Conservation of NADPH utilization by chorismate synthase and its implications for the evolution of the shikimate pathway

Molecular Microbiology

Volumn 65, Issue 5, 2007, Pages 1249-1257

  Ehammer, Heidemarie ^a   Rauch, Gernot ^a   Prem, Anna ^a   Kappes, Barbara ^b   Macheroux, Peter ^a  

^a GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^b UNIVERSITY HOSPITAL HEIDELBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CHORISMIC ACID; FLAVINE MONONUCLEOTIDE; OXIDOREDUCTASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; SHIKIMIC ACID; SYNTHETASE;

ARTICLE; CONTROLLED STUDY; IN VIVO STUDY; NONHUMAN; PRIORITY JOURNAL; PROTOZOON; SACCHAROMYCES CEREVISIAE;

BACTERIAL PROTEINS; EVOLUTION, MOLECULAR; FLAVIN MONONUCLEOTIDE; FUNGAL PROTEINS; ISOENZYMES; NADP; PHENOTYPE; PHOSPHORUS-OXYGEN LYASES; SACCHAROMYCES CEREVISIAE; SHIKIMIC ACID;

BACTERIA (MICROORGANISMS); EUGLENA GRACILIS; FUNGI; MYXOZOA; PROTOZOA; SACCHAROMYCES CEREVISIAE;

EID: 34547917580     PISSN: 0950382X     EISSN: 13652958     Source Type: Journal    
DOI: 10.1111/j.1365-2958.2007.05861.x     Document Type: Article

References (31)

1. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. Struhl, K. (1994) Current Protocols in Molecular Biology. New York : Greene Publishing Associates and Wiley Interscience.
2. Bornemann, S., Lowe, D.J. Thorneley, R.N.F. (1996) The transient kinetics of Escherichia coli chorismate synthase: substrate consumption, product formation, phosphate dissociation and characterisation of a flavin intermediate. Biochemistry 35 : 9907 9916.
3. Campbell, S.A., Richards, T.A., Mui, E.J., Samuel, U., Coggins, J.R., McLeod, R. Roberts, C.W. (2004) A complete shikimate pathway in Toxoplasma gondii: an ancient eukaryotic innovation. Int J Parasitol 34 : 5 13.
4. Duncan, K., Edwards, R.M. Coggins, J.R. (1987) The pentafunctional arom enzyme of Saccharomyces cerevisiae is a mosaic of monofunctional domains. Biochem J 246 : 375 386.
5. Fitzpatrick, T.B., Ricken, S., Lanzer, M., Amrhein, N., Macheroux, P. Kappes, B. (2001) Subcellular localization and characterization of chorismate synthase in the apicomplexan Plasmodium falciparum. Mol Microbiol 40 : 65 75.
6. Gietz, R.D. Woods, R.A. (1998) Transformation of yeast by the lithium acetate/single-stranded carrier DNA/PEG method. Methods Microbiol 26 : 53 66.
7. Gill, S.C. von Hippel, P.H. (1989) Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem 182 : 319 326.
8. Hasan, N. Nester, E.W. (1978a) Purification and characterization of NADPH-dependent flavin reductase. An enzyme required for the activation of chorismate synthase in Bacillus subtilis. J Biol Chem 253 : 4987 4992.
9. Hasan, N. Nester, E.W. (1978b) Purification and properties of chorismate synthase from Bacillus subtilis. J Biol Chem 253 : 4993 4998.
10. Henstrand, J.M., Amrhein, N. Schmid, J. (1995) Cloning and characterization of a heterologously expressed bifunctional chorismate synthase/flavin reductase from Neurospora crassa. J Biol Chem 270 : 20447 20452.
11. Henstrand, J.M., Schaller, A., Braun, M., Amrhein, N. Schmid, J. (1996) Saccharomyces cerevisiae chorismate synthase has a flavin reductase activity. Mol Microbiol 22 : 859 866.
12. Hoffmann, C.S. Winston, F. (1987) A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli. Gene 57 : 267 272.
13. Horvath, A. Riezmann, H. (1994) Rapid protein extraction from yeast. Yeast 10 : 1305 1310.
14. Ito, J., Cox, E.C. Yanofsky, C. (1969) Anthranilate synthetase, an enzyme specified by the tryptophan operon of Escherichia coli: purification and characterization of component I. J Bacteriol 97 : 725 733.
15. Jones, D.G.L., Reusser, U. Braus, G.H. (1991) Molecular cloning, characterization and analysis of the regulation of the ARO2 gene, encoding chorismate synthase, of Saccharomyces cerevisiae. Mol Microbiol 5 : 2143 2152.
16. Kitzing, K., Macheroux, P. Amrhein, N. (2001) Spectroscopic and kinetic characterization of the bifunctional chorismate synthase from Neurospora crassa. Evidence for a common binding site for 5-enolpyruvylshikimate 3-phosphate and NADPH. J Biol Chem 276 : 42658 42666.
17. Kitzing, K., Auweter, S., Amrhein, N. Macheroux, P. (2004) Mechanism of chorismate synthase. Role of the two invariant histidine residues in the active site. J Biol Chem 279 : 9451 9461.
18. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 : 680 685.
19. Lowry, O.H., Rosebrough, N.J., Farr, A.L. Randall, R.J. (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193 : 265 275.
20. Macheroux, P., Schmid, J., Amrhein, N. Schaller, A. (1999) A unique reaction in a common pathway: mechanism and function of chorismate synthase in the shikimate pathway. Planta 207 : 325 334.
21. McConkey, G.A., Pinney, J.W., Westhead, D.R., Plueckhahn, K., Fitzpatrick, T.B., Macheroux, P. Kappes, B. (2004) Annotating the Plasmodium genome and the enigma of the shikimate pathway. Trends Parasitiol 20 : 60 65.
22. Maclean, J. Ali, S. (2003) The structure of chorismate synthase reveals a novel flavin-binding site fundamental to a unique chemical reaction. Structure 11 : 1499 1511.
23. Morokutti, A., Lyskowski, A., Sollner, S., Pointner, E., Fitzpatrick, T.B., Kratky, C., et al. (2005) Structure and function of YcnD from Bacillus subtilis, a flavin-containing oxidoreductase. Biochemistry 44 : 13724 13733.
24. Osborne, A., Thorneley, R.N.F., Abell, C. Bornemann, S. (2000) Studies with substrate and cofactor analogues provide evidence for a radical mechanism in the chorismate synthase reaction. J Biol Chem 275 : 35825 35830.
25. Quevillon-Cheruel, S., Leulliot, N., Meyer, P., Graille, M., Bremang, M., Blondeau, K., et al. (2004) Crystal structure of the bifunctional chorismate synthase from Saccharomyces cerevisiae. J Biol Chem 279 : 619 625.
26. Reinbothe, C., Ortel, B., Parthier, B. Reinbothe, S. (1994) Cytosolic and plastid forms of 5-enolpyruvylshikimate 3-phosphate synthase in Euglena gracilis are differently expressed during light-induced chloroplast development. Mol Gen Genet 245 : 616 622.
27. Richards, T., Dacks, J.B., Campbell, S.A., Blanchard, J.L., Foster, P.G., McLeod, R. Roberts, C.W. (2006) Evolutionary origins of the eukaryotic shikimate pathway: gene fusions, horizontal gene transfer, and endosymbiotic replacements. Eukaryotic Cell 5 : 1517 1531.
28. Schaller, A., Windhofer, V. Amrhein, N. (1990) Purification of chorismate synthase from a cell culture of the higher plant Corydalis sempervirens Pers. Arch Biochem Biophys 282 : 437 442.
29. Schaller, A., van Afferden, M., Windhofer, V., Bülow, S., Abel, G., Schmid, J. Amrhein, N. (1991) Purification and characterization of chorismate synthase from Euglena gracilis. Plant Physiol 97 : 1271 1279.
30. Welch, G.R., Cole, K.W. Gaertner, F.H. (1974) Chorismate synthase of Neurospora crassa: a flavoprotein. Arch Biochem Biophys 165 : 505 518.
31. Winzeler, E.A., Shoemaker, D.D., Astromoff, A., Liang, H., Anderson, K., Andre, B., et al. (1999) Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science 285 : 901 906.