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Volumn 21, Issue 5-6, 2007, Pages 368-378

Fast and sensitive quantitative detection of HIV DNA in whole blood leucocytes by SYBR green I real-time PCR assay

Author keywords

HIV DNA quantification; Human endogenous retroviruses; Primer binding site; SYBR Green I real time PCR; Whole blood leucocytes

Indexed keywords

ANTIRETROVIRUS AGENT; FLUORESCENT DYE; PRIMER DNA; SYBR GREEN 1; UNCLASSIFIED DRUG; VIRUS DNA;

EID: 34547898048     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mcp.2007.05.005     Document Type: Article
Times cited : (35)

References (45)
  • 1
    • 0034040118 scopus 로고    scopus 로고
    • Reservoirs for HIV-1: mechanisms for viral persistence in the presence of antiviral immune responses and antiretroviral therapy
    • Pierson T., McArthur J., and Siliciano R.F. Reservoirs for HIV-1: mechanisms for viral persistence in the presence of antiviral immune responses and antiretroviral therapy. Annu Rev Immunol 18 (2000) 665-708
    • (2000) Annu Rev Immunol , vol.18 , pp. 665-708
    • Pierson, T.1    McArthur, J.2    Siliciano, R.F.3
  • 2
    • 0036176509 scopus 로고    scopus 로고
    • The challenge of viral reservoirs in HIV-1 infection
    • Blankson J.N., Persaud D., and Siliciano R.F. The challenge of viral reservoirs in HIV-1 infection. Annu Rev Med 53 (2002) 557-593
    • (2002) Annu Rev Med , vol.53 , pp. 557-593
    • Blankson, J.N.1    Persaud, D.2    Siliciano, R.F.3
  • 3
    • 27644524761 scopus 로고    scopus 로고
    • HIV-infected individuals receiving effective antiviral therapy for extended periods of time continually replenish their viral reservoir
    • Chun T.W., Nickle D.C., Justement J.S., Large D., Semerjian A., Curlin M.E., et al. HIV-infected individuals receiving effective antiviral therapy for extended periods of time continually replenish their viral reservoir. J Clin Invest 115 (2005) 3250-3255
    • (2005) J Clin Invest , vol.115 , pp. 3250-3255
    • Chun, T.W.1    Nickle, D.C.2    Justement, J.S.3    Large, D.4    Semerjian, A.5    Curlin, M.E.6
  • 4
    • 33745245733 scopus 로고    scopus 로고
    • Residual human immunodeficiency virus type 1 viremia in some patients on antiretroviral therapy is dominated by a small number of invariant clones rarely found in circulating CD4+ T cells
    • Bailey J.R., Sedaghat A., Kieffer T., Brennan T., Lee P.K., Wind-Rotolo M., et al. Residual human immunodeficiency virus type 1 viremia in some patients on antiretroviral therapy is dominated by a small number of invariant clones rarely found in circulating CD4+ T cells. J Virol 80 (2006) 6441-6457
    • (2006) J Virol , vol.80 , pp. 6441-6457
    • Bailey, J.R.1    Sedaghat, A.2    Kieffer, T.3    Brennan, T.4    Lee, P.K.5    Wind-Rotolo, M.6
  • 5
    • 33747122666 scopus 로고    scopus 로고
    • Treatment for adult HIV infection: 2006 recommendations of the International AIDS Society-USA panel
    • Hammer S.M., Saag M.S., Schechter M., Montaner J.S., Schooley R.T., Jacobsen D.M., et al. Treatment for adult HIV infection: 2006 recommendations of the International AIDS Society-USA panel. JAMA 296 (2006) 827-843
    • (2006) JAMA , vol.296 , pp. 827-843
    • Hammer, S.M.1    Saag, M.S.2    Schechter, M.3    Montaner, J.S.4    Schooley, R.T.5    Jacobsen, D.M.6
  • 6
    • 1642506123 scopus 로고    scopus 로고
    • Impact of 5 years of maximally successful highly active antiretroviral therapy on CD4 cell count and HIV-1 DNA level
    • Viard J.P., Burgard M., Hubert J.B., Aaron L., Rabian C., Pertuiset N., et al. Impact of 5 years of maximally successful highly active antiretroviral therapy on CD4 cell count and HIV-1 DNA level. AIDS 18 (2004) 45-49
    • (2004) AIDS , vol.18 , pp. 45-49
    • Viard, J.P.1    Burgard, M.2    Hubert, J.B.3    Aaron, L.4    Rabian, C.5    Pertuiset, N.6
  • 8
    • 33144485618 scopus 로고    scopus 로고
    • Agence Nationale de Recherche sur le Sida PRIMO Study Group. CD4 cell count and HIV DNA level are independent predictors of disease progression after primary HIV type 1 infection in untreated patients
    • Goujard C., Bonarek M., Meyer L., Bonnet F., Chaix M.L., Deveau C., et al. Agence Nationale de Recherche sur le Sida PRIMO Study Group. CD4 cell count and HIV DNA level are independent predictors of disease progression after primary HIV type 1 infection in untreated patients. Clin Infect Dis 42 (2006) 709-715
    • (2006) Clin Infect Dis , vol.42 , pp. 709-715
    • Goujard, C.1    Bonarek, M.2    Meyer, L.3    Bonnet, F.4    Chaix, M.L.5    Deveau, C.6
  • 9
    • 0033980093 scopus 로고    scopus 로고
    • PCR-Based assay to quantify human immunodeficiency virus type 1 DNA in peripheral blood mononuclear cells
    • Christopherson C., Kidane Y., Conway B., Krowka J., Sheppard H., and Kwok S. PCR-Based assay to quantify human immunodeficiency virus type 1 DNA in peripheral blood mononuclear cells. J Clin Microbiol 38 (2000) 630-634
    • (2000) J Clin Microbiol , vol.38 , pp. 630-634
    • Christopherson, C.1    Kidane, Y.2    Conway, B.3    Krowka, J.4    Sheppard, H.5    Kwok, S.6
  • 10
    • 33748052966 scopus 로고    scopus 로고
    • Comparative analysis of T-cell turnover and homeostatic parameters in HIV-infected patients with discordant immune-virological responses to HAART
    • Marchetti G., Gori A., Casabianca A., Magnani M., Pranzetti F., Clerici M., et al. Comparative analysis of T-cell turnover and homeostatic parameters in HIV-infected patients with discordant immune-virological responses to HAART. AIDS 20 (2006) 1727-1736
    • (2006) AIDS , vol.20 , pp. 1727-1736
    • Marchetti, G.1    Gori, A.2    Casabianca, A.3    Magnani, M.4    Pranzetti, F.5    Clerici, M.6
  • 11
    • 4744346009 scopus 로고    scopus 로고
    • Swiss HIV Cohort Study. Proviral HIV-DNA predicts viral rebound and viral setpoint after structured treatment interruptions
    • Yerly S., Gunthard H.F., Fagard C., Joos B., Perneger T.V., Hirschel B., et al. Swiss HIV Cohort Study. Proviral HIV-DNA predicts viral rebound and viral setpoint after structured treatment interruptions. AIDS. 18 (2004) 1951-1953
    • (2004) AIDS. , vol.18 , pp. 1951-1953
    • Yerly, S.1    Gunthard, H.F.2    Fagard, C.3    Joos, B.4    Perneger, T.V.5    Hirschel, B.6
  • 14
    • 21244496808 scopus 로고    scopus 로고
    • Antiretroviral compounds: mechanisms underlying failure of HAART to eradicate HIV-1
    • Shehu-Xhilaga M., Tachedjian G., Crowe S.M., and Kedzierska K. Antiretroviral compounds: mechanisms underlying failure of HAART to eradicate HIV-1. Curr Med Chem 12 (2005) 1705-1719
    • (2005) Curr Med Chem , vol.12 , pp. 1705-1719
    • Shehu-Xhilaga, M.1    Tachedjian, G.2    Crowe, S.M.3    Kedzierska, K.4
  • 15
    • 33644540503 scopus 로고    scopus 로고
    • Marcello A. Latency: the hidden HIV-1 challenge. Retrovirology 2006. doi:10.1186/1742-4690-3-7.
  • 17
    • 0012140441 scopus 로고    scopus 로고
    • Failure of routine HIV-1 tests in a case involving transmission with preseroconversion blood components during the infectious window period
    • Ling A.E., Robbins K.E., Brown T.M., Dunmire V., Thoe S.Y., Wong S.Y., et al. Failure of routine HIV-1 tests in a case involving transmission with preseroconversion blood components during the infectious window period. JAMA 284 (2000) 210-214
    • (2000) JAMA , vol.284 , pp. 210-214
    • Ling, A.E.1    Robbins, K.E.2    Brown, T.M.3    Dunmire, V.4    Thoe, S.Y.5    Wong, S.Y.6
  • 18
    • 0030768855 scopus 로고    scopus 로고
    • Dynamics of HIV-1 mRNA expression in patients with long-term nonprogressive HIV-1 infection
    • Comar M., Simonelli C., Zanussi S., Paoli P., Vaccher E., Tirelli U., et al. Dynamics of HIV-1 mRNA expression in patients with long-term nonprogressive HIV-1 infection. J Clin Invest 100 (1997) 893-903
    • (1997) J Clin Invest , vol.100 , pp. 893-903
    • Comar, M.1    Simonelli, C.2    Zanussi, S.3    Paoli, P.4    Vaccher, E.5    Tirelli, U.6
  • 19
    • 0033027958 scopus 로고    scopus 로고
    • Level of human immunodeficiency virus DNA in peripheral blood mononuclear cells correlates with efficacy of antiretroviral therapy
    • McDermott J.L., Giri A.A., Martini I., Bono M., Giacobini M., Campelli A., et al. Level of human immunodeficiency virus DNA in peripheral blood mononuclear cells correlates with efficacy of antiretroviral therapy. J Clin Microbiol 37 (1999) 2361-2365
    • (1999) J Clin Microbiol , vol.37 , pp. 2361-2365
    • McDermott, J.L.1    Giri, A.A.2    Martini, I.3    Bono, M.4    Giacobini, M.5    Campelli, A.6
  • 20
    • 0034485793 scopus 로고    scopus 로고
    • Quantification of HIV-1 proviral DNA in patients with undetectable plasma viremia over long-term highly active antiretroviral therapy
    • Debiaggi M., Zara F., Pistorio A., Bruno R., Sacchi P., Patrono S.F., et al. Quantification of HIV-1 proviral DNA in patients with undetectable plasma viremia over long-term highly active antiretroviral therapy. Int J Infect Dis 4 (2000) 187-193
    • (2000) Int J Infect Dis , vol.4 , pp. 187-193
    • Debiaggi, M.1    Zara, F.2    Pistorio, A.3    Bruno, R.4    Sacchi, P.5    Patrono, S.F.6
  • 21
    • 0037217732 scopus 로고    scopus 로고
    • Prognostic value of baseline human immunodeficiency virus type 1 DNA measurement for disease progression in patients receiving nucleoside therapy
    • Tierney C., Lathey J.L., Christopherson C., Bettendorf D.M., D'Aquila R.T., Hammer S.M., et al. Prognostic value of baseline human immunodeficiency virus type 1 DNA measurement for disease progression in patients receiving nucleoside therapy. J Infect Dis 187 (2003) 144-148
    • (2003) J Infect Dis , vol.187 , pp. 144-148
    • Tierney, C.1    Lathey, J.L.2    Christopherson, C.3    Bettendorf, D.M.4    D'Aquila, R.T.5    Hammer, S.M.6
  • 22
    • 0035059676 scopus 로고    scopus 로고
    • Quantification of human immunodeficiency virus type 1 proviral load by a TaqMan real-time PCR assay
    • Désiré N., Dehée A., Schneider V., Jacomet C., Goujon C., Girard P.M., et al. Quantification of human immunodeficiency virus type 1 proviral load by a TaqMan real-time PCR assay. J Clin Microbiol 39 (2001) 1303-1310
    • (2001) J Clin Microbiol , vol.39 , pp. 1303-1310
    • Désiré, N.1    Dehée, A.2    Schneider, V.3    Jacomet, C.4    Goujon, C.5    Girard, P.M.6
  • 23
    • 0036784560 scopus 로고    scopus 로고
    • The Multicenter Hemophilia Cohort Study Group. Quantitation of human immunodeficiency virus type 1 DNA forms with the second template switch in peripheral blood cells predicts disease progression independently of plasma RNA load
    • Kostrikis L.G., Touloumi G., Karanicolas R., Pantazis N., Anastassopoulou C., Karafoulidou A., et al. The Multicenter Hemophilia Cohort Study Group. Quantitation of human immunodeficiency virus type 1 DNA forms with the second template switch in peripheral blood cells predicts disease progression independently of plasma RNA load. J Virol 76 (2002) 10099-10108
    • (2002) J Virol , vol.76 , pp. 10099-10108
    • Kostrikis, L.G.1    Touloumi, G.2    Karanicolas, R.3    Pantazis, N.4    Anastassopoulou, C.5    Karafoulidou, A.6
  • 24
    • 0141742220 scopus 로고    scopus 로고
    • A multiplex real-time PCR for quantification of HIV-1 DNA and the human albumin gene in CD4+ cells
    • Eriksson L.E., Leitner T., Wahren B., Bostrom A.C., and Falk K.I. A multiplex real-time PCR for quantification of HIV-1 DNA and the human albumin gene in CD4+ cells. APMIS 111 (2003) 625-633
    • (2003) APMIS , vol.111 , pp. 625-633
    • Eriksson, L.E.1    Leitner, T.2    Wahren, B.3    Bostrom, A.C.4    Falk, K.I.5
  • 25
    • 1442286420 scopus 로고    scopus 로고
    • Quantitative detection of human immunodeficiency virus type 1 (HIV-1) proviral DNA in peripheral blood mononuclear cells by SYBR green real-time PCR technique
    • Gibellini D., Vitone F., Schiamone P., Ponti C., La Placa M., and Re M.C. Quantitative detection of human immunodeficiency virus type 1 (HIV-1) proviral DNA in peripheral blood mononuclear cells by SYBR green real-time PCR technique. J Clin Virol 29 (2004) 282-289
    • (2004) J Clin Virol , vol.29 , pp. 282-289
    • Gibellini, D.1    Vitone, F.2    Schiamone, P.3    Ponti, C.4    La Placa, M.5    Re, M.C.6
  • 26
    • 25444515465 scopus 로고    scopus 로고
    • Kabamba-Mukadi B, Henrivaux P, Ruelle J, Del ferriere N, Bodeus M, Goubau P, Human immunodeficiency virus type 1 (HIV-1) proviral DNA load in purified CD4+ cells by LightCycler real-time PCR BMC. Infect Dis 2005. doi:10.1186/1471-2334-5-15.
  • 27
    • 0035161903 scopus 로고    scopus 로고
    • Simple, sensitive, and specific detection of human immunodeficiency virus type 1 subtype B DNA in dried blood samples for diagnosis in infants in the field
    • Beck I.A., Drennan K.D., Melvin A.J., Mohan K.M., Herz A.M., Alarcon J., et al. Simple, sensitive, and specific detection of human immunodeficiency virus type 1 subtype B DNA in dried blood samples for diagnosis in infants in the field. J Clin Microbiol 39 (2001) 29-33
    • (2001) J Clin Microbiol , vol.39 , pp. 29-33
    • Beck, I.A.1    Drennan, K.D.2    Melvin, A.J.3    Mohan, K.M.4    Herz, A.M.5    Alarcon, J.6
  • 28
    • 16844379582 scopus 로고    scopus 로고
    • Detection of human immunodeficiency virus type 1 DNA in dried blood spots by a duplex real-time PCR assay
    • Luo W., Yang H., Rathbun K., Pau C.P., and Ou C.Y. Detection of human immunodeficiency virus type 1 DNA in dried blood spots by a duplex real-time PCR assay. J Clin Microbiol 43 (2005) 1851-1857
    • (2005) J Clin Microbiol , vol.43 , pp. 1851-1857
    • Luo, W.1    Yang, H.2    Rathbun, K.3    Pau, C.P.4    Ou, C.Y.5
  • 29
    • 0031776457 scopus 로고    scopus 로고
    • A comprehensive panel of near-full-length clones and reference sequences for non-subtype B isolates of human immunodeficiency virus type 1
    • Gao F., Robertson D.L., Carruthers C.D., Morrison S.G., Jian B., Chen Y., et al. A comprehensive panel of near-full-length clones and reference sequences for non-subtype B isolates of human immunodeficiency virus type 1. J Virol 72 (1998) 5680-5698
    • (1998) J Virol , vol.72 , pp. 5680-5698
    • Gao, F.1    Robertson, D.L.2    Carruthers, C.D.3    Morrison, S.G.4    Jian, B.5    Chen, Y.6
  • 30
    • 4644241496 scopus 로고    scopus 로고
    • Development of a real-time PCR assay using SYBR Green I for provirus load quantification in a murine model of AIDS
    • Casabianca A., Orlandi C., Fraternale A., and Magnani M. Development of a real-time PCR assay using SYBR Green I for provirus load quantification in a murine model of AIDS. J Clin Microbiol 42 (2004) 4361-4364
    • (2004) J Clin Microbiol , vol.42 , pp. 4361-4364
    • Casabianca, A.1    Orlandi, C.2    Fraternale, A.3    Magnani, M.4
  • 31
    • 34547890105 scopus 로고    scopus 로고
    • Gregory TR. 2007. Animal Genome Size Database 〈http://www.genomesize.com〉.
  • 32
    • 0025951290 scopus 로고
    • Deletions in the tRNA(Lys) primer-binding site of human immunodeficiency virus type 1 identify essential regions for reverse transcription
    • Rhim H., Park J., and Morrow C.D. Deletions in the tRNA(Lys) primer-binding site of human immunodeficiency virus type 1 identify essential regions for reverse transcription. J Virol 65 (1991) 4555-4564
    • (1991) J Virol , vol.65 , pp. 4555-4564
    • Rhim, H.1    Park, J.2    Morrow, C.D.3
  • 33
    • 0035944932 scopus 로고    scopus 로고
    • Destabilization of tRNA3(Lys) from the primer-binding site of HIV-1 genome by anti-A loop polyamide nucleotide analog
    • Kaushik N., Talele T.T., Monel R., Palumbo P., and Pandey V.N. Destabilization of tRNA3(Lys) from the primer-binding site of HIV-1 genome by anti-A loop polyamide nucleotide analog. Nucleic Acids Res 29 (2001) 5099-5106
    • (2001) Nucleic Acids Res , vol.29 , pp. 5099-5106
    • Kaushik, N.1    Talele, T.T.2    Monel, R.3    Palumbo, P.4    Pandey, V.N.5
  • 34
    • 33644774584 scopus 로고    scopus 로고
    • Inhibition of human immunodeficiency virus type 1 replication with artificial transcription factors targeting the highly conserved primer-binding site
    • Eberhardy S.R., Goncalves J., Coelho S., Segal D.J., Berkhout B., and Barbas III C.F. Inhibition of human immunodeficiency virus type 1 replication with artificial transcription factors targeting the highly conserved primer-binding site. J Virol 80 (2006) 2873-2883
    • (2006) J Virol , vol.80 , pp. 2873-2883
    • Eberhardy, S.R.1    Goncalves, J.2    Coelho, S.3    Segal, D.J.4    Berkhout, B.5    Barbas III, C.F.6
  • 35
    • 0037841763 scopus 로고    scopus 로고
    • Transcription start regions in the human genome are favored targets for MLV integration
    • Wu X., Li Y., Crise B., and Burgess S.M. Transcription start regions in the human genome are favored targets for MLV integration. Science 300 (2003) 1749-1751
    • (2003) Science , vol.300 , pp. 1749-1751
    • Wu, X.1    Li, Y.2    Crise, B.3    Burgess, S.M.4
  • 37
    • 0036216132 scopus 로고    scopus 로고
    • Evaluation of a home-made SYBR green I reaction mixture for real-time PCR quantification of gene expression
    • Karsai A., Muller S., Platz S., and Hauser M.T. Evaluation of a home-made SYBR green I reaction mixture for real-time PCR quantification of gene expression. Biotechniques 32 (2002) 790-796
    • (2002) Biotechniques , vol.32 , pp. 790-796
    • Karsai, A.1    Muller, S.2    Platz, S.3    Hauser, M.T.4
  • 38
    • 0034917315 scopus 로고    scopus 로고
    • Griffiths DJ. Endogenous retroviruses in the human genome sequence. Genome Biol 2001. doi:10.1186/gb-2001-2-6-reviews1017.
  • 39
    • 2442720089 scopus 로고    scopus 로고
    • Expression of the human endogenous retrovirus HERV-W family in various human tissues and cancer cells
    • Yi J.M., Kim H.M., and Kim H.S. Expression of the human endogenous retrovirus HERV-W family in various human tissues and cancer cells. J Gen Virol 85 (2004) 1203-1210
    • (2004) J Gen Virol , vol.85 , pp. 1203-1210
    • Yi, J.M.1    Kim, H.M.2    Kim, H.S.3
  • 40
    • 33750341578 scopus 로고    scopus 로고
    • The evolutionary dynamics of human endogenous retroviral families
    • Bannert N., and Kurth R. The evolutionary dynamics of human endogenous retroviral families. Annu Rev Genomics Hum Genet 7 (2006) 149-173
    • (2006) Annu Rev Genomics Hum Genet , vol.7 , pp. 149-173
    • Bannert, N.1    Kurth, R.2
  • 41
    • 34547878896 scopus 로고    scopus 로고
    • User Bulletin No. 2, ABI Prism 7700 Sequence Detection System, December 11, 1997. Foster City: Applied Biosystems.
  • 43
    • 0026546362 scopus 로고
    • Mutation in the primer binding site of the type 1 human immunodeficiency virus genome affects virus production and infectivity
    • Nagashunmugam T., Velpandi A., Goldsmith C.S., Zaki S.R., Kalyanaraman V.S., and Srinivasan A. Mutation in the primer binding site of the type 1 human immunodeficiency virus genome affects virus production and infectivity. Proc Natl Acad Sci. USA 89 (1992) 4114-4118
    • (1992) Proc Natl Acad Sci. USA , vol.89 , pp. 4114-4118
    • Nagashunmugam, T.1    Velpandi, A.2    Goldsmith, C.S.3    Zaki, S.R.4    Kalyanaraman, V.S.5    Srinivasan, A.6
  • 44
    • 0033609374 scopus 로고    scopus 로고
    • Persistence of HIV-1 transcription in peripheral-blood mononuclear cells in patients receiving potent antiretroviral therapy
    • Furtado M.R., Callaway D.S., Phair J.P., Kunstman K.J., Stanton J.L., Macken C.A., et al. Persistence of HIV-1 transcription in peripheral-blood mononuclear cells in patients receiving potent antiretroviral therapy. N Engl J Med 340 (1999) 1614-1622
    • (1999) N Engl J Med , vol.340 , pp. 1614-1622
    • Furtado, M.R.1    Callaway, D.S.2    Phair, J.P.3    Kunstman, K.J.4    Stanton, J.L.5    Macken, C.A.6
  • 45
    • 0036162182 scopus 로고    scopus 로고
    • Quantification of human immunodeficiency virus type 1 proviral DNA by using TaqMan technology
    • Zhao Y., Yu M., Miller J.W., Chen M., Bremer E.G., Kabat W., et al. Quantification of human immunodeficiency virus type 1 proviral DNA by using TaqMan technology. J Clin Microbiol 40 (2002) 675-678
    • (2002) J Clin Microbiol , vol.40 , pp. 675-678
    • Zhao, Y.1    Yu, M.2    Miller, J.W.3    Chen, M.4    Bremer, E.G.5    Kabat, W.6


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