Regulating gene expression in zebrafish embryos using light-activated, negatively charged peptide nucleic acids

Journal of the American Chemical Society

Volumn 129, Issue 36, 2007, Pages 11000-11001

  Tang, XinJing ^a   Maegawa, Shingo ^a   Weinberg, Eric S ^a   Dmochowski, Ivan J ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; MESSENGER RNA; PEPTIDE NUCLEIC ACID;

ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; DOWN REGULATION; EMBRYO; EMBRYO DEVELOPMENT; GENE EXPRESSION; HELA CELL; HYBRIDIZATION; IN VIVO STUDY; IRRADIATION; NONHUMAN; PHENOTYPE; PLASMID; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; SYNTHESIS; ZEBRA FISH;

ANIMALS; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GLYCOPROTEINS; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; LIGHT; PEPTIDE NUCLEIC ACIDS; RNA, MESSENGER; ZEBRAFISH;

EID: 34548756202     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja073723s     Document Type: Article

References (25)

1. (a) Summerton, J. Biochim. Biophys. Acta 1999, 1489, 141-158.
2. (b) Nasevicius, A.; Ekker, S. C. Nat. Genet. 2000, 26, 216-220.
3. (a) Hanvey, I. C.; Peffer, N. J.; Bisi, J. E.; Thomson, S. A.; Cadilla, R.; Josey, J. A.; Ricca, D. J.; Hassman, C. F.; Bonham, M. A.; Au, K. G.; Carter, S. G.; Bruckenstein, D. A.; Boyd, A. L.; Noble, S. A.; Babiss, L. E. Science 1992, 258, 1481-1485.
4. (b) Wickstrom, E.; Urtishak, K. A.; Choob, M.; Farber, S. A. Methods Cell Biol. 2004, 77, 137-158.
5. Urtishak, K. A.; Choob, M.; Tian, X.; Sternheim, N.; Talbot, W. S.; Wickstrom, E.; Farber, S. A. Dev. Dyn. 2003, 228, 405-413.
6. (a) Mayer, G.; Heckel, A. Angew. Chem., Int. Ed. 2006, 45, 4900-4921.
7. (b) Tang, X.; Dmochowski, I. J. Mol. BioSystems 2007, 3, 100-110.
8. (a) Tang, X.; Dmochowski, I. J. Org. Lett. 2005, 7, 279-282.
9. ( b) Ghosn, B.; Haselton, F. R.; Gee, K. R.; Monroe, W. T. Photochem. Photobiol. 2005, 81, 953-959.
10. (c) Kroeck, L.; Heckel, A. Angew. Chem., Int. Ed. 2005, 44, 471-473.
11. Monroe, W. T.; McQuain, M. M.; Chang, M. S.; Alexander, J. S.; Haselton, F. R. J. Biol. Chem. 1999, 274, 20895-20900.
12. Ando, H.; Furuta, T.; Tsien, R. Y.; Okamoto, H. Nat. Genet. 2001, 28, 317-325.
13. Ando, H.; Kobayashi, M.; Tsubokawa, T.; Uyemura, K.; Furuta, T.; Okamoto, H. Dev. Biol. 2005, 287, 456-468.
14. Shah, S.; Rangarajan, S.; Friedman, S. H. Angew. Chem., Int. Ed. 2005, 44, 1328-1332.
15. (a) Tang, X.; Richards, J. L.; Peritz, A. E.; Dmochowski, I. J. Bioorg. Med. Chem. Lett. 2005, 15, 5303-5306.
16. (b) Tang, X.; Dmochowski, I. J. Angew. Chem., Int. Ed. 2006, 45, 3523-3526.
17. Miller-Bertoglio, V. E.; Fisher, S.; Sáchez, A.; Mullins, M. C.; Halpern, M. E. Dev. Biol. 1997, 192, 537-550.
18. 2 at peak intensity, 365 nm.
19. Microinjection solutions were typically 0.5 mM, 10 nL per embryo, at the one- or two-cell stage. Embryos were cultured at 28°C.
20. Background activity was reduced by putting a 400 nm long-pass filter (Chroma) before the sample during microinjection. 2′-OMe-RNA served as an effective blocking strand, however, the analogous ncPNA-PL-sDNA conjugate was very toxic to developing zebrafish.
21. Schulte-Merker, S.; Lee, K. J.; McMahon, A. P.; Hammerschmidt, M. Nature 1997, 387, 862-863.
22. Fisher, S.; Amacher, S. L.; Halpern, M. E. Development 1997, 124, 1301-1311.
23. (a) Koos, D. S.; Ho, R. K. Dev. Biol. 1999, 215, 190-207.
24. (b) Fekany, K.; Yamanaka, Y.; Leung, T.; Sirotkin, H. I.; Topczewski, J.; Gates, M. A.; Hibi, M.; Renucci, A.; Stemple, D.; Radbill, A.; Schier, A. F.; Driever, W.; Hirano, T.; Talbot, W. S.; Solnica-Krezel, L. Development 1999, 126, 1427-1438.
25. Maegawa, S.; Varga, M.; Weinberg, E. S. Development 2006, 133, 3265-3276.