Mozer, A.; Brzezinski, P.; Voinova, M.; Kasemo, B. Faraday Discuss. 1997, 107, 229.
  • 44
    • 35348965526 scopus 로고    scopus 로고
    • Four different antibodies (S21, S23, S36, and S53) were tested in epitopemapping experiments. We found that S53 as the primary antibody and S36 as the second antibody were the pair that allowed for the most sensitive and specific S100ββ detection (data not shown).
    • Four different antibodies (S21, S23, S36, and S53) were tested in epitopemapping experiments. We found that S53 as the primary antibody and S36 as the second antibody were the pair that allowed for the most sensitive and specific S100ββ detection (data not shown).
  • 45
    • 35349016170 scopus 로고    scopus 로고
    • The noise level (N) is calculated as the standard deviation on a blank injection and was found to be 0.3 Hz. The intercept (I) and the sensitivity (S) are determined from the first linear part of the dose-response curve. I is given as the value where the linear curve crosses the Y axis, whereas S is reported as the slope of the linear curve.
    • The noise level (N) is calculated as the standard deviation on a blank injection and was found to be 0.3 Hz. The intercept (I) and the sensitivity (S) are determined from the first linear part of the dose-response curve. I is given as the value where the linear curve crosses the Y axis, whereas S is reported as the slope of the linear curve.
  • 48
    • 35348987962 scopus 로고    scopus 로고
    • More information on the streptavidin MPs used in this article can be found on the company websites (http://www.ademtech.com and images of all of particles used in this study are shown in the Supporting Information Figure S2
    • More information on the streptavidin MPs used in this article can be found on the company websites (http://www.ademtech.com and http://www.in- vitrogen.com). SEM images of all of particles used in this study are shown in the Supporting Information (Figure S2).
  • 51
    • 35348976463 scopus 로고    scopus 로고
    • Fluidic Force Discrimination
    • U.S. Patent 0,253,-744, December 16, 2004
    • Rife, J. C.; Whitman, L. J. Fluidic Force Discrimination. U.S. Patent 0,253,-744, December 16, 2004.
    • Rife, J.C.1    Whitman, L.J.2
  • 53
    • 35348963967 scopus 로고    scopus 로고
    • For the detection of magnetic particles via microscope imaging, the detection limit (DL) can be calculated using the same formula as given in eq 1. In the case of microscope detection, the noise (N) is defined as the coverage of particles at 0 ng/mL S100ββ, as reported in Table 2. I is given as the value where the linear curve crosses the y axis and S is given as the slope of the linear part of the dose-response curve. Both N and I are given in percent and S is given in percent mL/ng.
    • For the detection of magnetic particles via microscope imaging, the detection limit (DL) can be calculated using the same formula as given in eq 1. In the case of microscope detection, the noise (N) is defined as the coverage of particles at 0 ng/mL S100ββ, as reported in Table 2. I is given as the value where the linear curve crosses the y axis and S is given as the slope of the linear part of the dose-response curve. Both N and I are given in percent and S is given in percent mL/ng.
  • 54
    • 0003625084 scopus 로고
    • Nakamura, R, Kasahara, Y, Rechnitz, G. A, American Society for Microbiology: Washington D.C
    • Immunochemical Assays and Biosensor Technologies for the 1990s; Nakamura, R.; Kasahara, Y.; Rechnitz, G. A.; American Society for Microbiology: Washington D.C., 1992.
    • (1992) Immunochemical Assays and Biosensor Technologies for the 1990s


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.