An improved procedure for expression and purification of ribosomal protection protein Tet(O) for high-resolution structural studies

Protein Expression and Purification

Volumn 55, Issue 2, 2007, Pages 388-394

  Thakor, Nehal S ^a   Wilson, Kevin S ^a   Scott, Paul G ^a   Taylor, Diane E ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

Author keywords

Protein expression; Protein purification; Ribosomal protection proteins (RPPs); Tet(O); Tetracycline resistance

Indexed keywords

BACTERIAL PROTEIN; CARRIER PROTEIN; GUANOSINE TRIPHOSPHATE; PRIMER DNA; RECOMBINANT PROTEIN; TET O RESISTANCE PROTEIN, BACTERIA; UNCLASSIFIED DRUG;

ARTICLE; CHEMISTRY; CIRCULAR DICHROISM; GENETICS; HYDROLYSIS; ISOLATION AND PURIFICATION; LIGHT; LIQUID CHROMATOGRAPHY; METABOLISM; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN CONFORMATION; RADIATION SCATTERING; SOLUBILITY;

BACTERIAL PROTEINS; BASE SEQUENCE; CARRIER PROTEINS; CHROMATOGRAPHY, LIQUID; CIRCULAR DICHROISM; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GUANOSINE TRIPHOSPHATE; HYDROLYSIS; LIGHT; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SCATTERING, RADIATION; SOLUBILITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 34548429668     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2007.04.016     Document Type: Article

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