Functional analysis of the Mycoplasma genitalium MG312 protein reveals a specific requirement of the MG312 N-terminal domain for gliding motility

Journal of Bacteriology

Volumn 189, Issue 19, 2007, Pages 7014-7023

  Burgos, Raul ^a   Pich, Oscar Q ^a   Querol, Enrique ^a   Piñol, Jaume ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; BACTERIAL PROTEIN; GLYCINE; PROTEIN MG200; PROTEIN MG312; PROTEIN MG386; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIUM ADHERENCE; CARBOXY TERMINAL SEQUENCE; CELL MOTILITY; CELL STRUCTURE; CONTROLLED STUDY; DELETION MUTANT; HOMOLOGOUS RECOMBINATION; MYCOPLASMA GENITALIUM; NONHUMAN; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN LOCALIZATION;

BACTERIAL ADHESION; BACTERIAL PROTEINS; BLOTTING, WESTERN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENES, BACTERIAL; MICROSCOPY, ELECTRON, SCANNING; MODELS, GENETIC; MUTATION; MYCOPLASMA GENITALIUM; POLYMERASE CHAIN REACTION; PROTEIN BINDING;

MYCOPLASMA; MYCOPLASMA GENITALIUM; MYCOPLASMATALES;

EID: 34948904290     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.00975-07     Document Type: Article

References (45)

1. Balish, M. F., T. W. Hahn, P. L. Popham, and D. C. Krause. 2001. Stability of Mycoplasma pneumoniae cytadherence-accessory protein HMW1 correlates with its association with the triton shell. J. Bacteriol. 183:3680-3688.
2. Burgos, R., O. Q. Pich, M. Ferrer-Navarro, J. B. Baseman, E. Querol, and J. Pinol. 2006. Mycoplasma genitalium P140 and P110 cytadhesins are reciprocally stabilized and required for cell adhesion and terminal-organelle development. J. Bacteriol. 188:8627-8637.
3. Cohen, C. R., L. E. Manhart, E. A. Bukusi, S. Astete, R. C. Brunham, K. K. Holmes, S. K. Sinei, J. J. Bwayo, and P. A. Totten. 2002. Association between Mycoplasma genitalium and acute endometritis. Lancet 359:765-766.
4. Dhandayuthapani, S., M. W. Blaylock, C. M. Bebear, W. G. Rasmussen, and J. B. Baseman. 2001. Peptide methionine sulfoxide reductase (MsrA) is a virulence determinant in Mycoplasma genitalium. J. Bacteriol. 183:5645-5650.
5. Dhandayuthapani, S., W. G. Rasmussen, and J. B. Baseman. 1999. Disruption of gene mg218 of Mycoplasma genitalium through homologous recombination leads to an adherence-deficient phenotype. Proc. Natl. Acad. Sci. USA 96:5227-5232.
6. Dirksen, L. B., T. Proft, H. Hilbert, H. Plagens, R. Herrmann, and D. C. Krause. 1996. Sequence analysis and characterization of the hmw gene cluster of Mycoplasma pneumoniae. Gene 171:19-25.
7. Falk, L., H. Fredlund, and J. S. Jensen. 2005. Signs and symptoms of urethritis and cervicitis among women with or without Mycoplasma genitalium or Chlamydia trachomatis infection. Sex. Transm. Infect. 81:73-78.
8. Fraser, C. M., J. D. Gocayne, O. White, M. D. Adams, R. A. Clayton, R. D. Fleischmann, C. J. Bult, A. R. Kerlavage, G. Sutton, J. M. Kelley, R. D. Fritchman, J. F. Weidman, K. V. Small, M. Sandusky, J. Fuhrmann, D. Nguyen, T. R. Utterback, D. M. Saudek, C. A. Phillips, J. M. Merrick, J. F. Tomb, B. A. Dougherty, K. F. Bott, P. C. Hu, T. S. Lucier, S. N. Peterson, H. O. Smith, C. A. Hutchison III, and J. C. Venter. 1995. The minimal gene complement of Mycoplasma genitalium. Science 270:397-403.
9. Glass, J. I., N. Assad-Garcia, N. Alperovich, S. Yooseph, M. R. Lewis, M. Maruf, C. A. Hutchison III, H. O. Smith, and J. C. Venter. 2006. Essential genes of a minimal bacterium. Proc. Natl. Acad. Sci. USA 103:425-430.
10. Hahn, T. W., M. J. Willby, and D. C. Krause. 1998. HMW1 is required for cytadhesin P1 trafficking to the attachment organelle in Mycoplasma pneumoniae. J. Bacteriol. 180:1270-1276.
11. Harlow, E., and D. Lane. 1998. Antibodies: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
12. Hasselbring, B. M., J. L. Jordan, and D. C. Krause. 2005. Mutant analysis reveals a specific requirement for protein P30 in Mycoplasma pneumoniae gliding motility. J. Bacteriol. 187:6281-6289.
13. Hasselbring, B. M., and D. C. Krause. 2007. Cytoskeletal protein P41 is required to anchor the terminal organelle of the wall-less prokaryote Mycoplasma pneumoniae. Mol. Microbiol. 63:44-53.
14. Hasselbring, B. M., C. A. Page, E. S. Sheppard, and D. C. Krause. 2006. Transposon mutagenesis identifies genes associated with Mycoplasma pneumoniae gliding motility. J. Bacteriol. 188:6335-6345.
15. Hegermann, J., R. Herrmann, and F. Mayer. 2002. Cytoskeletal elements in the bacterium Mycoplasma pneumoniae. Naturwissenschaften 89:453-458.
16. Henderson, G. P., and G. J. Jensen. 2006. Three-dimensional structure of Mycoplasma pneumoniae's, attachment organelle and a model for its role in gliding motility. Mol. Microbiol. 60:376-385.
17. Himmelreich, R., H. Hilbert, H. Plagens, E. Pirkl, B. C. Li, and R. Herrmann. 1996. Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae. Nucleic Acids Res. 24:4420-4449.
18. Horner, P. J., C. B. Gilroy, B. J. Thomas, R. O. Naidoo, and D. Taylor-Robinson. 1993. Association of Mycoplasma genitalium with acute non-gonococcal urethritis. Lancet 342:582-585.
19. Jones, S., and J. M. Thornton. 1997. Analysis of protein-protein interaction sites using surface patches. J. Mol. Biol. 272:121-132.
20. Krause, D. C., and M. F. Balish. 2004. Cellular engineering in a minimal microbe: structure and assembly of the terminal organelle of Mycoplasma pneumoniae. Mol. Microbiol. 51:917-924.
21. Krause, D. C., and M. F. Balish. 2001. Structure, function, and assembly of the terminal organelle of Mycoplasma pneumoniae. FEMS Microbiol. Lett. 198:1-7.
22. Layh-Schmitt, G., H. Hilbert, and E. Pirkl. 1995. A spontaneous hemadsorption-negative mutant of Mycoplasma pneumoniae exhibits a truncated adhesin-related 30-kilodalton protein and lacks the cytadherence-accessory protein HMW1. J. Bacteriol. 177:843-846.
23. Manhart, L. E., C. W. Critchlow, K. K. Holmes, S. M. Dutro, D. A. Eschenbach, C. E. Stevens, and P. A. Totten. 2003. Mucopurulent cervicitis and Mycoplasma genitalium. J. Infect. Dis. 187:650-657.
24. Mernaugh, G. R., S. F. Dallo, S. C. Holt, and J. B. Baseman. 1993. Properties of adhering and nonadhering populations of Mycoplasma genitalium. Clin. Infect. Dis. 17(Suppl. 1):S69-S78.
25. Pich, O. Q., R. Burgos, M. Ferrer-Navarro, E. Querol, and J. Pinol. 2006. Mycoplasma genitalium mg200 and mg386 genes are involved in gliding motility but not in cytadherence. Mol. Microbiol. 60:1509-1519.
26. Pich, O. Q., R. Burgos, R. Planell, E. Querol, and J. Pinol. 2006. Comparative analysis of antibiotic resistance gene markers in Mycoplasma genitalium: application to studies of the minimal gene complement. Microbiology 152:519-527.
27. Popham, P. L., T. W. Hahn, K. A. Krebes, and D. C. Krause. 1997. Loss of HMW1 and HMW3 in noncytadhering mutants of Mycoplasma pneumoniae occurs post-translationally. Proc. Natl. Acad. Sci. USA 94:13979-13984.
28. Reddy, S. P., W. G. Rasmussen, and J. B. Baseman. 1996. Isolation and characterization of transposon Tn4001-generated, cytadherence-deficient transformants of Mycoplasma pneumoniae and Mycoplasma genitalium. FEMS Immunol. Med. Microbiol. 15:199-211.
29. Regula, J. T., G. Boguth, A. Gorg, J. Hegermann, F. Mayer, R. Frank, and R. Herrmann. 2001. Defining the mycoplasma 'cytoskeleton': the protein composition of the Triton X-100 insoluble fraction of the bacterium Mycoplasma pneumoniae determined by 2-D gel electrophoresis and mass spectrometry. Microbiology 147:1045-1057.
30. Roberts, R. J. 2004. Identifying protein function - a call for community action. PLoS Biol. 2:E42.
31. Robertson, J. A., and G. W. Stemke. 1995. Measurement of mollicute growth by ATP-dependent luminometry, p. 65-71. In S. Razin and J. G. Tully (ed.), Molecular and diagnostic procedures in mycoplasmology, vol. I. Academic Press, Inc., San Diego, CA.
32. Sambrook, J., and D. W. Russell. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
33. Seto, S., T. Kenri, T. Tomiyama, and M. Miyata. 2005. Involvement of P1 adhesin in gliding motility of Mycoplasma pneumoniae as revealed by the inhibitory effects of antibody under optimized gliding conditions. J. Bacteriol.187:1875-1877.
34. Seto, S., G. Layh-Schmitt, T. Kenri, and M. Miyata. 2001. Visualization of the attachment organelle and cytadherence proteins of Mycoplasma pneumoniae by immunofluorescence microscopy. J. Bacteriol. 183:1621-1630.
35. Seto, S., A. Uenoyama, and M. Miyata. 2005. Identification of a 521-kilodalton protein (Gli521) involved in force generation or force transmission tor Mycoplasma mobile gliding. J. Bacteriol. 187:3502-3510.
36. Seybert, A., R. Herrmann, and A. S. Frangakis. 2006. Structural analysis of Mycoplasma pneumoniae by cryo-electron tomography. J. Struct. Biol. 156:342-354.
37. Stevens, M. K., and D. C. Krause. 1991. Localization of the Mycoplasma pneumoniae cytadherence-accessory proteins HMW1 and HMW4 in the cytoskeletonlike Triton shell. J. Bacteriol. 173:1041-1050.
38. Stevens, M. K., and D. C. Krause. 1992. Mycoplasma pneumoniae cytadherence phase-variable protein HMW3 is a component of the attachment organelle. J. Bacteriol. 174:4265-4274.
39. Svenstrup, H. F., J. S. Jensen, K. Gevaert, S. Birkelund, and G. Christiansen. 2006. Identification and characterization of immunogenic proteins of Mycoplasma genitalium. Clin. Vaccine Immunol. 13:913-922.
40. Taylor-Robinson, D. 2002. Mycoplasma genitalium - an up-date. Int. J. STD AIDS 13:145-151.
41. Tully, J. G., D. L. Rose, R. F. Whitcomb, and R. P. Wenzel. 1979. Enhanced isolation of Mycoplasma pneumoniae from throat washings with a newly-modified culture medium. J. Infect. Dis. 139:478-482.
42. Uenoyama, A., A. Kusumoto, and M. Miyata. 2004. Identification of a 349-kilodalton protein (Gli349) responsible for cytadherence and glass binding during gliding of Mycoplasma mobile. J. Bacteriol. 186:1537-1545.
43. Uenoyama, A., and M. Miyata. 2005. Identification of a 123-kilodalton protein (Gli123) involved in machinery for gliding motility of Mycoplasma mobile. J. Bacteriol. 187:5578-5584.
44. Walker, J. E., M. Saraste, M. J. Runswick, and N. J. Gay. 1982. Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO J. 1:945-951.
45. Willby, M. J., M. F. Balish, S. M. Ross, K. K. Lee, J. L. Jordan, and D. C. Krause. 2004. HMW1 is required for stability and localization of HMW2 to the attachment organelle of Mycoplasma pneumoniae. J. Bacteriol. 186:8221-8228.