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Volumn 857, Issue 2, 2007, Pages 231-239
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Purification and characterization of RGD tumor-homing peptide conjugated human tumor necrosis factor α over-expressed in Escherichia coli
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Author keywords
Enterokinase; Fusion protein; Immobilized metal affinity chromatography; RGD peptide; TEV protease; Tumor necrosis factor
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Indexed keywords
ENTEROKINASE;
FUSION PROTEIN;
IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY;
RGD PEPTIDES;
CHARACTERIZATION;
CHROMATOGRAPHY;
ESCHERICHIA COLI;
PURIFICATION;
TOXICITY;
TUMORS;
PEPTIDES;
ALANYLCYSTEINYLASPARTYLCYSTEINYLARGINYLGLYCYLASPARTYLCYSTEINYL PHENYLALANYLCYSTEINYLGLYCINE;
ALPHAVBETA5 INTEGRIN;
AMINO ACID;
ARGINYLGLYCYLASPARTIC ACID;
HYBRID PROTEIN;
PEPTIDE;
POLYHISTIDINE;
TUMOR NECROSIS FACTOR ALPHA;
UNCLASSIFIED DRUG;
VITRONECTIN RECEPTOR;
ANIMAL CELL;
ANIMAL EXPERIMENT;
ANTINEOPLASTIC ACTIVITY;
ARTICLE;
BACTERIUM CULTURE;
BINDING AFFINITY;
CONTROLLED STUDY;
CYTOTOXICITY;
ESCHERICHIA COLI;
GEL FILTRATION;
HUMAN;
IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY;
MASS SPECTROMETRY;
NONHUMAN;
POLYACRYLAMIDE GEL ELECTROPHORESIS;
PRIORITY JOURNAL;
PROTEIN ANALYSIS;
PROTEIN BINDING;
PROTEIN DEGRADATION;
PROTEIN DEPLETION;
PROTEIN PURIFICATION;
PROTEIN SECRETION;
PROTEIN TARGETING;
TOBACCO VIRUS;
VIRUS;
WESTERN BLOTTING;
ANIMALS;
CELL ADHESION;
CHROMATOGRAPHY, AFFINITY;
ENDOPEPTIDASES;
ENTEROPEPTIDASE;
ESCHERICHIA COLI;
GENE EXPRESSION;
HISTIDINE;
HUMANS;
MICE;
NEOPLASMS;
OLIGOPEPTIDES;
RECOMBINANT FUSION PROTEINS;
SOLUBILITY;
SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;
TUMOR NECROSIS FACTOR-ALPHA;
ANIMALIA;
ESCHERICHIA COLI;
TOBACCO ETCH VIRUS;
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EID: 34548860361
PISSN: 15700232
EISSN: None
Source Type: Journal
DOI: 10.1016/j.jchromb.2007.07.022 Document Type: Article |
Times cited : (8)
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References (37)
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