Characterisation of bacterially expressed structural protein E2 of hepatitis C virus

Protein Expression and Purification

Volumn 37, Issue 1, 2004, Pages 119-125

  Yurkova, Maria S ^a,b   Patel, Arvind H ^c   Fedorov, Alexey N ^a,b  

^a Research Center of Molecular Diagnostics and Therapy   (Russian Federation)

^b Centre of Biomolecular Technologies   (Russian Federation)

^c INSTITUTE OF VIROLOGY   (United Kingdom)

Author keywords

CD81; E2; HCV; Hepatitis C virus; Structural glycoprotein; Virus receptor interaction

Indexed keywords

CD81 ANTIGEN; GLYCOPROTEIN E2, HEPATITIS C VIRUS; HYBRID PROTEIN; LEUKOCYTE ANTIGEN; MONOCLONAL ANTIBODY; VIRUS ENVELOPE PROTEIN;

ANIMAL; ARTICLE; CHEMISTRY; ESCHERICHIA COLI; GENETICS; HEPATITIS C VIRUS; HUMAN; METABOLISM; PROTEIN CONFORMATION; PROTEIN TERTIARY STRUCTURE;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIGENS, CD; ESCHERICHIA COLI; HEPACIVIRUS; HUMANS; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; VIRAL ENVELOPE PROTEINS;

ESCHERICHIA COLI; HEPATITIS C VIRUS;

EID: 3543063551     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.05.016     Document Type: Article

References (18)

1. Houghton M. Hepatitis C viruses. Fields B.N., Knipe D.M., Howley P.M. Fields Virology. third ed.:1996;1035-1058 Lippincott-Raven, Philadelphia
2. Op De Beek A., Cocquerel L., Dubuisson J. Biogenesis of hepatitis C virus envelope glycoproteins. J. Gen. Virol. 82:2001;2589-2594
3. Choukhi A., Ung S., Wychowski C., Dubuisson J. Involvement of endoplasmic reticulum chaperones in the folding of hepatitis C virus glycoproteins. J. Virol. 72:1998;3851-3858
4. Deleersnyder V., Pillez A., Wychowski C., Blight K., Xu J., Hahn Y.S., Rice C.M., Dubuisson J. Formation of native hepatitis C virus glycoprotein complexes. J. Virol. 71:1997;697-704
5. Dubuisson J., Hsu H.H., Cheung R.C., Greenberg H.B., Russell D.G., Rice C.M. Formation and intracellular-localization of hepatitis-C virus envelope glycoprotein complexes expressed by recombinant vaccinia and sindbis viruses. J. Virol. 68:1994;6147-6160
6. Pileri P., Uematsu Y., Campagnoli S., Galli G., Falugi F., Petracca R., Weiner A.J., Houghton M., Rosa D., Grandi G., Abrignani S. Binding of hepatitis C virus to CD81. Science. 282:1998;938-941
7. Flint M., Maidens C., Loomis-Price L.D., Shotton C., Dubuisson J., Monk P., Higginbottom A., Levy S., McKeating J.A. Characterization of hepatitis C virus E2 glycoprotein interaction with a putative cellular receptor, CD81. J. Virol. 73:1999;6235-6244
8. Owsianka A., Clayton R.F., Loomis-Price L.D., McKeating J.A., Patel A.H. Functional analysis of hepatitis C virus E2 glycoproteins and virus-like particles reveals structural dissimilarities between different forms of E2. J. Gen. Virol. 82:2001;1877-1883
9. Heile J.M., Fong Y.-L., Rosa D., Berger K., Saletti G., Campagnoli S., Bensi G., Capo S., Coates S., Crawford K., Dong C., Wininger M., Baker G., Cousens L., Chien D., Ng P., Archangel P., Grandi G., Houghton M., Abrignani S. Evaluation of hepatitis C virus glycoprotein E2 for vaccine design: an endoplasmic reticulum-retained recombinant protein is superior to secreted recombinant protein and DNA-based vaccine candidates. J. Virol. 74:2000;6885-6892
10. Yanagi M., St.Claire M., Shapiro M., Emerson S.U., Purcell R.H., Bukh J. Transcripts of a chimeric cDNA clone of hepatitis C virus genotype 1b are infective in vivo. Virology. 244:1998;161-172
11. Clayton R.F., Owsianka A., Aitken J., Graham S., Bhella D., Patel A.H. Analysis of antigenicity and topology of E2 glycoprotein present on recombinant hepatitis C virus-like particles. J. Virol. 76:2002;7672-7682
12. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA. 76:1979;4350-4354
13. Yi M., Kaneko S., Yu D.Y., Murakami S. Hepatitis C virus envelope proteins bind lactoferrin. J. Virol. 71:1997;5997-6002
14. Patel A.H., Wood J., Penin F., Dubuisson J., McKeating J.A. Construction and characterization of chimeric hepatitis C virus E2 glycoproteins: analysis of regions critical for glycoprotein aggregation and CD81 binding. J. Gen. Virol. 81:2000;2873-2883
15. Rosa D., Campagnoli S., Moretto C., Guenzi E., Cousens L., Chin M., Dong C., Weiner A.J., Lau J.Y.N., Choo Q.L., Chien D., Pileri P., Houghton M., Abrignani S.C. A quantitative test to estimate neutralizing antibodies to the hepatitis C virus: cytofluorimetric assessment of envelope glycoprotein 2 binding to target cells. Proc. Natl. Acad. Sci. USA. 93:1996;1759-1763
16. Cocquerel L., Meunier J.C., Pillez A., Wychowski C., Dubuisson J. A retention signal necessary and sufficient for endoplasmic reticulum localization maps to the transmembrane domain of hepatitis C virus glycoprotein E2. J. Virol. 72:1998;2183-2191
17. Valenzuela P., Medina A., Rutter W.J., Ammerer G., Hall B.D. Synthesis and assembly of hepatitis B virus surface antigen particles in yeast. Nature. 298:1982;347-350
18. Stephenne J. Development and production aspects of a recombinant yeast-derived hepatitis B vaccine. Vaccine. 8(Suppl.):1990;69-73