Mutation and a high-throughput screening method for improving the production of Epothilones of Sorangium

Journal of Industrial Microbiology and Biotechnology

Volumn 34, Issue 9, 2007, Pages 615-623

  Gong, Guo Li ^a   Sun, Xin ^a   Liu, Xin Li ^a   Hu, Wei ^a   Cao, Wen Rui ^a   Liu, Hong ^a   Liu, Wei Feng ^a   Li, Yue Zhong ^a  

^a SHANDONG UNIVERSITY   (China)

Author keywords

Epothilone; Genome shuffling; High throughput screening; Sorangium cellulosum

Indexed keywords

EPOTHILONE DERIVATIVE;

ARTICLE; BACTERIAL STRAIN; HIGH THROUGHPUT SCREENING; MUTATION; MYCOBACTERIUM; NONHUMAN; PHENOTYPE; SORANGIUM; TITRIMETRY; ULTRAVIOLET RADIATION;

ANTINEOPLASTIC AGENTS; DRUG SCREENING ASSAYS, ANTITUMOR; EPOTHILONES; EPOXY COMPOUNDS; MUTATION; MYXOCOCCALES;

POLYANGIUM CELLULOSUM; SORANGIUM;

EID: 34547654448     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-007-0236-2     Document Type: Article

References (34)

1. Höfle G, Bedorf N, Gerth K, Reichenbach H (1993) Epothilone, dere Herstellungsverfahren sowie sie enthaltende Mittel. DE 4138042 A1, published May 27 (priority Nov. 19, 1991)
2. Gerth K, Bedorf N, Höfle G, Irschik H, Reichenbach H (1996) Epothilons A and B: Antifungal and cytotoxic compounds from Sorangium cellulosum (Myxobacteria)-production, physico-chemical and biological properties. J Antibiot 49:560-564
3. Bollag DM, McQueney PA, Zhu J, Hensens O, Koupal L, Liesch J, Goetz M, Lazarides E, Woods CM (1995) Epothilones, a new class of microtubule-stabilizing agents with a taxol-like mechanism of action. Cancer Res 55:2325-2333
4. Larkin JM, Kaye SB (2006) Epothilones in the treatment of cancer. Expert Opin Investig Drugs 15:691-702
5. Julien B, Shah S (2002) Heterogonous expression of epothilone biosynthetic genes in Myxococcus xanthus. Antimicrob Agents Chemother 46:2772-2778
6. Tang L, Shah S, Chung L, Carney J, Katz L, Khosla C, Julien B (2000) Cloning and heterologous expression of the epothilone gene cluster. Science 287:640-642
7. Mutka SC, Carney JR, Liu YQ, Kennedy J (2006) Heterologous Production of Epothilone C and D in Escherichia coli. Biochemistry 45:1321-1330
8. Lau J, Frykman S, Regentin R, Qu S (2002) Optimizing the heterologous production of Epothilone D in Myxococcus xanthus. Biotechnol Bioeng 78:280-288
9. Frykman SA, Tsuruta H, Lacari PJ (2005) Assessment of fed-batch, semicontinuous, and continuous Epothilone D production processes. Biotechnol Prog 21:1102-1108
10. Reichenbach H (2001) Myxobacteria, producers of novel bioactive substances. J Ind Microbiol Biotechnol 27:149-156
11. Gerth K, Pradella S, Perlova O, Beyer S, Müller R (2003) Myxobacteria: proficient producers of novel natural products with various biological activities -past and future biotechnological aspects with the focus on the genus Sorangium. J Biotechnol 106:233-253
12. Reichenbach H, Dworkin M (1992) The Myxobacteria. In: Balows A, Trüper GH, Dworkin M, Harder W, Schleifer KH (Eds) The prokaryotes, vol 2. Springer, New York, pp 3416-3487
13. Jaoua S, Neff S, Schupp T (1992) Transfer of mobilizable plasmids to Sorangium cellulosum and evidence for their integration into the chromosome. Plasmid 28:157-165
14. Julien B, Fehd R (2003) Development of a mariner-based transposon for use in Sorangium cellulosum. Appl Environ Microbiol 69:6299-6301
15. Kopp M, Irschik H, Gross F, Perlova O, Sandmanna A, Gerth K, Müller R (2004) Critical variations of conjugational DNA transfer into secondary metabolite multiproducing Sorangium cellulosum strains So ce12 and So ce56: development of a mariner-based transposon mutagenesis system. J Biotechnol 107:29-40
16. Zirkle R, Ligon JM, Molnár I (2004) Heterologous production of the antifungal polyketide antibiotic soraphen A of Sorangium cellulosum So ce26 in Streptomyces lividans. Microbiology 150:2761-2774
17. Bode BH, Müller R (2006) Analysis of myxobacterial secondary metabolism goes molecular. J Ind Microbiol Biotechnol 33:577-588
18. Zhang YX, Perry K, Vinci VA, Powell K, Stemmer WP, Cardayre SB (2002) Genome shuffling leads to rapid phenotypic improvement in bacteria. Nature 415:644-646
19. Stephanopoulos G (2002) Metabolic engineering by genome shuffling. Nat Biotechnol 20:660-668
20. Patnaik R, Louie S, Gavrilovic V, Perry K, Stemmer WPC, Ryan CM, del Cardayre S (2002) Genome shuffling of Lactobacillus for improved acid tolerance. Nat Biotechnol 20:707-712
21. Dai MH, Copley SD (2004) Genome shuffling improves degradation of the anthropogenic pesticide pentachlorophenol by Sphingobium chlorophenolicum ATCC 39723. Appl Environ Microbiol 70:2391-2397
22. Dong H, Li YZ, Hu W (2004) Analysis of purified tubulin in high concentration of glutamate for application in high throughput screening for microtubule-stabilizing agents. Assay Drug Dev Technol 2:621-628
23. Li ZF, Zhao JY, Xia ZJ, Shi J, Liu H, Wu ZH, Hu W, Liu WF, Li YZ (2007) Evolutionary diversity of ketoacyl syntheses in cellulolytic myxobacterium Sorangium. Syst Appl Microbiol 30:189-196
24. Nguimbi E, Li YZ, Gao BL, Li ZF, Wang B, Wu ZH, Yan BX, Qu YB, Gao PJ (2003) 16S-23S ribosomal DNA intergenic spacer regions in cellulolytic myxobacteria and differentiation of closely related strains. Syst Appl Microbiol 26:262-268
25. Regentin R, Frykman S, Tsuruta H, Licari P (2003) Nutrient regulation of Epothilone biosynthesis in heterologous and native production strains. Appl Microbiol Biotechnol 61:451-455
26. Gerth K, Steinrich H, Hofle G, Reichenbach H (2000) Studies on the biosynthesis of epothilones: the biosynthetic origin of the carbon skeleton. J Antibiot 53:1373-1377
27. Gerth K, Steinmetz H, Höfle G, Reichenbach H (2001) Studies on the biosynthesis of epothilones: the PKS and Epothilone C/D monooxygenase. J Antibiot 54:144-148
28. Wyrick PB, Rogers HJ (1973) Isolation and characterization of cell wall-defective variants of Bacillus subtilis and Bacillus licheniformis. J Bacteriol 116:456-465
29. Xu ZN, Shen WH, Chen XY, Lin JP, Cen PL (2005) A high-throughput method for screening of rapamycin-producing strains of Streptomyces hygroscopicus by cultivation in 96-well microtiter plates. Biotechnol Lett 27:1135-1140
30. Jin ZH, Lin JP, Xu ZN, Cen PL (2002) Improvement of industry-applied rifamycin B-producing strain, Amycolatopsis mediterranei, by rational screening. J Gen Appl Microbiol 48:329-334
31. Gravius B, Glocker D, Pigac J, Pandza K, Hranueli D, Cullum J (1994) The 387 kb linear plasmid pPZG101 of Streptomyces rimosus and its interactions with the chromosome. Microbiology 140:2271-2277
32. Adrio1 JL, Demain AL (2006) Genetic improvement of processes yielding microbial products. FEMS Microbiol Rev 30:187-214
33. Abe M, Nakai T, Umetsu H, Sasage D (1984) Regeneration of mycelial protoplasts from Lyophilum shimeji. Agic Biol Chem 48(6):1635-1636
34. Shirai M, Okada Y, Aida T (1985) Conversion of protoplasts of colistin-producing forms. Agric Biol Chem 49(3):595-560