Eugenol Modulates Cyclooxygenase-2 Expression Through the Activation of Nuclear Factor Kappa B in Human Osteoblasts

Journal of Endodontics

Volumn 33, Issue 10, 2007, Pages 1177-1182

  Lee, Ya Yun ^a   Yang, Shue Fen ^a,b   Ho, Weng Hang ^a   Lee, Yuan Ho ^c   Hung, Shan Ling ^a  

^a NATIONAL YANG MING UNIVERSITY   (Taiwan)

^b TAIPEI VETERANS GENERAL HOSPITAL   (Taiwan)

^c FO GUANG UNIVERSITY   (Taiwan)

Author keywords

Cyclooxygenase 2; cytotoxicity; eugenol; nuclear factor kappa B; osteoblasts

Indexed keywords

ACETYLCYSTEINE; CYCLOOXYGENASE 2; CYCLOOXYGENASE 2 INHIBITOR; EUGENOL; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; ROOT CANAL FILLING MATERIAL; SCAVENGER;

ARTICLE; CELL LINE; CELL NUCLEUS; CELL SHAPE; CELL SURVIVAL; CONFOCAL MICROSCOPY; CYTOPLASM; DOSE RESPONSE; DRUG ANTAGONISM; DRUG EFFECT; ENZYMOLOGY; FLUORESCENT ANTIBODY TECHNIQUE; HUMAN; OSTEOBLAST; WESTERN BLOTTING;

ACETYLCYSTEINE; BLOTTING, WESTERN; CELL LINE; CELL NUCLEUS; CELL SHAPE; CELL SURVIVAL; CYCLOOXYGENASE 2; CYCLOOXYGENASE 2 INHIBITORS; CYTOPLASM; DOSE-RESPONSE RELATIONSHIP, DRUG; EUGENOL; FLUORESCENT ANTIBODY TECHNIQUE, DIRECT; FREE RADICAL SCAVENGERS; HUMANS; MICROSCOPY, CONFOCAL; NF-KAPPA B; OSTEOBLASTS; ROOT CANAL FILLING MATERIALS;

EID: 34548529335     PISSN: 00992399     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.joen.2007.05.011     Document Type: Article

References (35)

1. Kolokouris I., Economides N., Beltes P., and Vlemmas I. In vivo comparison of the biocompatibility of two root canal sealers implanted into the subcutaneous connective tissue of rats. J Endod 24 (1998) 82-85
2. Sousa C.J., Loyola A.M., Versiani M.A., et al. A comparative histological evaluation of the biocompatibility of materials used in apical surgery. Int Endod J 37 (2004) 738-748
3. Hume W.R. An analysis of the release and the diffusion through dentin of eugenol from zinc oxide-eugenol mixtures. J Dent Res 63 (1984) 881-884
4. Ho Y.C., Huang F.M., and Chang Y.C. Mechanisms of cytotoxicity of eugenol in human osteoblastic cells in vitro. Int Endod J 39 (2006) 389-393
5. Hayden M.S., West A.P., and Ghosh S. NF-kappaB and the immune response. Oncogene 25 (2006) 6758-6780
6. Vermes C., Roebuck K.A., Chandrasekaran R., et al. Particulate wear debris activates protein tyrosine kinases and nuclear factor kappaB, which down-regulates type I collagen synthesis in human osteoblasts. J Bone Miner Res 15 (2000) 1756-1765
7. Boyce B.F., Xing L., Franzoso G., and Siebenlist U. Required and nonessential functions of nuclear factor-kappa B in bone cells. Bone 25 (1999) 137-139
8. Raisz L.G. Physiologic and pathologic roles of prostaglandins and other eicosanoids in bone metabolism. J Nutr 125 (1995) 2024S-2027S
9. Needleman P., Turk J., Jakschik B.A., et al. Arachidonic acid metabolism. Annu Rev Biochem 55 (1986) 69-102
10. Coon D., Gulati A., Cowan C., and He J. The role of cyclooxygenase-2 (COX-2) in inflammatory bone resorption. J Endod 33 (2007) 432-436
11. Miyauchi M., Hiraoka M., Oka H., et al. Immuno-localization of COX-1 and COX-2 in the rat molar periodontal tissue after topical application of lipopolysaccharide. Arch Oral Biol 49 (2004) 739-746
12. Anderson G.D., Hauser S.D., McGarity K.L., et al. Selective inhibition of cyclooxygenase (COX)-2 reverses inflammation and expression of COX-2 and interleukin 6 in rat adjuvant arthritis. J Clin Invest 97 (1996) 2672-2679
13. Orstavik D. Materials used for root canal obtruation: technical, biological and clinical testing. Endod Top 12 (2005) 25-38
14. Zhang X., Morham S.G., Langenbach R., et al. Evidence for a direct role of cyclooxygenase 2 in implant wear debris-induced osteolysis. J Bone Miner Res 16 (2001) 660-670
15. Wu K.K. Control of cyclooxygenase-2 transcriptional activation by pro-inflammatory mediators. Prostaglandins Leukot Essent Fatty Acids 72 (2005) 89-93
16. Ling L.J., Ho F.C., Chen Y.T., et al. Areca nut extracts modulated expression of alkaline phosphatase and receptor activator of nuclear factor kappaB ligand in osteoblasts. J Clin Periodontol 32 (2005) 353-359
17. Origuchi T., Migita K., Nakashima T., et al. Regulation of cyclooxygenase-2 expression in human osteoblastic cells by N-acetylcysteine. J Lab Clin Med 136 (2000) 390-394
18. Zafarullah M., Li W.Q., Sylvester J., and Ahmad M. Molecular mechanisms of N-acetylcysteine actions. Cell Mol Life Sci 60 (2003) 6-20
19. Silva T.A., Rosa A.L., and Lara V.S. Dentin matrix proteins and soluble factors: intrinsic regulatory signals for healing and resorption of dental and periodontal tissues?. Oral Dis 10 (2004) 63-74
20. Abou H., Camps J., Dejou J., and Franquin J.C. Eugenol diffusion through dentin related to dentin hydraulic conductance. Dent Mater 14 (1998) 229-236
21. 2) production by arachidonic acid, estrogen and parathyroid hormone in MG-63 and MC3T3-E1 osteoblast-like cells. Prostaglandins Leukot Essent Fatty Acids 73 (2005) 423-430
22. Fujisawa S., Atsumi T., Kadoma Y., and Sakagami H. Antioxidant and prooxidant action of eugenol-related compounds and their cytotoxicity. Toxicology 177 (2002) 39-54
23. Thompson D., Norbeck K., Olsson L.I., et al. Peroxidase-catalyzed oxidation of eugenol: formation of a cytotoxic metabolite(s). J Biol Chem 264 (1989) 1016-1021
24. Yoo C.B., Han K.T., Cho K.S., et al. Eugenol isolated from the essential oil of Eugenia caryophyllata induces a reactive oxygen species-mediated apoptosis in HL-60 human promyelocytic leukemia cells. Cancer Lett 225 (2005) 41-52
25. Allen R.G., and Tresini M. Oxidative stress and gene regulation. Free Radic Biol Med 28 (2000) 463-499
26. Ambili R., Santhi W.S., Janam P., et al. Expression of activated transcription factor nuclear factor-kappaB in periodontally diseased tissues. J Periodontol 76 (2005) 1148-1153
27. Mercurio F., and Manning A.M. NF-kappaB as a primary regulator of the stress response. Oncogene 18 (1999) 6163-6171
28. Fritz E.A., Jacobs J.J., Glant T.T., and Roebuck K.A. Chemokine IL-8 induction by particulate wear debris in osteoblasts is mediated by NF-kappaB. J Orthop Res 23 (2005) 1249-1257
29. Raghavenra H., Diwakr B.T., Lokesh B.R., and Naidu K.A. Eugenol-the active principle from cloves inhibits 5-lipoxygenase activity and leukotriene-C4 in human PMNL cells. Prostaglandins Leukot Essent Fatty Acids 74 (2006) 23-27
30. Kim S.S., Oh O.J., Min H.Y., et al. Eugenol suppresses cyclooxygenase-2 expression in lipopolysaccharide-stimulated mouse macrophage RAW264.7 cells. Life Sci 73 (2003) 337-348
31. Baek S.H., Plenk Jr. H., and Kim S. Periapical tissue responses and cementum regeneration with amalgam, SuperEBA, and MTA as root-end filling materials. J Endod 31 (2005) 444-449
32. Huang F.M., Chou L.S., Chou M.Y., and Chang Y.C. Protective effect of NAC on formaldehyde-containing-ZOE-based root-canal-sealers-induced cyclooxygenase-2 expression and cytotoxicity in human osteoblastic cells. J Biomed Mater Res Part B Appl Biomater 74 (2005) 768-773
33. Allan N.A., Walton R.C., and Schaeffer M.A. Setting times for endodontic sealers under clinical usage and in vitro conditions. J Endod 27 (2001) 421-423
34. Boyan B.D., Hummert T.W., Dean D.D., and Schwartz Z. Role of material surfaces in regulating bone and cartilage cell response. Biomaterials 17 (1996) 137-146
35. Morissette G., Moreau E., Gaudreault R., and Marceau F. Massive cell vacuolization induced by organic amines such as procainamide. J Pharmacol Exp Ther 310 (2004) 395-406