Highly chlorinated Escherichia coli cannot be stained by propidium iodide

Canadian Journal of Microbiology

Volumn 53, Issue 5, 2007, Pages 664-670

  Phe, M H ^a   Dossot, M ^a   Guilloteau, H ^a   Block, J C ^a  

^a Laboratoire de Chimie Physique et Microbiologie pour l'Environnement   (France)

Author keywords

Chlorination; Fluorescence; Membrane permeability; Nucleic acid staining; Propidium iodide; Water disinfection

Indexed keywords

BIOMOLECULES; CHLORINATION; CHLORINE; DNA; FLUORESCENCE; RNA; SUSPENSIONS (FLUIDS);

FLUOROCHROMES; MEMBRANE PERMEABILITY; PROPIDIUM IODIDE; SODIUM THIOSULPHATE; WATER DISINFECTION;

ESCHERICHIA COLI;

BACTERIAL DNA; BACTERIAL RNA; BLEACHING AGENT; CHLORINE; DEXTRAN; NUCLEIC ACID; PALMITIC ACID; PROPIDIUM IODIDE; SODIUM THIOSULFATE;

CHEMICAL SUBSTANCE; CHLORINATION; CHLORINE; COLIFORM BACTERIUM; DISINFECTION; MEMBRANE; NUCLEIC ACID; SULFATE;

ANIMAL CELL; ARTICLE; CELL MEMBRANE PERMEABILITY; CHLORINATION; CONTROLLED STUDY; ESCHERICHIA COLI; FLUORESCENCE ANALYSIS; NONHUMAN; PRIORITY JOURNAL; STAINING; SUSPENSION; VIRUS NEUTRALIZATION;

CELL MEMBRANE PERMEABILITY; CHLORINE; DISINFECTION; ESCHERICHIA COLI; PROPIDIUM; STAINING AND LABELING; WATER MICROBIOLOGY;

ESCHERICHIA COLI;

EID: 34547767192     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/W07-022     Document Type: Article

References (22)

1. Boulos, L., Prévost, M., Barbeau, B., Coallier, J., and Desjardins, R. 1999. LIVE/DEAD BacLight: application of a new rapid staining method for direct enumeration of viable and total bacteria in drinking water. J. Microbiol. Methods, 37: 77-86. doi:10.1016/S0167-7012(99)00048-2.
2. Caruso, G., Mancuso, M., and Crisafi, E. 2003. Combined fluorescent antibody assay and viability staining for the assessment of the physiological states of Escherichia coli in seawaters. J. Appl. Microbiol. 95: 225-233. doi:10.1046/j.1365-2672.2003.01967.x.
3. Garnerone, A., Rumeau, M., and Renau, M. 1982. Détermination du coefficient de transfert global d'un agent antiseptique à travers une membrane hydrophobe. Rev. Sci. Eau, 1: 31-39.
4. 2 photocatalysis involves adsorption onto catalyst and the loss of membrane integrity. FEMS Microbiol. Lett. 258: 18-24. doi:10.1111/j.1574-6968.2006.00190.x.
5. Grégon, G., Citterio, S., Ghiani, A., Labra, M., Sgorbati, S., Brown, S., and Denis, M. 2001. Resolution of viable and membrane-compromised bacteria in freshwater and marine waters based on analytical flow cytometry and nucleic acid double staining. Appl. Environ. Microbiol. 67: 4662-4610. doi:10.1128/AEM.67.10.4662-4670.2001.
6. Haugland, R.P. 2002. Handbook of fluorescent probes and research products. 9th ed. Molecular Probes Inc., Eugene, Ore.
7. Herrera, G., Martinez, A., Blanco, M., and O'Connor, J.E. 2002. Assessment of Escherichia coli B with enhanced permeability to fluorochromes for flow cytometric assays of bacterial cell function. Cytometry, 49: 62-69. doi:10.1002/cyto.10148.
8. Hoefel, D., Grooby, W.L., Monis, P.T., Andrews, S., and Saint, C.P. 2003. Enumeration of water-borne bacteria using viability assays and flow cytometry: a comparison to culture-based techniques. J. Microbiol. Methods, 55: 585-597. doi:10.1016/S0167-7012(03)00201-X.
9. Lopez-Amoros, R., Comas, J., and Vives-Rego, J. 1995. Flow cytometric assessment of Escherichia coli and Salmonella typhimurium starvation-survival in seawater using rhodamine 123, propidium iodide, and oxonol - physiological activities in bacterial biofilms using fluorescent probes. Appl. Environ. Microbiol. 61: 2521-2526.
10. Nebe-von-Caron, G., Stephens, P.J., Hewitt, C.J., Powell, J.R., and Badley, R.A. 2000. Analysis of bacterial function by multicolour fluorescence flow cytometry and single cell sorting. J. Microbiol. Methods, 42: 97-114. doi:10.1016/S0167-7012(00)00181-0.
11. Phe, M.H., Dossot, M., and Block, J.C. 2004. Chlorination effect on the fluorescence of nucleic acid staining dyes. Water Res. 38: 3729-3737. doi:10.1016/j.watres.2004.05.018.
12. Phe, M.H., Dossot, M., Guilloteau, H., and Block, J.C. 2005. Nucleic acid fluorochromes and flow cytometry prove useful in assessing the effect of chlorination on drinking water bacteria. Water Res. 39: 3618-3628. doi:10.1016/j.watres.2005.06.002.
13. Poncin, J. 1987. Recherche sur la chloration par l'hypochlorite de sodium de quelques substances organoazotées en milieux aqueux dilué. Ph.D. thesis, Faculté des sciences, Université de Rennes I, France. No. 482.
14. Prütz, W.A. 1999. Interactions of hypochlorous acid with pyrimidine nucleotides, and secondary reactions of chlorinated pyrimidines with GSH, NADH, and other substances. Arch. Biochem. Biophys. 349: 183-191.
15. Rodier, J. 1996. L'analyse de l'eau. 8e éd. Dunod, Paris.
16. Saby, S., Sibille, I., Mathieu, L., Paquin, J.L., and Block, J.C. 1997. Influence of water chlorination on the counting of bacteria with DAPI (4′,6-diamidino-2-phenylindole). Appl. Environ. Microbiol. 63: 1564-1569.
17. Stocks, S.M. 2004. Mechanism and use of the commercially available viability stain, BacLight. Cytometry A, 61: 189-195.
18. White, G.C. 1992. The handbook of chlorination and alternative disinfections. 3rd ed. Van Nostrand Reinhold, New York.
19. Whiteman, M., Jenner, A., and Halliwell, B. 1997. Hypochlorous acid-induced base modifications in isolated calf thymus DNA. Chem. Res. Toxicol. 10: 1240-1246. doi:10.1021/tx970086i.
20. Winterbourn, C.C. 1985. Comparative reactivities of various biological compounds with myeloperoxidase-hydrogen peroxide chloride, and similarity of the oxidant to hypochlorite. Biochim. Biophys. Acta, 840: 204-210.
21. Winterbourn, C.C., and Kettle, A.J. 2000. Biomarkers of myeloperoxidase-derived hypochlorous acid. Free Radic. Biol. Med. 29: 403-409. doi:10.1016/S0891-5849(00)00204-5.
22. Van, X., Grace, W.K., Yoshida, T.M., Habbersett, R.C., Velappan, N., Jett, J.H., Keller, R.A., and Marrone, L. 1999. Characteristics of different nucleic acid staining dyes for DNA fragment sizing by flow cytometry. Anal. Chem. 71: 5470-5480.