The lipopolysaccharide-recognition mechanism in cells expressing TLR4 and CD14 but lacking MD-2

FEMS Immunology and Medical Microbiology

Volumn 51, Issue 1, 2007, Pages 84-91

  Ohnishi, Takahiro ^a   Muroi, Masashi ^a   Tanamoto, Ken Ichi ^a  

^a NATIONAL INSTITUTE OF HEALTH SCIENCES   (Japan)

Author keywords

Cell activation; LPS recognition; Toll like receptor signal transduction

Indexed keywords

CD14 ANTIGEN; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; LIPOPOLYSACCHARIDE; PROTEIN MD 2; TOLL LIKE RECEPTOR 4;

ARTICLE; CELL SURFACE; EPITHELIUM CELL; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN BINDING; SIGNAL TRANSDUCTION;

ACUTE-PHASE PROTEINS; ANTIGENS, CD14; CARRIER PROTEINS; CELL LINE; HUMANS; LIPOPOLYSACCHARIDES; LYMPHOCYTE ANTIGEN 96; MEMBRANE GLYCOPROTEINS; SIGNAL TRANSDUCTION; TOLL-LIKE RECEPTOR 4;

EID: 34548587922     PISSN: 09288244     EISSN: 1574695X     Source Type: Journal    
DOI: 10.1111/j.1574-695X.2007.00281.x     Document Type: Article

References (24)

1. da Silva Correia J Ulevitch RJ (2002) MD-2 and TLR4 N-linked glycosylations are important for a functional lipopolysaccharide receptor. J Biol Chem 277 : 1845 1854.
2. da Silva Correia J, Soldau K, Christen U, Tobias PS Ulevitch RJ (2001) Lipopolysaccharide is in close proximity to each of the proteins in its membrane receptor complex. Transfer from CD14 to TLR4 and MD-2. J Biol Chem 276 : 21129 21135.
3. Gioannini TL, Teghanemt A, Zhang D, Coussens NP, Dockstader W, Ramaswamy S Weiss JP (2004) Isolation of an endotoxin-MD-2 complex that produces Toll-like receptor 4-dependent cell activation at picomolar concentrations. Proc Natl Acad Sci USA 101 : 4186 4191.
4. Hatada EN, Krappmann D Scheidereit C (2000) NF-kappaB and the innate immune response. Curr Opin Immunol 12 : 52 58.
5. Hirschfeld M, Ma Y, Weis JH, Vogel SN Weis JJ (2000) Cutting edge: repurification of lipopolysaccharide eliminates signaling through both human and murine toll-like receptor 2. J Immunol 165 : 618 622.
6. Husebye H, Halaas O, Stenmark H, Tunheim G, Sandanger O, Bogen B, Brech A, Latz E Espevik T (2006) Endocytic pathways regulate Toll-like receptor 4 signaling and link innate and adaptive immunity. EMBO J 25 : 683 692.
7. Jia HP, Kline JN, Penisten A, Apicella MA, Gioannini TL, Weiss J McCray PB Jr. (2004) Endotoxin responsiveness of human airway epithelia is limited by low expression of MD-2. Am J Physiol Lung Cell Mol Physiol 287 : L428 L437.
8. Jiang Q, Akashi S, Miyake K Petty HR (2000) Lipopolysaccharide induces physical proximity between CD14 and toll-like receptor 4 (TLR4) prior to nuclear translocation of NF-kappa B. J Immunol 165 : 3541 3544.
9. Kawai T, Adachi O, Ogawa T, Takeda K Akira S (1999) Unresponsiveness of MyD88-deficient mice to endotoxin. Immunity 11 : 115 122.
10. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 : 680 685.
11. Landmann R, Zimmerli W, Sansano S, Link S, Hahn A, Glauser MP Calandra T (1995) Increased circulating soluble CD14 is associated with high mortality in gram-negative septic shock. J Infect Dis 171 : 639 644.
12. Landmann R, Reber AM, Sansano S Zimmerli W (1996) Function of soluble CD14 in serum from patients with septic shock. J Infect Dis 173 : 661 668.
13. Muroi M, Ohnishi T Tanamoto K (2002) MD-2, a novel accessory molecule, is involved in species-specific actions of Salmonella lipid A. Infect Immun 70 : 3546 3550.
14. Nagai Y, Akashi S, Nagafuku M, Ogata M, Iwakura Y, Akira S, Kitamura T, Kosugi A, Kimoto M Miyake K (2002) Essential role of MD-2 in LPS responsiveness and TLR4 distribution. Nat Immunol 3 : 667 672.
15. Ohnishi T, Muroi M Tanamoto K (2001) N-linked glycosylations at Asn(26) and Asn(114) of human MD-2 are required for toll-like receptor 4-mediated activation of NF-kappaB by lipopolysaccharide. J Immunol 167 : 3354 3359.
16. Ohnishi T, Muroi M Tanamoto K (2003) MD-2 is necessary for the toll-like receptor 4 protein to undergo glycosylation essential for its translocation to the cell surface. Clin Diagn Lab Immunol 10 : 405 410.
17. Pugin J, Stern-Voeffray S, Daubeuf B, Matthay MA, Elson G Dunn-Siegrist I (2004) Soluble MD-2 activity in plasma from patients with severe sepsis and septic shock. Blood 104 : 4071 4079.
18. Saitoh S, Akashi S, Yamada T et al 2004) Lipid A antagonist, lipid IVa, is distinct from lipid A in interaction with Toll-like receptor 4 (TLR4)-MD-2 and ligand-induced TLR4 oligomerization. Int Immunol 16 : 961 969.
19. Sakaguchi S, Negishi H, Asagiri M, Nakajima C, Mizutani T, Takaoka A, Honda K Taniguchi T (2003) Essential role of IRF-3 in lipopolysaccharide-induced interferon-beta gene expression and endotoxin shock. Biochem Biophys Res Commun 306 : 860 866.
20. Schletter J, Heine H, Ulmer AJ Rietschel ET (1995) Molecular mechanisms of endotoxin activity. Arch Microbiol 164 : 383 389.
21. Schromm AB, Lien E, Henneke P et al 2001) Molecular genetic analysis of an endotoxin nonresponder mutant cell line: a point mutation in a conserved region of MD-2 abolishes endotoxin-induced signaling. J Exp Med 194 : 79 88.
22. Van Amersfoort ES, Van Berkel TJ Kuiper J (2003) Receptors, mediators, and mechanisms involved in bacterial sepsis and septic shock. Clin Microbiol Rev 16 : 379 414.
23. Viriyakosol S, Tobias PS, Kitchens RL Kirkland TN (2001) MD-2 binds to bacterial lipopolysaccharide. J Biol Chem 276 : 38044 38051.
24. Visintin A, Mazzoni A, Spitzer JA Segal DM (2001) Secreted MD-2 is a large polymeric protein that efficiently confers lipopolysaccharide sensitivity to Toll-like receptor 4. Proc Natl Acad Sci USA 98 : 12156 12161.