Molecular characterization of specifically active recombinant fused enzymes consisting of CYP3A4, NADPH-cytochrome P450 oxidoreductase, and cytochrome b5

Biochemistry

Volumn 46, Issue 35, 2007, Pages 10213-10221

  Inui, Hideyuki ^a   Maeda, Atsuyo ^a   Ohkawa, Hideo ^a,b  

^a KOBE UNIVERSITY   (Japan)

^b FUKUYAMA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCATALYSTS; CHARACTERIZATION; HORMONES; HYDROXYLATION; MOLECULAR INTERACTIONS; SUBSTRATES; YEAST;

ELECTRON FLOW; OXIDOREDUCTASE; TESTOSTERONE;

ENZYME ACTIVITY;

CYTOCHROME B5; CYTOCHROME P450 3A4; HEME; NIFEDIPINE; RECOMBINANT ENZYME; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE FERRIHEMOPROTEIN REDUCTASE; TESTOSTERONE;

ARTICLE; BINDING AFFINITY; ELECTRON TRANSPORT; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME CONFORMATION; HYDROXYLATION; MICROSOME; MOLECULAR INTERACTION; NONHUMAN; OXIDATION; PRIORITY JOURNAL; REDUCTION; SACCHAROMYCES CEREVISIAE;

CYTOCHROME P-450 ENZYME SYSTEM; CYTOCHROMES B5; ELECTRON TRANSPORT; HUMANS; KINETICS; MICROSOMES; MIXED FUNCTION OXYGENASES; NADH, NADPH OXIDOREDUCTASES; NADPH-FERRIHEMOPROTEIN REDUCTASE; NIFEDIPINE; OXIDATION-REDUCTION; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TESTOSTERONE;

SACCHAROMYCES CEREVISIAE;

EID: 34548481755     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi700164q     Document Type: Article

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