Purification of the Prep1 interactome identifies novel pathways regulated by Prep1

Proteomics

Volumn 7, Issue 15, 2007, Pages 2617-2623

  Díaz, Víctor M ^a,d   Bachi, Angela ^b   Blasi, Francesco ^a,c  

^a VITA SALUTE SAN RAFFAELE UNIVERSITY   (Italy)

^b SAN RAFFAELE HOSPITAL   (Italy)

^c FIRC INSTITUTE OF MOLECULAR ONCOLOGY   (Italy)

^d INST MUNIC D INVESTIGACIO MEDICA   (Spain)

Author keywords

Homeoprotein; Mass spectrometry; Tandem affinity purification

Indexed keywords

BETA ACTIN; HOMEODOMAIN PROTEIN; PROTEIN MYB; PROTEOME; RNA POLYMERASE; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR PBX1;

AMINO ACID SEQUENCE; ANGIOGENESIS; ARTICLE; ENZYME ACTIVITY; HEMATOPOIESIS; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN ISOLATION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; WESTERN BLOTTING;

ANIMALS; BISBENZIMIDE; CELL NUCLEUS; CHROMATOGRAPHY, AFFINITY; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; FLUORESCENT DYES; GENE EXPRESSION REGULATION; GENES, REPORTER; GENETIC VECTORS; HOMEODOMAIN PROTEINS; LUCIFERASES; MASS SPECTROMETRY; MICE; NIH 3T3 CELLS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; PROTEIN SYNTHESIS INHIBITORS; PUROMYCIN; RETROVIRIDAE; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TRANSCRIPTION FACTORS; TRANSFECTION;

MUS;

EID: 34547877941     PISSN: 16159853     EISSN: 16159861     Source Type: Journal    
DOI: 10.1002/pmic.200700197     Document Type: Article

References (43)

1. Moens, C. B., Selleri, L., Hox cofactors in vertebrate development. Dev. Biol. 2006, 291, 193-206.
2. Ferretti, E., Villaescusa, J. C., Di Rosa, P., Fernandez-Diaz, L. C. et al., Hypomorphic mutation of the TALE gene Prep1 (pKnox1) causes a major reduction of Pbx and Meis proteins and a pleiotropic embryonic phenotype. Mol. Cell. Biol. 2006, 26, 5650-5662.
3. Ferretti, E., Marshall, H., Popperl, H., Maconochie, M. et al., Segmental expression of Hoxb2 in r4 requires two separate sites that integrate cooperative interactions between Prep1, Pbx and Hox proteins. Development 2000, 127, 155-166.
4. Ferretti, E., Cambronero, F., Tumpel, S., Longobardi, E. et al., Hoxb1 enhancer and control of rhombomere 4 expression: complex interplay between PREP1-PBX1-HOXB1 binding sites. Mol. Cell. Biol. 2005, 25, 8541-8552.
5. Penkov, D., Tanaka, S., Di Rocco, G., Berthelsen, J. et al., Cooperative interactions between PBX, PREP, and HOX proteins modulate the activity of the alpha 2(V) collagen (COL5A2) promoter. J. Biol. Chem. 2000, 275, 16681-16689.
6. Brendolan, A., Ferretti, E., Salsi, V., Moses, K. et al., A Pbx1-dependent genetic and transcriptional network regulates spleen ontogeny. Development 2005, 132, 3113-3126.
7. Rave-Harel, N., Givens, M. L., Nelson, S. B., Duong, H. A. et al., TALE homeodomain proteins regulate gonadotropin-releasing hormone gene expression independently and via interactions with Oct-1. J. Biol. Chem. 2004, 279, 30287-30297.
8. Bailey, J. S., rRave-Harel, N., McGillivray, S. M., Coss, D., Mellon, P. L., Activin regulation of the follicle-stimulating hormone beta-subunit gene involves Smads and the TALE homeodomain proteins Pbx1 and Prep1. Mol. Endocrinol. 2004, 18, 1158-1170.
9. Swift, G. H., Liu, Y., Rose, S. D., Bischof, L. J. et al., An endocrine-exocrine switch in the activity of the pancreatic homeodomain protein PDX1 through formation of a trimeric complex with PBX1b and MRG1 (MEIS2). Mol. Cell. Biol. 1998, 18, 5109-5120.
10. Goudet, G., Delhalle, S., Biemar, F., Martial, J. A., Peers, B., Functional and cooperative interactions between the homeodomain PDX1, Pbx, and Prep1 factors on the somatostatin promoter. J. Biol. Chem. 1999, 274, 4067-4073.
11. Huang, H., Paliouras, M., Rambaldi, I., Lasko, P., Featherstone, M., Nonmuscle myosin promotes cytoplasmic localization of PBX. Mol. Cell. Biol. 2003, 23, 3636-3645.
12. Abramovich, C., Shen, W. F., Pineault, N., Imren, S. et al., Functional cloning and characterization of a novel non-homeodomain protein that inhibits the binding of PBX1-HOX complexes to DNA. J. Biol. Chem. 2000, 275, 26172-26177.
13. Puig, O., Caspary, F., Rigaut, G., Rutz, B. et al., The tandem affinity purification (TAP) method: A general procedure of protein complex purification. Methods 2001, 24, 218-229.
14. Rigaut, G., Shevchenko, A., Rutz, B., Wilm, M. et al., A generic protein purification method for protein complex characterization and proteome exploration. Nat. Biotechnol. 1999, 17, 1030-1032.
15. Berthelsen, J., Kilstrup-Nielsen, C., Blasi, F., Mavilio, F., Zappavigna, V., The subcellular localization of PBX1 and EXD proteins depends on nuclear import and export signals and is modulated by association with PREP1 and HTH. Genes Dev. 1999, 13, 946-953.
16. Berthelsen, J., Zappavigna, V., Ferretti, E., Mavilio, F., Blasi, F., The novel homeoprotein Prep1 modulates Pbx-Hox protein cooperativity. EMBO. J. 1998, 17, 1434-1445.
17. Di Rocco, G., Mavilio, F., Zappavigna, V., Functional dissection of a transcriptionally active, target-specific Hox-Pbx complex. EMBO. J. 1997, 16, 3644-3654.
18. Morgenstern, J. P., Land, H., Advanced mammalian gene transfer: High titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line. Nucleic Acids Res. 1990, 18, 3587-3596.
19. Kinsella, T. M., Nolan, G. P., Episomal vectors rapidly and stably produce high-titer recombinant retrovirus. Hum Gene Ther. 1996, 7, 1405-1413.
20. Dignam, J. D., Lebovitz, R. M., Roeder, R. G., Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 1983, 11, 1475-1489.
21. Shevchenko, A., Wilm, M., Vorm, O., Mann, M., Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal. Chem. 1996, 68, 850-858.
22. Zhang, W., Chait, B. T., ProFound: An expert system for protein identification using mass spectrometric peptide mapping information. Anal. Chem. 2000, 72, 2482-2489.
23. Longobardi, E., Blasi, F., Overexpression of PREP-1 in F9 teratocarcinoma cells leads to a functionally relevant increase of PBX-2 by preventing its degradation. J. Biol. Chem. 2003, 278, 39235-39241.
24. Lu, Q., Kamps, M. P., Structural determinants within Pbx1 that mediate cooperative DNA binding with pentapeptide-containing Hox proteins: Proposal for a model of a Pbx1-Hox-DNA complex. Mol. Cell. Biol. 1996, 16, 1632-1640.
25. Kilstrup-Nielsen, C., Alessio, M., Zappavigna, V., PBX1 nuclear export is regulated independently of PBX-MEINOX interaction by PKA phosphorylation of the PBC-B domain. EMBO. J. 2003, 22, 89-99.
26. Saleh, M., Rambaldi, I., Yang, X. J., Featherstone, M. S., Cell signaling switches HOX-PBX complexes from repressors to activators of transcription mediated by histone deacetylases and histone acetyltransferases. Mol. Cell. Biol. 2000, 20, 8623-8633.
27. Keough, R., Woollatt, E., Crawford, J., Sutherland, G. R. et al., Molecular cloning and chromosomal mapping of the human homologue of MYB binding protein (P160) 1A (MYBBP1A) to 17p13.3. Genomics 1999, 62, 483-489.
28. Tavner, F. J., Simpson, R., Tashiro, S., Favier, D. et al., Molecular cloning reveals that the p160 Myb-binding protein is a novel, predominantly nucleolar protein which may play a role in transactivation by Myb. Mol. Cell. Biol. 1998, 18, 989-1002.
29. Obungu, V. H., Lee Burns, A., Agarwal, S. K., Chandrasekharapa, S. C. et al., Menin, a tumor suppressor, associates with nonmuscle myosin II-A heavy chain. Oncogene 2003, 22, 6347-6358.
30. Dreger, M., Bengtsson, L., Schoneberg, T., Otto, H., Hucho, F., Nuclear envelope proteomics: Novel integral membrane proteins of the inner nuclear membrane. Proc. Natl. Acad. Sci. USA 2001, 98, 11943-11948.
31. Wei, Q., Adelstein, R. S., Conditional expression of a truncated fragment of nonmuscle myosin II-A alters cell shape but not cytokinesis in HeLa cells. Mol. Biol. Cell. 2000, 11, 3617-3627.
32. Leal, A., Endele, S., Stengel, C., Huehne, K. et al., A novel myosin heavy chain gene in human chromosome 19q13.3. Gene 2003, 312, 165-171.
33. Hofmann, W. A., Stojiljkovic, L., Fuchsova, B., Vargas, G. M. et al., Actin is part of preinitiation complexes and is necessary for transcription by RNA polymerase II. Nat. Cell. Biol. 2004, 6, 1094-1101.
34. Bettinger, B. T., Gilbert, D. M., Amberg, D. C., Actin up in the nucleus. Nat. Rev. Mol. Cell. Biol. 2004, 5, 410-415.
35. Haller, K., Rambaldi, I., Daniels, E., Featherstone, M., Subcellular localization of multiple PREP2 isoforms is regulated by actin, tubulin, and nuclear export. J. Biol. Chem. 2004, 279, 49384-49394.
36. Kho, C. J., Wang, Y., Zarbl, H., Effect of decreased fte-1 gene expression on protein synthesis, cell growth, and transformation. Cell Growth Differ. 1996, 7, 1157-1166.
37. Lecomte, F., Szpirer, J., Szpirer, C., The S3a ribosomal protein gene is identical to the Fte-1 (v-fos transformation effector) gene and the TNF-alpha-induced TU-11 gene, and its transcript level is altered in transformed and tumor cells. Gene 1997, 186, 271-277.
38. Kashuba, E., Yurchenko, M., Szirak, K., Stahl, J. et al., Epstein-Barr virus-encoded EBNA-5 binds to Epstein-Barr virus-induced Fte1/S3a protein. Exp. Cell. Res. 2005, 303, 47-55.
39. Song, D., Sakamoto, S., Taniguchi, T., Inhibition of poly(-ADP-ribose) polymerase activity by Bcl-2 in association with the ribosomal protein S3a. Biochemistry 2002, 41, 929-934.
40. Cui, K., Coutts, M., Stahl, J., Sytkowski, A. J., Novel interaction between the transcription factor CHOP (GADD153) and the ribosomal protein FTE/S3a modulates erythropoiesis. J. Biol. Chem. 2000, 275, 7591-7596.
41. Wu, X., Yoo, Y., Okuhama, N. N., Tucker, P. W. et al., Regulation of RNA-polymerase-II-dependent transcription by N-WASP and its nuclear-binding partners. Nat. Cell. Biol. 2006, 8, 756-763.
42. Berthelsen, J., Zappavigna, V., Mavilio, F., Blasi, F., Prep1, a novel functional partner of Pbx proteins. EMBO. J. 1998, 17, 1423-1433.
43. Berthelsen, J., Vandekerkhove, J., Blasi, F., Purification and characterization of UEF3, a novel factor involved in the regulation of the urokinase and other AP-1 controlled promoters. J. Biol. Chem. 1996, 271, 3822-3830.