Detection, quantification and identification of fungal extracellular laccases using polyclonal antibody and mass spectrometry

Enzyme and Microbial Technology

Volumn 41, Issue 6-7, 2007, Pages 694-701

  Kellner, Harald ^a,c   Jehmlich, Nico ^a   Benndorf, Dirk ^a   Hoffmann, Ralf ^d   Rühl, Martin ^b   Hoegger, Patrik J ^b   Majcherczyk, Andrzej ^b   Kües, Ursula ^b   von Bergen, Martin ^a   Buscot, François ^a,c  

^a HELMHOLTZ CENTRE FOR ENVIRONMENTAL RESEARCH UFZ   (Germany)

^b UNIVERSITY OF GÖTTINGEN   (Germany)

^c UNIVERSITY OF LEIPZIG   (Germany)

^d Center for Biotechnology and Biomedicine   (Germany)

Author keywords

Basidiomycetes; Copper binding region; Native PAGE; SDS PAGE; Tandem mass spectrometry; Western blot

Indexed keywords

BASIDIOMYCETES; COPPER BINDING REGIONS; FUNGAL EXTRACELLULAR LACCASES; TANDEM MASS SPECTROMETRY;

ANTIBODIES; COPPER; DATA ACQUISITION; DATABASE SYSTEMS; FUNGI; GENES; MASS SPECTROMETRY; PEPTIDES;

ENZYME KINETICS;

COPPER; FUNGAL ENZYME; LACCASE; LACCASE 1; PEPTIDE; POLYCLONAL ANTIBODY; UNCLASSIFIED DRUG;

ANTIBODY SPECIFICITY; ARTICLE; ASCOMYCETES; BASIDIOMYCETES; BINDING SITE; COMPUTER MODEL; COPRINOPSIS CINEREA; DATA BASE; ENZYME ANALYSIS; ENZYME CONFORMATION; ENZYME DENATURATION; FUNGUS; HYPHOLOMA FASCICULARE; IMMUNOASSAY; LENTINUS EDODES; LIQUID CHROMATOGRAPHY; MYCENA; NONHUMAN; NUCLEOTIDE SEQUENCE; PIRIFORMOSPORA INDICA; PLEUROTUS; PLEUROTUS CORNUCOPIAE; PLEUROTUS OSTREATUS; PYCNOPORUS CINNABARINUS; QUANTITATIVE ANALYSIS; TANDEM MASS SPECTROMETRY; TRAMETES VERSICOLOR; VERPA CONICA;

BASIDIOMYCOTA; COPRINOPSIS CINEREA; HYPHOLOMA FASCICULARE; LENTINULA EDODES; MYCENA; PIRIFORMOSPORA INDICA; PLEUROTUS CORNUCOPIAE; PLEUROTUS OSTREATUS; PYCNOPORUS CINNABARINUS; TRAMETES VERSICOLOR; VERPA CONICA;

EID: 34548407753     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2007.06.002     Document Type: Article

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