Spectroscopic and kinetic characterization of the bifunctional chorismate synthase from Neurospora crassa: Evidence for a common binding site for 5-enolpyruvylshikimate 3-phosphate and NADPH

Journal of Biological Chemistry

Volumn 276, Issue 46, 2001, Pages 42658-42666

  Kitzing, Karina ^a   Macheroux, Peter ^a   Amrhein, Nikolaus ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BIOSYNTHESIS; CATALYST ACTIVITY; ENZYME KINETICS; ENZYMES; ESCHERICHIA COLI; SPECTROSCOPIC ANALYSIS;

HOMOGENEITY;

BIOCHEMISTRY;

5 ENOLPYRUVYLSHIKIMATE 3 PHOSPHATE; AROMATIC AMINO ACID; CHORISMATE SYNTHASE; CHORISMIC ACID; DIHYDROLIPOAMIDE DEHYDROGENASE; FLAVINE MONONUCLEOTIDE; FUNGAL ENZYME; OXIDOREDUCTASE; OXYGEN; PYRUVIC ACID DERIVATIVE; QUERCETIN; RECOMBINANT ENZYME; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE DEHYDROGENASE; REDUCING AGENT; SHIKIMIC ACID DERIVATIVE; UNCLASSIFIED DRUG; 5 ENOLPYRUVOYLSHIKIMATE 3 PHOSPHATE; 5-ENOLPYRUVOYLSHIKIMATE-3-PHOSPHATE; COMPLEMENTARY DNA; DRUG DERIVATIVE; LYASE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; SHIKIMIC ACID;

AMINO ACID SYNTHESIS; ARTICLE; BINDING SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME BINDING; ENZYME KINETICS; ENZYME MECHANISM; ENZYME METABOLISM; ENZYME PURIFICATION; ESCHERICHIA COLI; NEUROSPORA CRASSA; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN STRUCTURE; REDUCTION; SPECTROSCOPY; CHEMICAL MODEL; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; KINETICS; METABOLISM; MOLECULAR CLONING; PLASMID; PROTEIN BINDING; PROTEIN TERTIARY STRUCTURE; SITE DIRECTED MUTAGENESIS; SPECTROFLUOROMETRY; SPECTROPHOTOMETRY; TIME; ULTRAVIOLET RADIATION;

ESCHERICHIA COLI; NEUROSPORA; NEUROSPORA CRASSA;

BINDING SITES; CLONING, MOLECULAR; DNA, COMPLEMENTARY; ESCHERICHIA COLI; FLAVIN MONONUCLEOTIDE; KINETICS; MODELS, CHEMICAL; MUTAGENESIS, SITE-DIRECTED; NADP; NEUROSPORA CRASSA; OXIDOREDUCTASES; OXYGEN; PHOSPHORUS-OXYGEN LYASES; PLASMIDS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; SHIKIMIC ACID; SPECTROMETRY, FLUORESCENCE; SPECTROPHOTOMETRY; SUBSTRATE SPECIFICITY; TIME FACTORS; ULTRAVIOLET RAYS;

EID: 0035900664     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M107249200     Document Type: Article

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