A colorimetric assay for the determination of acetyl xylan esterase or cephalosporin C acetyl esterase activities using 7-amino cephalosporanic acid, cephalosporin C, or acetylated xylan as substrate

Analytical Biochemistry

Volumn 369, Issue 2, 2007, Pages 210-217

  Martínez Martínez, Irene ^a   Montoro García, Silvia ^a   Lozada Ramírez, José Daniel ^a   Sánchez Ferrer, Álvaro ^a   García Carmona, Francisco ^a  

^a UNIVERSITY OF MURCIA   (Spain)

Author keywords

7 Aminocephalosporanic acid; Acetylated xylan; Cephalosporin C; Colorimetric assay; pH indicator

Indexed keywords

ANTIBIOTICS; BACTERIOLOGY; CLONING; COLOR; COLORIMETRY; ESCHERICHIA COLI; ESTERS; SUBSTRATES;

ACETYLATED XYLAN; CEPHALOSPORANIC ACID; CEPHALOSPORIN C; COEFFICIENT OF VARIANCE; COLORIMETRIC ASSAYS; DEACETYLASE ACTIVITY; ESCHERICHIA COLI ROSETTA (DE3); PH INDICATOR;

ENZYMES;

7 AMINOCEPHALOSPORANIC ACID; ACETYL ESTERASE; ACETYL XYLAN ESTERASE; BACTERIAL ENZYME; CEPHALOSPORIN C; ESTERASE; UNCLASSIFIED DRUG; XYLAN;

ACETYLATION; ARTICLE; BACILLUS PUMILUS; COLORIMETRY; CONTROLLED STUDY; DEACETYLATION; DRUG DETERMINATION; ENZYME ACTIVITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; PRIORITY JOURNAL; ROBOTICS;

BACILLUS PUMILUS; ESCHERICHIA COLI;

EID: 34548416230     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2007.06.030     Document Type: Article

References (23)

1. A. Sánchez-Ferrer, I. Martínez-Martínez, E. García-Pizarro, F. García-Carmona, Spanish patent application ES 200400346, 2004.
2. D.H. Choi, K.S. Han, I.S. Jeong, S.H. Lee, B.S. Lim, Y.D. Kim, WO patent 9955881, 1999 (Chem. Abstr. 131 (1999) 332993).
3. Coutinho P.M., and Henrissat B. Carbohydrate-active enzymes: An integrated database approach. In: Gilbert H.J., Davies G., Henrissat B., and Svensson B. (Eds). Recent Advances in Carbohydrate Bioengineering (1999), Royal Society of Chemistry, Cambridge, UK 3-12
4. Degrassi G., Kojic M., Ljubijankic G., and Venturi V. The acetyl xylan esterase of Bacillus pumilus belongs to a family of esterases with broad substrate specificity. Microbiology 146 (2000) 1585-1591
5. Biely P. Microbial xylanolytic system. Trends Biotechnol. 3 (1985) 286-290
6. Biely P., MacKenzie C.R., Puls J., and Schneider H. Cooperativity of esterases and xylanases in the enzymatic degradation of acetyl xylan. Biotechnology 4 (1986) 731-733
7. Whistler R.L., and Richards E.L. Hemicelluloses. In: Pigman W., and Horton D. (Eds). The Carbohydrates: Chemistry and Biochemistry (1970), Academic Press, New York 447-469
8. Lindberg B., Rossel K.G., and Svensson S. Position of the O-acetyl groups in birch xylan. Sven. Papperstidn. 76 (1973) 30-32
9. Schmidt M., and Bornscheuer U.T. High-throughput assays for lipases and esterases. Biomol. Eng. 22 (2005) 51-56
10. Baumann M., Stürmer R., and Bornscheuer U.T. A high-throughput-screening method for the identification of active and enantioselective hydrolases. Angew. Chem. Int. 40 (2001) 4201-4204
11. Patett F., and Fischer L. Spectrophotometric assay for quantitative determination of 7-aminocephalosporanic acid from direct hydrolysis of cephalosporin C. Anal. Biochem. 350 (2006) 304-306
12. Janes L.E., Lowendhal A.C., and Kazlauskas R.J. Quantitative screening of hydrolase libraries using pH indicators: Identifying active and enantioselective hydrolases. Chem. Eur. J. 4 (1998) 2324-2331
13. Varas F.M., Shah A., Aikens J., Nadkarni N.P., Rozzell J.D., and Demirjian D.C. Visualization of enzyme catalyzed reactions using pH indicators: Rapid screening of hydrolase libraries and estimation of enantioselectivity. Bioorg. Med. Chem. 7 (1999) 2183-2188
14. Banerjee A., Kaul P., Sharma R., and Banerjee U.C. A high-throughput amenable colorimetric assay for enantioselective screening of nitrilase-producing microorganisms using pH sensitive indicators. J. Biomol. Screen. 8 (2003) 559-565
15. Zhao H. A pH indicator based screening method for hydrolytic haloalkane dehalogenase. In: Arnold F.H., and Georgiou G. (Eds). Methods in Molecular Biology vol. 230 (2003), Humana, Totowa, NJ 213-221
16. Johnson K.G., Fontana J.D., and MacKenzie C.R. Measurement of acetylxylan esterase in Streptomyces. Methods Enzymol. 160 (1988) 551-560
17. Salazar O., and Sun L. Evaluating a screen and analysis of mutant libraries. In: Arnold F.H., and Georgiou G. (Eds). Methods in Molecular Biology vol. 230 (2003), Humana, Totowa, NJ 85-97
18. Martínez-Martínez I., Navarro-Fernández J., Lozada-Ramirez D., García-Carmona F., and Sánchez-Ferrer A. Maximization of the production of His-tagged glycine oxidase and its M261 mutant proteins. Biotechnol. Prog. 22 (2006) 647-652
19. Vincent F., Charnock S.J., Verschueren K.H.G., Turkenburg J.P., Scott D.J., Offen W.A., Roberts S., Pell G., Gilbert H.J., Davies G.J., and Brannigan J.A. Multifunctional xylooligosaccharide/cephalosporin C deacetylase revealed by the hexameric structure of the Bacillus subtilis enzyme at 1.9 Ǻ resolution. J. Mol. Biol. 330 (2003) 593-606
20. Morin R.B., Jackson B.G., Mueller R.A., Lavagnino E.R., Scanlon W.B., and Andrews S.L. Chemistry of cephalosporin antibiotics: XV. Transformations of penicillin sulfoxide: A synthesis of cephalosporin compounds. J. Am. Chem. Soc. 91 (1969) 1401-1407
21. Kukolja S. Chemistry of cephalosporin antibiotics: XI. Preparation and properties of desacetylcephaloglycin and its lactone. J. Med. Chem. 11 (1968) 1067-1069
22. Beynon R.J., and Easterby J.S. Buffer Solutions (1996), Oxford University Press, Oxford, UK
23. Lorenz W.W., and Wiegel J. Isolation, analysis, and expression of two genes from Thermoanaerobacterium sp. strain JW/SL YS485: A β-xylosidase and a novel acetyl xylan esterase with cephalosporin C deacetylase activity. J. Bacteriol. 179 (1997) 5436-5441