Maximization of production of his-tagged glycine oxidase and its M261 mutant proteins

Biotechnology Progress

Volumn 22, Issue 3, 2006, Pages 647-652

  Martínez Martínez, Irene ^a   Navarro Fernández, José ^a   Lozada Ramírez, José D ^a   García Carmona, Francisco ^a   Sánchez Ferrer, Álvaro ^a  

^a UNIVERSITY OF MURCIA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CELL CULTURE; ENZYME KINETICS; ESCHERICHIA COLI; FERMENTATION; GENES; PROTEINS;

ENZYMATIC ACTIVITY; GLYCINE OXIDASE (GOX); PHARMACOLOGICAL INDUSTRY; PLASMID;

ENZYMES;

GLYCINE OXIDASE; HISTIDINE; OXIDOREDUCTASE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BACILLUS SUBTILIS; BIOSYNTHESIS; CHEMISTRY; COMPARATIVE STUDY; ENZYME ACTIVATION; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETICS; KINETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MUTATION; PHYSIOLOGY; SENSITIVITY AND SPECIFICITY;

AMINO ACID OXIDOREDUCTASES; AMINO ACID SEQUENCE; BACILLUS SUBTILIS; CLONING, MOLECULAR; ENZYME ACTIVATION; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HISTIDINE; KINETICS; MOLECULAR SEQUENCE DATA; MUTATION; RECOMBINANT PROTEINS; SENSITIVITY AND SPECIFICITY;

BACILLUS SUBTILIS; ESCHERICHIA COLI;

EID: 33745012843     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp050397g     Document Type: Article

References (23)

1. Baca, A. M.; Hol, W. G. J. Overcoming codon bias: a method for high-level overexpression of Plasmodium and other AT-rich parasite genes in Escherichia coli. Int. J. Parasitol. 2000, 30, 113-118.
2. Bradford, M. M. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72, 248-254.
3. Brodelius, P.; Nilsson, K.; Mosbach, K. Production of α-keto acids. I. Immobilized cells of Trigonopsis variabilis containing D-amino acid oxidase. Appl. Biochem. Biotechnol. 1981, 6, 293-308.
4. Butó, S.; Pollegioni, L.; D'Angiuro, L.; Pilone, M. S. Evaluation of D-amino acid oxidase from Rhodotorula gracilis for the production of α-keto acids: a reactor system. Biotechnol. Bioeng. 1994, 44, 1288-1294.
5. Conlon, H. D.; Baqal, J. Baker, K.; Shen, Y. Q.; Wong, B. L.; Nolles, R.; Rausch, C. W. Two step immobilized enzyme conversion of cephalosporin C to 7-aminocephalosporanic acid. Biotechnol. Bioeng. 1995, 46, 510-513.
6. Gemeiner, P.; Stefuca, V.; Welwardova, A.; Michalkova, E.; Welward, L.; Kurillova, L.; Danielsson, B. Direct determination of the cephalosporin transforming activity of immobilized cells with use of an enzyme thermistor. I. Verification of the mathematical model. Enzyme Microb. Technol. 1993, 15, 50-56.
7. Gomi, K.; Horiuchi, T. Purification and characterization of a new enzyme, N-alkylglycine oxidase from Cladosporium sp. G-10. Biochim. Biophys. Acta 1999, 1429, 439-45.
8. Job, V.; Molla, G.; Pilone, M. S.; Pollegioni, L. Overexpression of a recombinant wild-type and His-tagged Bacillus subtilis glycine oxidase in Escherichia coli. Eur. J. Biochem. 2002a, 269, 1456-1463.
9. Job, V.; Marcone, G. L.; Pilone, M. S.; Pollegioni, L. Glycine oxidase from Bacillus subtilis. J. Biol. Chem. 2002b, 277, 6987-6993.
10. Kane, J. F. Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli. Curr. Opin. Biotechnol. 1995, 6, 494-500.
11. Kunst, F.; Ogasawara, N.; Moszer, I.; Albertini, A. M.; Alloni, G.; Azevedo, V.; Bertero, M. G. et al. The complete genome sequence of the Gram-positive bacterium Bacillus subtilis. Nature 1997, 390, 249-256.
12. Kurland, C.; Galland, J. Errors of heterologous protein expression. Curr. Opin. Biotechnol. 1996, 7, 489-493.
13. Lin, L. L.; Chien, H. R.; Wang, W. C.; Hwang, T. S.; Fu, H. M.; Hsu, W. H. Expression of Trigonopsis variabilis D-amino acid oxidase gene in Escherichia coli and characterization of its inactive mutants. Enzyme Microb. Technol. 2000, 27, 482-491.
14. Médigue, C.; Rouxel, T.; Vigier, P.; Hénaut, A.; Danchin, A. Evidence for horizontal gene transfer in Escherichia coli speciation. J. Mol. Biol. 1991, 222, 851-856.
15. Mörtl, M.; Diederichs, K.; Weite, W.; Molla, G.; Motteran, L.; Andriolo, G.; Pilon, M. S.; Pollegioni, L. Structure-function correlation in glycine oxidase from Bacillus subtilis. J. Biol. Chem. 2004, 279, 29718-29727.
16. Nakajima, N.; Conrad, D.; Sumi, H.; Suzuki, K.; Esaki, N.; Wandrey, C.; Soda, K. Continuous conversion to optically pure L-methionine from D-enantiomer contaminated preparations by an immobilized enzyme membrane reactor. J. Ferment. Technol. 1990, 70, 322-325.
17. Nishiya, Y.; Imanaka, T. Purification and characterization of a novel glycine oxidase from Bacillus subtilis. FEBS Lett. 1998, 438, 263-266.
18. Pilone, M. S.; Buto, S.; Pollegioni, L. A process for bioconversion of cephalosporin C by Rhodotorula gracilis D-amino acid oxidase. Biotechnol. Lett. 1995, 17, 199-204.
19. Sambrook, J.; Fritsch, E. P.; Maniatis, T. Molecular Cloning: A Laboratory Manual, 2nd ed.; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY, 1989.
20. Sánchez-Ferrer, A.; Martínez-Martínez, I.; García-Pizarro, E.; García-Carmona, F. Patent application ES200400346, 2004.
21. Settembre, E. C.; Dorrestein, P. C.; Park, J. H.; Augustine, A. M.; Begley, T. P.; Ealick, S. E. Structural and mechanistic studies on ThiO, a glycine oxidase essential for thiamine biosynthesis in Bacillus subtilis. Biochemistry 2003, 42, 2971-2981.
22. Szwajcer-Dey, E.; Flygare, S.; Mosbach, K. Stabilitation of D-amino acid oxidase from yeast Trigonopsis variabilis used for production of glutaryl-7-aminocephalosporanic acid from cephalosporanic C. Appl. Biochem. Biotechnol. 1991, 27, 239-250.
23. Trost, E. M.; Fischer, L. Minimization of byproduct formation during D-amino acid oxidase catalyzed racemate resolution of D/L-amino acids. J. Mol. Catal. B 2002, 19-20, 189-195.