Enhancement of cell proliferation and differentiation by combination of ascorbate and dexamethasone in thermo-reversible hydrogel constructs embedded with rabbit chondrocytes

Biotechnology Letters

Volumn 29, Issue 10, 2007, Pages 1453-1457

  Na, Kun ^a   Choi, Seong Jun ^b   Kim, Sungwon ^c   Sun, Bo Kyung ^d   Woo, Dae Gyun ^d   Chung, Hyung Min ^b   Park, Keun Hong ^b  

^a The Catholic University of Korea   (South Korea)

^b CHA University   (South Korea)

^c Korea Institute of Science and Technology   (South Korea)

^d Chabiotech Co Ltd   (South Korea)

Author keywords

Ascorbate; Cartilage; Chondrocytes; Dexamethasone; Hydrogel

Indexed keywords

CARTILAGE; COLLAGEN; GENES; HYDROGELS;

DEXAMETHASONE; HYALINE CARTILAGE; RABBIT CHONDROCYTES;

CELL PROLIFERATION;

ANTIINFLAMMATORY AGENT; ANTIOXIDANT; ASCORBIC ACID; COLLAGEN; DEXAMETHASONE;

ANIMAL; ARTICLE; CARTILAGE CELL; CELL DIFFERENTIATION; CELL PROLIFERATION; CHEMISTRY; CYTOLOGY; DRUG EFFECT; HYDROGEL; IMMUNOHISTOCHEMISTRY; METABOLISM; MOUSE; NUDE MOUSE; RABBIT;

ANIMALS; ANTI-INFLAMMATORY AGENTS; ANTIOXIDANTS; ASCORBIC ACID; CELL DIFFERENTIATION; CELL PROLIFERATION; CHONDROCYTES; COLLAGEN; DEXAMETHASONE; HYDROGEL; IMMUNOHISTOCHEMISTRY; MICE; MICE, NUDE; RABBITS;

ORYCTOLAGUS CUNICULUS;

EID: 34548131694     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-007-9420-8     Document Type: Article

References (12)

1. Barbucci R, Lamponi S, Borzacchiello A, Ambrosio L, Fini M, Torricelli P, Giardino R (2002) Hyaluronic acid hydrogel in the treatment of osteoarthritis. Biomaterials 23:4503-4513
2. Calcagno M, Coyena H, Arrambide E, Amuti de Urse C (1970) Action of cortisone and cortisol upon biosynthesis of chondroitin sulfate in femur in vitro cultures of chick embryo. Exp Cell Res 63:131-137
3. Derfoul A. Perkins GL, Hall DJ, Tuan RS (2006) Glucocortiocoids promote chondrogenic differentiation of adult human mesenchymal stem cells by enhancing expression of cartilage extracellular matrix genes. Stem Cells 24:1487-1495
4. Giannoni P, Crovace A, Malpeli M, Maggi E, Arbico R, Cancedda R, Dozin B (2005) Species variability in the differentiation potential of in vitro-expanded articular chondrocytes restricts predictive studies on cartilage repair using animal models. Tissue Eng 11:237-248
5. Grigoriadis AE, Aubin JE, Heersche JN (1989) Effects of dexamethasone and vitamin D3 on cartilage differentiation in a clonal chondrogenic cell population. Endocrinology 125:2103-2110
6. Hoemann CD, Sun J, Legare A, McKee MD, Buschmann MD (2005) Tissue engineering of cartilage using an injectable and adhesive chitosan-based cell-delivery vehicle. Osteoarthr Cartil 13:318-329
7. Malda J, Blitterswijk CA, Geffen M, Martens DE, Tramper J, Riesle J (2004) Low oxygen tension stimulates the redifferentiation of dedifferentiated adult human nasal chondrocytes. Osteoarthr Cartil 12:306-313
8. Na K, Park JH, Kim S, Sun BK, Chung HM, Park KH (2007) Blended construct consisting of thermo-reversible hydrogels and heparinized nanoparticles for increasing proliferation activity of rabbit chondrocyte in vivo test. Biotechnol Lett Accepted for publication
9. Park KH, Na K, Kim SW, Jung SY, Park KH, Chung HM (2005) Phenotype of hepatocyte spheroids behavior within thermo-sensitive poly(NiPAAm-co-PEG-g- GRGDS) hydrogel as a cell delivery vehicle. Biotechnol Lett 27:1081-1086
10. Stewart MC, Saunders KM, Burton-Wurster N, Macleod JN (2000) Phenotypic stability of articular chondrocytes in vitro: the effects of culture models, bone morphogenetic protein 2, and serum supplementation. J Bone Miner Res 15:166-174
11. Syftestad GT, Weitzhandler M, Caplan AI (1985) Isolation and characterization of osteogenic cells derived from first bone of the embryonic tibia. Dev Biol 110:275-283
12. Wang SY, Roguska MA, Gudas LJ (1989) Defective post-translational modification of collagen IV in a mutant F9 teratocarcinoma cell line is associated with delayed differentiation and growth arrest in response to retinoic acid. J Biol Chem 15:15556-15564