A microarray-based protocol for monitoring the growth of yeast overexpression strains

Nature Protocols

Volumn 1, Issue 2, 2006, Pages 569-576

  Butcher, Rebecca A ^a,b,c   Schreiber, Stuart L ^a,b  

^a HARVARD UNIVERSITY   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^c HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GALACTOSE;

ARTICLE; DRUG EFFECT; GENE EXPRESSION REGULATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; METHODOLOGY; PROTEIN MICROARRAY; SACCHAROMYCES CEREVISIAE;

GALACTOSE; GENE EXPRESSION REGULATION, FUNGAL; PROTEIN ARRAY ANALYSIS; SACCHAROMYCES CEREVISIAE;

EID: 34548723373     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2006.80     Document Type: Article

References (11)

1. Giaever, G. et al. Functional profiling of the Saccharomyces cerevisiae genome. Nature 418, 387-391 (2002).
2. Winzeter, E.A. et al. Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science 285, 901-906 (1999).
3. Glaever, G. et al. Genomic profiling of drug sensitivities via induced haploinsufficiency. Nature Genet. 21, 278-283 (1999).
4. Lum, P.Y. et al. Discovering modes of action for therapeutic compounds using a genome-wide screen of yeast heterozygotes. Cell 116, 121-137 (2004).
5. Giaever, G. et al. Chemagenomic profiling: Identifying the functional interactions of small molecules in yeast. Proc. Natl. Acad. Sci. USA 101, 793-798 (2004).
6. Parsons, A.B. et al. Integration of chemical-genetic and genetic interaction data links bioactive compounds to cellular target pathways. Nature Biotechnol. 22, 62-69 (2004).
7. Butcher, R.A. et al. Microarray-based method for monitoring yeast overexpression strains reveals small-molecule targets in TOR pathway. Nature Chem. Biol. 2, 103-109 (2006).
8. Schiestl, R.H. & Gietz, R.D. High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier. Curr. Genet. 16, 339-346 (1989).
9. Soni, R., Carmichael, J.P. & Murray, J.A. Parameters affecting lithium acetate-mediated transformation of Saccharomyces cerevisiae and development of a rapid and simplified procedure. Curr. Genet. 24, 455-459 (1993).
10. Gollub, J. et al. The Stanford Microarray Database: Data access and quality assessment tools. Nucleic Acids Res. 31, 94-96 (2003).
11. Heitman, J., Movva, N.R. & Hall, M.N. Targets for cell cycle arrest by the immunosuppressant rapamycin in yeast. Science 253, 905-909 (1991).