Detection of Phytophthora nicotianae and P. palmivora in citrus roots using PCR-RFLP in comparison with other methods

European Journal of Plant Pathology

Volumn 119, Issue 2, 2007, Pages 143-158

  Bowman, Kim D ^a   Albrecht, Ute ^a   Graham, James H ^b   Bright, Diane B ^b  

^a U S HORTICULTURAL RESEARCH LABORATORY   (United States)

^b UNIVERSITY OF FLORIDA   (United States)

Author keywords

ITS regions; Phytophthora root rot

Indexed keywords

ANGIOSPERM; COMPARATIVE STUDY; DETECTION METHOD; FRUIT; PATHOGEN; POLYMERASE CHAIN REACTION; POLYMORPHISM; ROOT; ROOT ROT;

CITRUS; PHYTOPHTHORA; PHYTOPHTHORA NICOTIANAE; PHYTOPHTHORA PALMIVORA;

EID: 34548512027     PISSN: 09291873     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10658-007-9135-7     Document Type: Article

References (47)

1. Albrecht, U., & Bowman, K. D. (2004). Growth comparison of citrus roostocks after artificial infection with Phytophthora. Proceedings of the Florida State Horticultural Society, 117, 156-160.
2. Böhm, J., Hahn, A., Schubert, R., Bahnweg, G., Adler, N., Nechwatal, J., Oehlmann, R., & Oßwald, W. (1999). Real-time quantitative PCR: DNA determination in isolated spores of the mycorrhizal fungus Glomus mossea and monitoring of Phytophthora infestans and Phytophthora citricola in their respective host plants. Journal of Phytopathology, 147, 409-416.
3. Bonants, P., Hagenaar-de Weerdt, M., van Gent-Pelzer, M., Lacourt, I., Cooke, D., & Duncan, J. (1997). Detection and identification of Phytophthora fragariae Hickman by the polymerase chain reaction. European Journal of Plant Pathology, 103, 345-355.
4. Bonants, P. J. M., Hagenaar-de Weerdt, M., Man in't Veld, W. A., & Baayen, R. P. (2000). Molecular characterization of natural hybrids of Phytophthora nicotianae and P. cactorum. Phytopathology, 90, 867-874.
5. Bowman, K. D., Shapiro, J. P., & Lapointe, S. L. (2001). Sources of resistance to Diaprepes weevil in subfamily Aurantiodeae, Rutaceae. HortScience, 36, 332-336.
6. Bowman, K. D., Albano, J. P., & Graham, J. H. (2002). Greenhouse testing of rootstocks for resistance to Phytophthora species in flatwoods soil. Proceedings of the Florida State Horticultural Society, 115, 10-13.
7. Bowman, K. D., Graham, J. H., & Adair, R. C. (2003). Young tree growth in a flatwoods rootstock trial with Diaprepes weevil and Phytophthora diseases. Proceedings of the Florida State Horticultural Society, 116, 249-251.
8. Cavalier-Smith, T. A. (1986). The Kingdom Chromista: Origin and systematics. In: F. E. Round & D. J. Chapman (Eds.), Progress on phycological research, (Vol. 4, pp. 309-347). Bristol: Biopress.
9. Cohen, S., Allasia, V., Venard, P., Notter, S., Vernière, C., & Panabières, F. (2003). Intraspecific variation in Phytophthora citrophthora from citrus trees in eastern Corsica. European Journal of Plant Pathology, 109, 791-805.
10. Cooke, D. E. L., & Duncan, J. M. (1997). Phylogenetic analysis of Phytophthora species based on the ITS1 and ITS2 sequences of ribosomal DNA. Mycological Research, 101, 667-677.
11. Cooke, D. E. L., Drenth, A., Duncan, J. M., Wagels, G., & Brasier, C. M. (2000). A molecular phylogeny of Phytophthora and related oomycetes. Fungal Genetics and Biology, 30, 17-23.
12. Erwin, D. C., & Ribeiro, O. K. (1996). Phytophthora diseases worldwide. American Phytopathological Society Press, St. Paul, Minnesota.
13. Govers, F. (2001). Misclassification of pest as 'fungus' puts vital research on wrong track. Nature, 411, 633.
14. Graham, J. H. (1995). Root regeneration and tolerance of citrus rootstocks to root rot caused by Phytophthora nicotianae. Phytopathology, 85, 11-117.
15. Graham, J. H., & Menge, J. A. (1999). Root health: Fungal diseases. In L. W. Timmer & L. W. Duncan (Eds.), Citrus health management (pp. 126-135). St. Paul, Minnesota: American Phytopathological Society Press.
16. Graham, J. H., Timmer, L. W., Drouillard, D. L., & Peever, T. L. (1998). Characterization of Phytophthora spp., causing outbreaks of citrus brown rot in Florida. Phytopathology, 88, 724-729.
17. Graham, J. H., Bright, D. B., & McCoy, C. W. (2003). Phytophthora-Diaprepes weevil complex: Phytophthora spp. relationships with citrus rootstocks. Plant Disease, 87, 85-90.
18. Grimm, G. R., & Alexander, A. F. (1973). Citrus leaf pieces as traps for Phytophthora parasitica from soil slurries. Phytopathology, 63, 540-541.
19. Grosser, J. W., Graham, J. H., McCoy, C. W., Hoyte, A., Rubio, H. M., Bright, D. B., & Chandler, J. L. (2003). Development of "tetrazyg" rootstocks tolerant of the Diaprepes/Phytophthora complex under greenhouse conditions. Proceedings of the Florida State Horticultural Society, 116, 263-267.
20. Grote, D., Olmos, A., Kofoet, A., Tuset, J. J., Bertolini, E., & Cambra, M. (2002). Specific and sensitive detection of Phytophthora nicotianae by simple and nested-PCR. European Journal of Plant Pathology, 108, 197-207.
21. Ippolito, A., Schena, L., & Nigro, F. (2002). Detection of Phytophthora nicotianae and P. citrophthora in citrus roots and soils by nested PCR. European Journal of Plant Pathology, 108, 855-868.
22. Ippolito, A., Schena, L., Nigro, F., Ligorio, V. S., & Yaseen, T. (2004). Real-time detection of Phytophthora nicotianae and P. citrophthora in citrus roots and soil. European Journal of Plant Pathology, 110, 833-843.
23. Judelson, H. S., & Tooley, P. W. (2000). Enhanced polymerase chain reaction methods for detecting and quantifying Phytophthora infestans in plants. Phytopathology, 90, 1112-1119.
24. Lacourt, I., & Duncan, J. M. (1997). Specific detection of Phytophthora nicotianae using the polymerase chain reaction and primers based on the DNA sequences of its elicitin gene ParA1. European Journal of Plant Pathology, 103, 73-83.
25. Lee, S. B., & Taylor, J. W. (1992). Phylogeny of five fungus-like protoctistan Phytophthora species, inferred from the internal transcribed spacers of ribosomal DNA. Journal of Molecular Evolution, 9, 636-653.
26. Mchau, G. R. A., & Coffey, M. D. (1994). An integrated study of the morphological and isozyme patterns found within a worldwide collection of Phytophthora citrophthora and a redescription of the species. Mycological Research, 98, 1291-1299.
27. Miller, S. A. (1996). Detecting propagules of plant pathogenic fungi. In S. H. DeBoer (Ed.), Advances in botanical research, (Vol. 23, pp. 73-102). London: Academic Press.
28. Miller, S. A., Madden, L. V., & Schmitthenner, A. F. (1997). Distribution of Phytophthora spp. in field soils determined by immunoassays. Phytopathology, 87, 101-107.
29. Mohan, S. B. (1989). Cross-reactivity of antiserum raised against Phytophthora fragariae with other Phytophthora species and its evaluation as a genus-detecting antiserum. Plant Pathology, 38, 352-363.
30. Nechwatal, J., & Oßwald, W. (2001). Comparative studies on the fine root status of healthy and declining spruce and beech trees in the bavarian alps and occurrence of Phytophthora and Pythium species. Forest Pathology, 31, 257-273.
31. Nechwatal, J., Schlenzig, A., Jung, T., Cooke, D. E. L., Duncan, J. M., & Oßwald, W. (2001). A combination of baiting and PCR techniques for the detection of Phytophthora quercina and P. citricola in soil samples from oak stands. Forest Pathology, 31, 85-97.
32. Oudemans, P., & Coffey, M. D. (1991). A revised systematics of twelve papillate Phytophthora species based on isozyme analysis. Mycological Research, 95, 1025-1046.
33. Schaad, N. W., & Frederick, R. D. (2002). Real-time PCR and its application for rapid plant disease diagnosis. Canadian Journal of Plant Pathology, 24, 250-258.
34. Schubert, R., Bahnweg, G., Nechwatal, J., Jung, T., Cooke, D. E. L., Duncan, J. M., Müller-Starck, G., Langebartels, C., Sandermann, H., & Oßwald, W. (1999). Detection and quantification of Phytophthora species which are associated with root-rot diseases in European deciduous forests by species-specific polymerase chain reaction. European Journal of Forest Pathology, 29, 169-188.
35. Timmer, L. W., & Menge, J. A. (1988). Phytophthora-induced diseases. In J. O. Whiteside, S. M. Garnsey & L. W. Timmer (Eds.), Compendium of citrus diseases (pp. 22-24). St. Paul, Minnesota: American Phytopathological Society Press.
36. Timmer, L. W., Sandler, H. A., Graham, J. H., & Zitko, S. E. (1988). Sampling citrus orchards in Florida to estimate populations of Phytophthora parasitica. Phytopathology, 78, 940-944.
37. Timmer, L. W., Menge, J. A., Zitko, S. E., Pond, E., Miller, S. A., & Johnson, E. L. V. (1993). Comparison of ELISA techniques and standard isolation methods for Phytophthora detection in citrus orchards in Florida and California. Plant Disease, 77, 791-796.
38. Tooley, P. W., & Therrien, C. D. (1987). Cytophotometric determination of the nuclear DNA content of 23 Mexican and 18 non-Mexican isolates of Phytophthora infestans. Experimental Mycology, 11, 19-26.
39. Tooley, P. W., Bunyard, B. A., Carras, M. M., & Hatziloukas, E. (1997). Development of PCR primers from internal transcribed spacer region 2 for the detection of Phytophthora species infecting potatoes. Applied Environmental Microbiology, 63, 1467-1475.
40. Trout, C. L., Ristaino, J. B., Madritch, M., & Wangsomboondee, T. (1997). Rapid detection of Phytophthora infestans in late blight-infected potato and tomato using PCR. Plant Disease, 81, 1042-1048.
41. Tsao, P. H., & Guy, S. O. (1977). Inhibition of Mortierella and Pythium in a Phytophthora-isolation medium containing hymexazol. Phytopathology, 67, 796-801.
42. Tsao, P. H. (1990). Why many Phytophthora root rots and crown rots of tree and horticultural crops remain undetected. Bulletin Oepp/Eppo Bulletin, 20, 11-17.
43. Vandemark, G. J., & Barker, B. M. (2003). Quantifying Phytophthora medicaginis in susceptible and resistant alfalfa with a real-time fluorescent PCR assay. Journal of Phytopathology, 151, 577-583.
44. Vettraino, A. M., Natili, G., Anselmi, N., & Vannini, A. (2001). Recovery and pathogenicity of Phytophthora species associated with a resurgence of ink disease in Castanea sativa in Italy. Plant Pathology, 50, 90-96.
45. White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M. A. Innis, D. H. Gelfand J. J. Sninsky & T. J. White (Eds.), PCR protocols: A guide to methods and applications (pp. 315-321). New York: Academic Press.
46. Widmer, T. L., Graham, J. H., & Mitchell, D. J. (1998). Histological comparison of fibrous root infection of disease-tolerant and susceptible citrus hosts by Phytophthora nicotianae and P. palmivora. Phytopathology, 88, 389-395.
47. Zheng, F. C., & Ward, E. (1998). Variation within and between Phytophthora species from rubber and citrus trees in China, determined by polymerase chain reaction using RAPDs. Journal of Phytopathology, 146, 103-109.