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Volumn 76, Issue 1, 2007, Pages

Dynamic and static light scattering analysis of DNA ejection from the phage λ

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIOPHAGES; DNA SEQUENCES; ELEMENTARY PARTICLES; RELAXATION TIME;

EID: 34547410763     PISSN: 15393755     EISSN: 15502376     Source Type: Journal    
DOI: 10.1103/PhysRevE.76.011914     Document Type: Article
Times cited : (16)

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    • This fact, in addition to the low concentration, explains why no relaxation process of the free DNA is observed in the DLS measurements on the phage system. Attempts were also made to measure DLS on pure λ -DNA in bulk solution with the same concentration as the ejected DNA in our phage system but the scattering intensity was too low, confirming our assumption above. Even an attempt to increase DNA concentration by 100 times and decrease to θ=35° was unsuccessful.
    • This fact, in addition to the low concentration, explains why no relaxation process of the free DNA is observed in the DLS measurements on the phage system. Attempts were also made to measure DLS on pure λ -DNA in bulk solution with the same concentration as the ejected DNA in our phage system but the scattering intensity was too low, confirming our assumption above. Even an attempt to increase DNA concentration by 100 times and decrease to θ=35° was unsuccessful.


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