Novel staining-free proteomic method for simultaneous identification of proteins and determination of their pI values by using low-molecular-mass pI markers

Electrophoresis

Volumn 28, Issue 18, 2007, Pages 3315-3323

  Chmelík, Josef ^a   Mazanec, Karle ^a,b   Šlais, Karel ^a  

^a INSTITUTE OF ANALYTICAL CHEMISTRY   (Czech Republic)

^b BRNO UNIVERSITY OF TECHNOLOGY   (Czech Republic)

Author keywords

Determination of pI; Gel IEF; MALDI TOF; New staining free proteomic method; PI markers; TOF MS

Indexed keywords

MASS SPECTROMETRY; MOLECULAR MASS; PEPTIDES; SEARCH ENGINES;

DETERMINATION OF PI; GEL IEF; IDENTIFICATION OF PROTEINS; LOW MOLECULAR MASS; MALDI-TOF; NEW STAINING-FREE PROTEOMIC METHOD; PI MARKER; PI VALUES; PROTEOMICS METHODS; TOF MS;

GELS;

ALCOHOL; BETA AMYLASE; CHYMOTRYPSIN; TRYPSIN;

ACCURACY; ARTICLE; CONTROLLED STUDY; INTERMETHOD COMPARISON; ISOELECTRIC FOCUSING; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; PH; PROTEIN ANALYSIS; PROTEIN DATABASE; PROTEIN DETERMINATION; PROTEIN GLYCOSYLATION; PROTEIN PROCESSING; PROTEOMICS; SEPARATION TECHNIQUE;

EID: 34848927237     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.200600529     Document Type: Article

References (22)

1. Chiou, S. H., Wu, S. H., Anal. Chim. Acta 1999, 383, 47-60.
2. Svensson, H., Acta Chem. Scand. 1961, 15, 325-341.
3. Righetti, P. G., Castagna, A., Antonucci, F., Piubelli, C. et al., Clin. Chem. Lab. Med. 2003, 41, 425-438.
4. Righetti, P. G., Gianazza, E., Methods Biochem. Anal. 1987, 32, 215-278.
5. Chmelík J., J. Chromatogr. 1991, 545, 349-358.
6. Weber, G., Boček, P., Electrophoresis 1998, 19, 3094-3095.
7. Caslavska, J., Molteni, S., Chmelík, J., Šlais, K. et al., J. Chromatogr. 1994, 680, 549-559.
8. Shimura, K., Wang, Z., Matsumoto, H., Kasai, K., Electrophoresis 2000, 21, 603-610.
9. Righetti, P. G., Tudo, G., J. Chromatogr. 1981, 220, 115-194.
10. Slais, K., Friedl, Z., J. Chromatogr. A 1994, 661, 249-256.
11. Deml, M., Pospichal, J., Chmelík, J., J. Chromatogr. A 1995, 709, 39-49.
12. Schnabel, U., Grioss, F., Blaas, D., Kenndler, E., Anal. Chem. 1996, 68, 4300-4303.
13. Shimura, K., Zhi, W., Matsumoto, H., Kasai, K., Anal. Chem. 2000, 72, 4747-4757.
14. Štastná, M., Šlais, K., Electrophoresis 2005, 26, 3586-3591.
15. Crowley, T. A., Hayes, M. A., Proteomics 2005, 5, 3798-3804.
16. Salplachta, J., Marchetti, M., Chmelík, J., Allmaier, G., Rapid Commun. Mass Spectrom. 2005, 19, 2725-2728.
17. Vorm, O., Roepstorff, P., Mann, M., Anal. Chem. 1994, 66, 3281-3287.
18. Neuhoff, V., Stamm, R., Eibl, H., Electrophoresis 1985, 6, 427-448.
19. Shevchenko, A., Wilm, M., Vorm, O., Mann, M., Anal. Chem. 1996, 68, 850-858.
20. Rehulka, P., Popkov, A., Nadvornik, M., Planeta, J. et al., J. Mass Spectrom. 2006, 41, 448-453.
21. Mazanec, K., Šlais, K., Chmelík, J., J. Mass Spectrom. 2006, 41, 1570-1571.
22. Štastná, M., Šlais, K., Anal. Bioanal. Chem. 2005, 382, 65-72.