Photo-mediated gene activation using caged RNA/DNA in zebrafish embryos

Nature Genetics

Volumn 28, Issue 4, 2001, Pages 317-325

  Ando, Hideki ^a,b   Furuta, Toshiaki ^b,c   Tsien, Roger Y ^d   Okamoto, Hitoshi ^a,b  

^a INST OF PHYS AND CHEMICAL RESEARCH   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^c TOHO UNIVERSITY   (Japan)

^d HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTISENSE OLIGONUCLEOTIDE; COUMARIN DERIVATIVE; GREEN FLUORESCENT PROTEIN;

ANIMAL TISSUE; ARTICLE; EMBRYO; EMBRYO DEVELOPMENT; GENE ACTIVATION; GENE EXPRESSION; KNOCKOUT GENE; NONHUMAN; PHOTOACTIVATION; PHOTOLYSIS; PRIORITY JOURNAL; RNA TRANSLATION; ULTRAVIOLET RADIATION; ZEBRA FISH;

ANIMALS; AZO COMPOUNDS; COUMARINS; DNA; EMBRYO, NONMAMMALIAN; EYE ABNORMALITIES; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GREEN FLUORESCENT PROTEINS; HOMEODOMAIN PROTEINS; LUMINESCENT PROTEINS; MICROINJECTIONS; NERVE TISSUE PROTEINS; NERVOUS SYSTEM MALFORMATIONS; ORYZIAS; PHOTOLYSIS; PROSENCEPHALON; PROTEIN BIOSYNTHESIS; RNA STABILITY; RNA, MESSENGER; ULTRAVIOLET RAYS; ZEBRAFISH;

EID: 0034928842     PISSN: 10614036     EISSN: None     Source Type: Journal    
DOI: 10.1038/ng583     Document Type: Article

References (39)

1. Production of clones of homozygous diploid zebrafish (Brachydanio rerio)
2. Zebrafish: Genetic tools for studying vertebrate development
3. Acquisition of regional and cellular identities in the developing zebrafish nervous system
4. Patterning the brain of the zebrafish embryo
5. The syntenic relationship of the zebrafish and human genomes
6. Effective targeted gene 'knockdown' in zebrafish
7. Caged RNA: Photo-control of a ribozyme reaction
8. Targeting expression with light using caged DNA
9. Synthesis and properties of caged nucleotides
10. New caged groups: 7-Substituted coumarinylmethyl phosphate esters
11. Structure and early embryonic expression of the zebrafish engrailed-2 gene
12. Coordinate embryonic expression of three zebrafish engrailed genes
13. Expression of engrailed proteins in arthropods, annelids, and chordates
14. Expression pattern of zebrafish pax genes suggests a role in early brain regionalization
15. Zebrafish pax[zf-a]: A paired box-containing gene expressed in the neural tube
16. Cloning of the zebrafish krox-20 gene (krx-20) and its expression during hindbrain development
17. Differential expression of two zebrafish emx homeoprotein mRNAs in the developing brain
18. Different spatio-temporal expressions of three otx homeoprotein transcripts during zebrafish embryogenesis
19. Fgf8 is mutated in zebrafish acerebellar (ace) mutants and is required for maintenance of midbrain-hindbrain boundary development and somitogenesis
20. High-frequency generation of transgenic zebrafish which reliably express GFP in whole muscles or the whole body by using promoters of zebrafish origin
21. The midbrain-hindbrain boundary genetic cascade is activated ectopically in the diencephalon in response to the widespread expression of one of its components, the medaka gene Ol-eng2
22. Engrailed. Wnt and Pax genes regulate midbrain-hindbrain development
23. Engrailed-1 as a target of the Wnt-1 signalling pathway in vertebrate midbrain development
24. Ocular and cerebellar defects in zebrafish induced by overexpression of the LIM domains of the Islet-3 LIM/homeodomain protein
25. Engrailed defines the position of dorsal dimesenchephalic boundary by repressing diencephalic fate
26. Use of the Gal4-UAS technique for targeted gene expression in the zebrafish
27. Drosophila mitotic domain boundaries as cell fate boundaries
28. Light-activated gene expression
29. Brominated 7-hydroxycoumarin-4-ylmethyls: Photolabile protecting groups with biologically useful cross-sections for two photon photolysis
30. Studies on stable diazoalkanes as potential fluorogenic reagents I. 7-Substituted 4-diazomethylcoumarins
31. Visualization of cranial motor neurons in live transgenic zebrafish expressing green fluorescent protein under the control of the Islet-1 promoter/enhancer
32. Functional repression of Islet-2 by disruption of complex with Ldb impairs peripheral axonal outgrowth in embryonic zebrafish
33. Use of a recombinant retrovirus to study post-implantation cell lineage in mouse embryos
34. Organization of hindbrain segments in the zebrafish embryo