Preparation of a light-sensitive and reversible dissolution copolymer and its application in lysozyme purification

Biotechnology Progress

Volumn 23, Issue 5, 2007, Pages 1124-1129

  Wen, Wen ^a   Wan, Junfen ^a   Cao, Xuejun ^a   Xia, Jinan ^b  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b DONGHUA UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

COPOLYMERIZATION; DISSOLUTION; ENZYME ACTIVITY; LASER BEAM EFFECTS; SYNTHESIS (CHEMICAL);

CRYLIC ACID; LIGHT DENSITY;

COPOLYMERS;

BIOMATERIAL; LYSOZYME; POLYMER;

ARTICLE; CHEMISTRY; ISOLATION AND PURIFICATION; LIGHT; METHODOLOGY; PHOTOCHEMISTRY; RADIATION DOSE; RADIATION EXPOSURE;

BIOCOMPATIBLE MATERIALS; LIGHT; MURAMIDASE; PHOTOCHEMISTRY; POLYMERS; RADIATION DOSAGE;

EID: 35348908900     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp070114f     Document Type: Article

References (15)

1. Gil, E. S.; Hudson, S. M. Stimuli-responsive polymer and their bioconjugates. Prog. Polym. Sci. 2004, 29, 1173-1222.
2. Hassan, A.; Muallem, A.; Mohamed, I. M. W.; Asrof, A. S. K. Synthesis and solytion properties of a new ionic polymer and its behavior in aqueous two-phase polymer systems. Polymer 2002, 43, 1041-1050.
3. Cao, X. J.; Qin, W. pH-sensitive and reusable zwitterionic polytmer forming aqueous two-phase systems. CHN Patent 1693325A, 2005.
4. Persson, J.; Kaul, A.; Tjerneld, F. Polymer recycling in aqueous two-phase extractions using thermoseparating ethylene oxide-propylene oxide copolymers. J. Chromatogr. B 2000, 743, 115-126.
5. Mamada, T.; Tanaka, D.; Irie, K. M. Photoinduced phase transition of gels. Macromolecules 1991, 23, 1517-1519.
6. Suzuki, A.; Tanaka, T. Phase transition in polymer gels of induced by visible light. Nature 1990, 346, 345-347.
7. Xiaohua, L.; Xiaogong, W.; deshan, L. Synthesis and characterization of photo-responsive and thermosensitive polymer. Acta Polym. Sin. 2001, 6, 773-777.
8. Kong, F. Q.; Cao, X. J.; Xia, J. A. Synthesis and application of a light-sensitive polymer forming aqueous two-phase systems. J. Ind. Eng. Chem. 2007, 13 (3), 424-428.
9. Owen, R. O. Direct purification of lysozyme using continuous counter-current expanded bed adsorption. J. Chromatogr. 1997, 757, 41-45.
10. Vaidya, A. A. Thermo precipitation of lysozyme from egg white using copolymers of N-isopropylacrylamide and acidic monomers. J. Biotechnol. 2001, 87, 95-107.
11. Dehai, L.; Yujie, C. Facility detection of lysozyme activity. Chin. Dairy Ind. 2002, 30, 128-129.
12. Qingping, J.; Yeqing, L.; Ruifang, Y. Biochemical Engineering Experiment; Science and Technology University of East China: Shanghai, 2000; p 128.
13. Yuping, Z.; yanjun, Y. The improvement of method measuring lysozyme. Food Sci. (Chin.) 2002, 23, 116-119.
14. Melander, W.; Horvath, C. Salt effects on hydrophobic interactions in precipitation and chromatography of proteins: an interpretation of hydrophobic series. Arch. Biochem. Biophys. 1977, 183, 200-215.
15. Ehsani, N.; Parkkinen, S.; Nystrom, M. Fractionation of natural and model egg-white protein solutions with modified and unmodified polysulfone UF membranes. J. Membr. Sci. 1997, 123, 105-119.