Immunological evaluation of Escherichia coli expressed E2 protein of Western equine encephalitis virus

Virus Research

Volumn 128, Issue 1-2, 2007, Pages 26-33

  Das, Dipankar ^a   Nagata, Les P ^b   Suresh, Mavanur R ^a,b  

^a UNIVERSITY OF ALBERTA   (Canada)

^b DEFENCE RESEARCH AND DEVELOPMENT CANADA   (Canada)

Author keywords

Cell mediated immune response; E2 glycoprotein; Humoral response; Subunit vaccine; WEEV

Indexed keywords

GLYCOPROTEIN E2; NEUTRALIZING ANTIBODY; RECOMBINANT PROTEIN; SUBUNIT VACCINE;

AFRICAN TRYPANOSOMIASIS; ANIMAL EXPERIMENT; ANTIBODY RESPONSE; ARTICLE; BACTERIAL STRAIN; BLOOD SAMPLING; CELL INCLUSION; CELLULAR IMMUNITY; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FEMALE; HUMORAL IMMUNITY; IMMUNE RESPONSE; IMMUNOBLOTTING; IMMUNOGENICITY; MOLECULAR CLONING; MOUSE; NONHUMAN; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; SEROLOGY; VACCINATION; WESTERN EQUINE ENCEPHALOMYELITIS ALPHAVIRUS;

ANIMALS; ANTIBODIES, VIRAL; ENCEPHALITIS VIRUS, WESTERN EQUINE; ENCEPHALOMYELITIS, WESTERN EQUINE; ESCHERICHIA COLI; FEMALE; IMMUNIZATION; INCLUSION BODIES; LYMPHOCYTE ACTIVATION; MICE; MICE, INBRED BALB C; RECOMBINANT PROTEINS; T-LYMPHOCYTES; VIRAL ENVELOPE PROTEINS; VIRAL VACCINES;

ESCHERICHIA COLI; MUS; WESTERN EQUINE ENCEPHALOMYELITIS VIRUS;

EID: 34547884725     PISSN: 01681702     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.virusres.2007.03.030     Document Type: Article

References (23)

1. Boere W.A., Benaissa-Trouw B.J., Harmsen M., Kraaijeveld C.A., and Snippe H. Neutralizing and non-neutralizing monoclonal antibodies to the E2 glycoprotein of Semliki Forest virus can protect mice from lethal encephalitis. J. Gen. Virol. 64 Pt 6 (1983) 1405-1408
2. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-254
3. Das D., Gares S.L., Nagata L.P., and Suresh M.R. Evaluation of a Western Equine Encephalitis recombinant E1 protein for protective immunity and diagnostics. Antivir. Res. 64 2 (2004) 85-92
4. Ertl H.C., and Xiang Z. Novel vaccine approaches. J. Immunol. 156 10 (1996) 3579-3582
5. Frolov I., Hoffman T.A., Pragai B.M., Dryga S.A., Huang H.V., Schlesinger S., and Rice C.M. Alphavirus-based expression vectors: strategies and applications. Proc. Natl. Acad. Sci. U.S.A. 93 21 (1996) 11371-11377
6. Griffin D.E., and Mendoza Q.P. Identification of the inflammatory cells present in the central nervous system of normal and mast cell-deficient mice during Sindbis virus encephalitis. Cell Immunol. 97 2 (1986) 454-459
7. Griffin D., Levine B., Tyor W., Ubol S., and Despres P. The role of antibody in recovery from alphavirus encephalitis. Immunol. Rev. 159 (1997) 155-161
8. Hahn C.S., Lustig S., Strauss E.G., and Strauss J.H. Western equine encephalitis virus is a recombinant virus. Proc. Natl. Acad. Sci. U.S.A. 85 16 (1988) 5997-6001
9. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 5259 (1970) 680-685
10. Long M.C., Jager S., Mah D.C.W., Jebailey L., Mah M.A., Masri S.A., and Nagata L.P. Construction and characterization of a novel recombinant single-chain variable fragment antibody against Western equine encephalitis virus. Hybridoma 19 1 (2000) 1-13
11. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65 1-2 (1983) 55-63
12. Nagata L.P., Hu W.G., Masri S.A., Rayner G.A., Schmaltz F.L., Das D., Wu J., Long M.C., Chan C., Proll D., Jager S., Jebailey L., Suresh M.R., and Wong J.P. Efficacy of DNA vaccination against western equine encephalitis virus infection. Vaccine 23 17-18 (2005) 2280-2283
13. Netolitzky D.J., Schmaltz F.L., Parker M.D., Rayner G.A., Fisher G.R., Trent D.W., Bader D.E., and Nagata L.P. Complete genomic RNA sequence of western equine encephalitis virus and expression of the structural genes. J. Gen. Virol. 81 1 (2000) 151-159
14. Pedersen Jr. C.E., Robinson D.M., and Cole Jr. F.E. Isolation of the vaccine strain of Venezuelan equine encephalomyelitis virus from mosquitoes in Louisiana. Am. J. Epidemiol. 95 5 (1972) 490-496
15. Sambrook J., Fritsch E.F., and Maniatis T. Molecular Cloning: A Laboratory Manual (1989), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
16. Schlesinger S., and Schlesinger M.J. Togaviridae: the viruses and their replication. In: Fields B.N., Knipe D.M., and Howley P.M. (Eds). Fields Virology. third ed. (1996), Lippincott-Rave, Philadelphia 825-841
17. Schoepp R.J., Smith J.F., and Parker M.D. Recombinant chimeric western and eastern equine encephalitis viruses as potential vaccine candidates. Virology 302 2 (2002) 299-309
18. Strauss J.H., and Strauss E.G. The alphaviruses: gene expression, replication, and evolution. Microbiol. Rev. 58 3 (1994) 491-562
19. Strauss E.G., Stec D.S., Schmaljohn A.L., and Strauss J.H. Identification of antigenically important domains in the glycoproteins of Sindbis virus by analysis of antibody escape variants. J. Virol. 65 9 (1991) 4654-4664
20. Towbin H., Staehelin T., and Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. U.S.A. 76 9 (1979) 4350-4354
21. Turell M.J., O'Guinn M.L., and Parker M.D. Limited potential for mosquito transmission of genetically engineered, live-attenuated western equine encephalitis virus vaccine candidates. Am. J. Trop. Med. Hyg. 68 2 (2003) 218-221
22. Valenzuela P., Medina A., Rutter W.J., Ammerer G., and Hall B.D. Synthesis and assembly of hepatitis B virus surface antigen particles in yeast. Nature 298 5872 (1982) 347-350
23. Yurkova M.S., Patel A.H., and Fedorov A.N. Characterisation of bacterially expressed structural protein E2 of hepatitis C virus. Protein Exp. Purif. 37 1 (2004) 119-125