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Volumn 1, Issue 2, 2006, Pages 1022-1028

A double-reporter splicing assay for determining splicing efficiency in mammalian cells

Author keywords

[No Author keywords available]

Indexed keywords

ALTERNATIVE RNA SPLICING; ANIMAL; ARTICLE; CELL LINE; EPITHELIUM CELL; GENETIC PROCEDURES; GENETICS; HUMAN; MAMMAL; METABOLISM; PHYSIOLOGY; REPORTER GENE; RNA SPLICING;

EID: 34548707119     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2006.148     Document Type: Article
Times cited : (34)

References (14)
  • 1
    • 0347623371 scopus 로고    scopus 로고
    • Genome-wide survey of human alternative pre-mRNA splicing with exon junction microarrays
    • Johnson, J.M. et al. Genome-wide survey of human alternative pre-mRNA splicing with exon junction microarrays. Science 302 2141-2144 (2003).
    • (2003) Science , vol.302 , pp. 2141-2144
    • Johnson, J.M.1
  • 2
    • 0036534129 scopus 로고    scopus 로고
    • Alternative splicing: Multiple control mechanisms and involvement in human disease
    • Caceres, J.F. & Kornblihtt, A.R. Alternative splicing: Multiple control mechanisms and involvement in human disease. Trends Genet. 18 186-193 (2002).
    • (2002) Trends Genet , vol.18 , pp. 186-193
    • Caceres, J.F.1    Kornblihtt, A.R.2
  • 3
    • 0036207384 scopus 로고    scopus 로고
    • Listening to silence and understanding nonsense: Exonic mutations that affect splicing
    • Cartegni, L., Chew, S.L. & Krainer, A.R. Listening to silence and understanding nonsense: Exonic mutations that affect splicing. Nat. Rev. Genet. 3, 285-298 (2002).
    • (2002) Nat. Rev. Genet , vol.3 , pp. 285-298
    • Cartegni, L.1    Chew, S.L.2    Krainer, A.R.3
  • 4
    • 0035158371 scopus 로고    scopus 로고
    • in vivo reporter system for measuring increased inclusion of exon 7 in SMN2 mRNA: Potential therapy of SMA
    • Zhang, M.L., Lorson, C.L., Androphy, E.J. & Zhou, J. An in vivo reporter system for measuring increased inclusion of exon 7 in SMN2 mRNA: Potential therapy of SMA. Gene Ther. 8, 1532-1538 (2001).
    • (2001) Gene Ther , vol.8 , pp. 1532-1538
    • Zhang, M.L.1    Lorson, C.L.2    Androphy, E.J.3    Zhou, J.A.4
  • 5
    • 31544458695 scopus 로고    scopus 로고
    • Endosome trapping limits the efficiency of splicing correction by PNA-oligolysine conjugates
    • Abes, S. et al. Endosome trapping limits the efficiency of splicing correction by PNA-oligolysine conjugates. J. Control. Release 110, 595-604 (2006).
    • (2006) J. Control. Release , vol.110 , pp. 595-604
    • Abes, S.1
  • 6
    • 0034685821 scopus 로고    scopus 로고
    • Eukaryotic selenocysteine incorporation follows a nonprocessive mechanism that competes with translational termination
    • Nasim, M.T. et al. Eukaryotic selenocysteine incorporation follows a nonprocessive mechanism that competes with translational termination. J. Biol. Chem. 275, 14846-14852 (2000).
    • (2000) J. Biol. Chem , vol.275 , pp. 14846-14852
    • Nasim, M.T.1
  • 7
    • 17144470484 scopus 로고    scopus 로고
    • A double reporter assay for detecting changes in the ratio of spliced and unspliced mRNA in mammalian cells
    • Nasim, M.T., Chowdhury, H.M. & Eperon, I.C. A double reporter assay for detecting changes in the ratio of spliced and unspliced mRNA in mammalian cells. Nucleic Acids Res. 30, e109 (2002).
    • (2002) Nucleic Acids Res , vol.30
    • Nasim, M.T.1    Chowdhury, H.M.2    Eperon, I.C.3
  • 8
    • 0001828219 scopus 로고    scopus 로고
    • UAG readthrough in mammalian cells: Effect of upstream and downstream stop codon contexts reveal different signals
    • Cassan, M. & Rousset, J.P. UAG readthrough in mammalian cells: Effect of upstream and downstream stop codon contexts reveal different signals. BMC Mol. Biol. 2, 3 (2001).
    • (2001) BMC Mol. Biol , vol.2 , pp. 3
    • Cassan, M.1    Rousset, J.P.2
  • 9
    • 13444249748 scopus 로고    scopus 로고
    • Activation of pre-mRNA splicing by human RNPS1 is regulated by CK2 phosphorylation
    • Trembley, J.H. et al. Activation of pre-mRNA splicing by human RNPS1 is regulated by CK2 phosphorylation. Mol. Cell. Biol. 25 1446-1457 (2005).
    • (2005) Mol. Cell. Biol , vol.25 , pp. 1446-1457
    • Trembley, J.H.1
  • 10
    • 0025098474 scopus 로고
    • Exon definition may facilitate splice site selection in RNAs with multiple exons
    • Robberson, B.L., Cote, G.J. & Berget, S.M. Exon definition may facilitate splice site selection in RNAs with multiple exons. Mol. Cell. Biol. 10, 84-94 (1990).
    • (1990) Mol. Cell. Biol , vol.10 , pp. 84-94
    • Robberson, B.L.1    Cote, G.J.2    Berget, S.M.3
  • 11
    • 0037669004 scopus 로고    scopus 로고
    • HnRNP G and Tra2β: Opposite effects on splicing matched by antagonism in RNA binding
    • Nasim, M.T., Chemova, T.K., Chowdhury, H.M., Yue, B.G. & Eperon, I.C. HnRNP G and Tra2β: Opposite effects on splicing matched by antagonism in RNA binding. Hum. Mol. Genet. 12, 1337-1348 (2003).
    • (2003) Hum. Mol. Genet , vol.12 , pp. 1337-1348
    • Nasim, M.T.1    Chemova, T.K.2    Chowdhury, H.M.3    Yue, B.G.4    Eperon, I.C.5
  • 12
    • 0942276839 scopus 로고    scopus 로고
    • The intronic prothrombin 19911A>G polymorphism influences splicing efficiency and modulates effects of the 20210G>A polymorphism on mRNA amount and expression in a stable reporter gene assay system
    • von Ahsen, N. & Oellerich, M. The intronic prothrombin 19911A>G polymorphism influences splicing efficiency and modulates effects of the 20210G>A polymorphism on mRNA amount and expression in a stable reporter gene assay system. Blood 103, 586-593 (2004).
    • (2004) Blood , vol.103 , pp. 586-593
    • von Ahsen, N.1    Oellerich, M.2
  • 13
    • 23844448397 scopus 로고    scopus 로고
    • ATPase/helicase activities of p68 RNA helicase are required for pre-mRNA splicing but not for assembly of the spliceosome
    • Lin, C., Yang, L., Yang, J.J., Huang, Y. & Liu, Z.R. ATPase/helicase activities of p68 RNA helicase are required for pre-mRNA splicing but not for assembly of the spliceosome. Mol. Cell. Biol. 25, 7484-7493 (2005).
    • (2005) Mol. Cell. Biol , vol.25 , pp. 7484-7493
    • Lin, C.1    Yang, L.2    Yang, J.J.3    Huang, Y.4    Liu, Z.R.5
  • 14
    • 0029916386 scopus 로고    scopus 로고
    • Analysis of eukaryotic mRNA structures directing cotranslational incorporation of selenocysteine
    • Kollmus, H., Flohe, L. & McCarthy, J.E. Analysis of eukaryotic mRNA structures directing cotranslational incorporation of selenocysteine. Nucleic Acids Res. 24, 1195-1201 (1996).
    • (1996) Nucleic Acids Res , vol.24 , pp. 1195-1201
    • Kollmus, H.1    Flohe, L.2    McCarthy, J.E.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.