Using respirometric techniques and fluorescent in situ hybridization to evaluate the heterotrophic active biomass in activated sludge

Biotechnology and Bioengineering

Volumn 98, Issue 3, 2007, Pages 561-568

  Ismail, A ^a   Wentzel, M C ^b   Bux, F ^a  

^a DURBAN UNIVERSITY OF TECHNOLOGY   (South Africa)

^b UNIVERSITY OF CAPE TOWN   (South Africa)

Author keywords

Ammonia oxidizing bacteria; Fluorescent in situ hybridization; Heterotrophic active biomass; Modified batch test; Nitrite oxidizing bacteria

Indexed keywords

ACTIVATED SLUDGE PROCESS; AMMONIA; CALIBRATION; KINETIC PARAMETERS; MATHEMATICAL MODELS; OLIGONUCLEOTIDES; WASTEWATER TREATMENT;

AMMONIA OXIDIZING BACTERIA; FLUORESCENT IN SITU HYBRIDIZATION; HETEROTROPHIC ACTIVE BIOMASS; MODIFIED BATCH TEST; NITRITE OXIDIZING BACTERIA;

BIOMASS;

4',6 DIAMIDINO 2 PHENYLINDOLE; AMMONIA; DNA 16S;

ARTICLE; BACTERIUM; BACTERIUM CULTURE; BIOMASS; CONTROLLED STUDY; DISTRIBUTION VOLUME; FLUORESCENCE IN SITU HYBRIDIZATION; HETEROTROPHY; KINETICS; MATHEMATICAL MODEL; MICROBIAL POPULATION DYNAMICS; MOLECULAR PROBE; NITRITE OXIDIZER; NONHUMAN; OLIGONUCLEOTIDE PROBE; RESPIROMETRY; SLUDGE; STAINING; WASTE WATER MANAGEMENT;

COLONY COUNT, MICROBIAL; COMPUTER SIMULATION; GRAM-POSITIVE BACTERIA; IN SITU HYBRIDIZATION, FLUORESCENCE; MODELS, BIOLOGICAL; OXYGEN; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SEWAGE;

BACTERIA (MICROORGANISMS); NITROSOMONADALES;

EID: 35048876277     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21042     Document Type: Article

References (31)

1. Amann RI, Krumholz L, Stahl DA. 1990. Fluorescent oligonucleotide probing of whole cells for determinative, Phylogenetic and environmental studies in microbiology. J Bact 172:762-770.
2. Amann RI. 1995. In situ indentification of microorganisms by whole cell hybridization with rRNA-targeted nucleic acid probes. In: Akkeman ADC, van Elsas JD, de Bruigin FJ, editors. Molecular microbial ecology manual. Dortrecht: Kluwer Academic.
3. Amann RI, Ludwig W, Schleifer K-H. 1995. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev 59:143-169.
4. Andreottola G, Foladori P, Gelmini A, Ziglio G. 2001. Biomass active fraction evaluated by direct method and respirometric techniques. IWA conference on activated sludge population dynamics, Rome, 11-13 July, 273-279.
5. Beehary AO, Wentzel MC, Ekama GA. 2001. Evaluation of the batch test for measurement of active biomass in activated sludge mixed liquor. Res. Report W112, Department of Civil Engineering, University of Cape Town, Rondebosch 7700, South Africa.
6. Cronje GL, Beeharry AO, Wentzel MC, Ekama GA. 2002. Active biomass in activated sludge mixed liquor. Wat Res 36:439-444.
7. Daims H, Bruhl A, Amaan R, Schleifer K-H, Wagner M. 1999. The domain-specific probe EUB388 is insufficient for the detection of all bacteria: Development and evaluation of a more comprehensive probe set. System Appl Microbiol 22:434-444.
8. Daims H, Purkhold U, Bjerrum L, Arnold E, Wilderer PA, Wagner M. 2001. Nitrification in sequencing biofilm batch reactors: Lessons from molecular approaches. Wat Sci Tech 43(3):9-18.
9. Ekama GA, Dold PL, Marais GVR. 1986. Procedure for determining influent COD fractions and the maximum specific growth rate of heterotrophs in activated sludge. Wat Sci Tech 18:91-114.
10. Grady L, Diagger G. 1998. Biological wastewater treatment: Principles and practice. New York: Marcel Dekker, Inc.
11. Grady CPL, Smits BF, Barbeau DS. 1996. Variability in kinetic parameter estimates: A review of possible causes and proposed terminology. Wat Res 30(3):742-748.
12. Hicks RE, Amann RI, Stahl DA. 1992. Dual staining of natural bacterioplankton with 4′, 6-diamidino-2-phenylindole and fluorescent oligonucleotides probes targeting kingdom-level 16S rRNA sequences. Appl Environ Microbiol 58:2158-2163.
13. Holder-Snymann F, Ismail A, Mudaly DD, Bux F. 2004. Determination of the heterotrophic active bacteria in activated sludge using novel molecular techniques. Res. Report 1178/1/2004, Water Research Commision, P/Bag X03, Gezina 0031, South Africa.
14. Kappeler J, Gujer W. 1992. Estimation of kinetic parameters of heterotrophic biomass under aerobic conditions and characterization wastewater for activated sludge modeling. Wat Sci Tech 25(6):125-140.
15. Lee N, Nielsen PH, Andreasen KH, Juretschko S, Nielsen JL, Schleifer K-H, Wagner M. 1999. Combination of fluorescent in situ hybridization and Microautoradiography - a new tool for structure-function analyses in microbial ecology. Appl Environ Microbiol 65:1289-1297.
16. Lee BJ, Wentzel MC, Ekama GA. 2003. Batch test for the measurement of ordinary heterotrophic organism active mass in activated sludge mixed liquor. Res. Rept. W84, Department of Civil Eng., University of Cape Town, Rondebosch 7700, South Africa.
17. Manz W, Amann RI, Ludwig W, Schleifer K-H. 1992. Phylogenetic olygonucleotide probes for the major subclasses of proteobacteria: Problems and solutions. System Appl Microbiol 15:593-600.
18. Mobarry B, Wagner M, Urbain V, Rittmann B, Stahl DA. 1996. Phylogenetic probes for analysing abundance and spatial organisation of nitrifying bacteria. Appl Environ Microbiol 62(6):2156-2162.
19. Muyzer G, Waal EC, Uitterlinden AG. 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16s rRNA. Appl Environ Microbiol 59:695-700.
20. Oerther DB, de los Reyes FL, Raskin L. 1999. Interfacing phylogenetic oligonucleotide probe hybridization with representations of microbial populations and specific growth rates in mathematical models of activated sludge processes. Wat Sci Tech 39(1):11-20.
21. Porter KG, Feig Y. 1980. The use of DAPI for identifying and counting aquatic microflora. Limnol Oceanogr 25:943-948.
22. Schraam A, de beer D, van den Heuvel JC, Ottengraf S, Amaan R. 1999. Microscale distribution of populations and activities of Nitrosospira and Nitrospira spp. along a macroscale gradient in a nitrifying bioreactor: Quantification by in situ hybridization and the use of microsensors. Appl Environ Microbiol 65:3690-3696.
23. 1985. Standard Methods for the Examination of Water and Wastewater. 16th edn, American Public Health Assoc, 1015 15th Str. NW, Washington DC 20005, USA.
24. Ubisi MF, Jood TW, Wentzel MC, Ekama GA. 1997a. Activated sludge mixed liquor heterotrophic active biomass. Wat SA 23(3):239-248.
25. Ubisi MF, Wentzel MC, Ekama GA. 1997b. Organic and inorganic components of activated sludge mixed liquor. Res. Rept. W94, Dept. Civil Eng., Univer. of Cape, Ronderbosch 7700, South Africa.
26. Urbain V, Mobarry B, de Silva V, Stahl DA, Rittmann BE, Manem J. 1998. Integration of performance, molecular biology and modeling to describe the activated sludge process. Wat Sci Tech 37(4-5):223-229.
27. Wagner M, Rath G, Amann R, Koops H-P, Schleifer K-H. 1995. In situ identification of ammonia-oxidizing bacteria. System Appl Microbiol 18:251-264.
28. Wagner M, Rath G, Koops H-P, Flood J, Amann R. 1996. In situ analysis of nitrifying bacteria in sewage treatment plants. Wat Sci Tech 34:237-244.
29. Wentzel MC, Mbewe A, Ekama GA. 1995. Batch test for the measurements of readily biodegradable COD and active organisms concentrations in municipal wastewaters. Wat SA 21(2):117-124.
30. Wentzel MC, Ubisi MF, Ekama GA. 1998. Heterotrophic active biomass component of activated sludge mixed liquor. Wat Sci Tech 37(4-5): 79-87.
31. WRC. 1984. Theory, design and operation of nutrient removal activated sludge processes. Water research Commission, PO Box 824, Pretoria 0001, South Africa.