Saccharomyces cerevisiae chorismate synthase has a flavin reductase activity

Molecular Microbiology

Volumn 22, Issue 5, 1996, Pages 859-866

  Henstrand, John M ^a,b   Schaller, Andreas ^b   Braun, Martin ^b   Amrhein, Nikolaus ^b   Schmid, Jürg ^b  

^a PLANT GENE EXPRESSION CENTER   (United States)

^b ETH ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

CHORISMATE SYNTHASE; CHORISMIC ACID; FLAVIN REDUCTASE; QUERCETIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME KINETICS; NONHUMAN; PHYLOGENY; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE;

BACTERIA (MICROORGANISMS); FUNGI; NEUROSPORA CRASSA; SACCHAROMYCES CEREVISIAE;

EID: 0029825116     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1996.01534.x     Document Type: Article

References (27)

1. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K. (1992) Current Protocols in Molecular Biology. New York: Greene Publishing Associates and Wiley-Interscience.
2. Benner, S.A., Baddcoe, I., Cohen, M.A., and Gerloff, D.L. (1994) Bona fide prediction of aspects of protein conformation. J Mol Biol 235: 926-958.
3. Bornemann, S., Balasubramanian, S., Coggins, J.R., Abell, C., Lowe, D.J., and Thorneley, R.N.F. (1995) Escherichia coli chorismate synthase: a deuterium kinetic-isotope effect under single-turnover and steady-state conditions shows that a flavin intermediate forms before the C-(6proR)-H bond is cleaved. Biochem J 305: 707-710.
4. Braun, M., Henstrand, J.M., Görlach, J., Amrhein, N., and Schmid, J. (1996) Enzymatic properties of chorismate synthase isozymes of tomato (Lycopersicon esculentum Mill.). Planta 200: 64-70.
5. Charles, I.G., Lamb, H.K., Pickard., D., Bougan, G., and Hawkins, A.R. (1990) Characterization and nucleotide sequences of the AroC genes encoding chorismate synthase from Salmonella typhi and Escherichia coli. J Gen Microbiol 136: 353-358.
6. Görlach, J., Beck, A., Henstrand, J.M., Handa, A.K., Herrmann, K.M., Schmid, J., and Amrhein, N. (1993) Differential expression of tomato (Lycopersicon esculentum) genes encoding shikimate pathway isoenzymes. II. Chorismate synthase. Plant Mol Biol 23: 707-716.
7. Hasan, N., and Nester, E.W. (1978a) Purification and properties of chorismate synthase from Bacillus subtilis. J Biol Chem 253: 4993-4998.
8. Hasan, N., and Nester, E.W. (1978b) Purification and characterization of NADPH-dependent flavin reductase. J Biol Chem 253: 4987-4992.
9. Hawkins, A.R., and Smith, M. (1991) Domain structure and interaction within the pentafunctional arom polypeptide. Eur J Biochem 196: 717-724.
10. Henstrand, J.M., Schmid, J., and Amrhein, N. (1995a) Only the mature form of plastidic chorismate synthase is enzymatically active. Plant Physiol 108: 1127-1132.
11. Henstrand, J.M., Amrhein, N., and Schmid, J. (1995b) Cloning and characterization of a heterologously expressed bifunctional chorismate synthase/flavin reductase from Neurospora crassa. J Biol Chem 270: 20447-20452.
12. Jones, D.G.L., Reusser, U., and Braus, G.H. (1991) Molecular cloning, characterization and analysis of the regulation of the Aro2 gene, encoding chorismate synthase, of Saccharomyces cerevisiae. Mol Microbiol 5: 2143-2152.
13. Macheroux, P., Petersen, J., Bornemann, S., Lowe, D.J., and Thorneley, R.N.F. (1996) Binding of the oxidized, reduced, and radical flavin species to chorismate synthase. An investigation by spectrophotometry, fluorimetry, and electron paramagnetic resonance and electron nuclear double resonance spectroscopy. Biochemistry 35: 1643-1652.
14. Minet, M., Dufour, M.-M., and Lacroute, F. (1992) Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNAs. Plant J 2: 417-421.
15. Pittard, J., and Wallace, B.J. (1966) Distribution and function of genes concerned with aromatic biosynthesis in Escherichia coli. J Bacteriol 91: 1494-1508.
16. Ramjee, M.N., Coggins, J.R., Hawkes, T.R., Lowe, D.J., and Thorneley, R.N.F. (1991) Spectrophotometric detection of a modified flavin mononucleotide (FMN) intermediate formed during the catalytic cycle of chorismate synthase. J Am Chem Soc 113: 8566-8567.
17. Ramjee, M.N., Balasubramanian, S., Abell, C., Coggins, J.R., Davies, G.M., Hawkes, T.R., Lowe, D.J., and Thorneley, R.N.F. (1992) Reaction of (6R)-6-F-EPSP with recombinant Escherichia coli chorismate synthase generates a stable flavin mononucleotide semiquinone radical. J Am Chem Soc 114: 3151-3153.
18. Rothstein, R.J. (1983) One-step gene disruption in yeast. Meth Enzymol 101: 202-211.
19. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. New York: Cold Spring Harbor Laboratory Press.
20. Sanger, F., Nicklen, S., and Coulson, A.R. (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 71: 5463-5467.
21. Schaller, A., Windhofer, V., and Amrhein, N. (1990) Purification of chorismate synthase from a cell culture of the higher plant Corydalis sempervirens Pers. Arch Biochem Biophys 282: 437-442.
22. Schaller, A., van Afferden, M., Windhofer, V., Bülow, S., Abel, G., Schmid, J., and Amrhein, N. (1991a) Purification and characterization of chorismate synthase from Euglena gracilis. Plant Physiol 97: 1271-1279.
23. Schaller, A., Schmid, J., Leibinger, U., and Amrhein, N. (1991b) Molecular cloning and analysis of a cDNA coding for chorismate synthase from the higher plant Corydalis sempervirens Pers. J Biol Chem 266: 21434-21438.
24. Schmidt, J., Bubunenko, M., and Subramanian, A.R. (1993) A novel operon organization involving the genes for chorismate synthase (aromatic biosynthesis pathway) and ribosomal GTPase center proteins (L11, L1, L10, L12: rplKAJL) in cyanobacterium Synechocystis PCC 6803. J Biol Chem 268: 27447-27457.
25. Welch, G.R., Cole, K.W., and Gaertner, F.H. (1974) Chorismate synthase of Neurospora crassa: a flavoprotein. Arch Biochem Biophys 165: 505-518.
26. White, P.J., Millar, G., and Coggins, J.R. (1988) The overexpression, purification and complete amino acid sequence of chorismate synthase from Escherichia coli K12 and its comparison with the enzyme from Neurospora crassa. Biochem J 251: 313-322.
27. Wierenga, R.K., Terpstra, P., and Hol, W.G.J. (1986) Prediction of occurrence of the ADP-binding βαβ-fold in proteins, using an amino acid sequence fingerprint. J Mol Biol 187: 101-107.