Identification of a novel laser dye substrate of mammalian cytochromes P450: Application in rapid kinetic analysis, inhibitor screening, and directed evolution

Journal of Biomolecular Screening

Volumn 12, Issue 5, 2007, Pages 677-682

  Kumar, Santosh ^a  

^a University of Texas Medical Branch School of Medicine   (United States)

Author keywords

Coumarin 152; Cytochrome P450; Directed evolution; Laser dye; P450 2C; Screening

Indexed keywords

7 DIMETHYLAMINO 4 TRIFLUOROMETHYLCOUMARIN; COUMARIN DERIVATIVE; CUMENE HYDROPEROXIDE; CYTOCHROME P450; CYTOCHROME P450 2B1; CYTOCHROME P450 2C19; CYTOCHROME P450 2C8; CYTOCHROME P450 2C9; CYTOCHROME P450 3A4; CYTOCHROME P450 3A5; CYTOCHROME P450 INHIBITOR; FLUORESCENT DYE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG;

ARTICLE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; EVOLUTION; HIGH THROUGHPUT SCREENING; LASER; MAMMAL; PRIORITY JOURNAL;

ANIMALS; BENZENE DERIVATIVES; BUFFERS; COLORING AGENTS; COUMARINS; CYTOCHROME P-450 ENZYME SYSTEM; DIRECTED MOLECULAR EVOLUTION; HUMANS; HYDROGEN PEROXIDE; HYDROGEN-ION CONCENTRATION; KINETICS; LASERS; LIVER; MAMMALS; OXIDANTS; OXIDATION-REDUCTION; RATS; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

MAMMALIA;

EID: 34547748897     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057107301496     Document Type: Article

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