Comparison of several linear fluorophore- and quencher-conjugated oligomer duplexes for stability, fluorescence quenching, and kinetics in vitro and in vivo in mice

Bioconjugate Chemistry

Volumn 18, Issue 4, 2007, Pages 1170-1175

  Zhang, Surong ^a   Liu, Guozheng ^a   Liu, Xinrong ^a   Yin, Dongguang ^a   Dou, Shuping ^a   He, Jiang ^a,b   Rusckowski, Mary ^a   Hnatowich, Donald J ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BODY FLUIDS; DNA; FLUORESCENCE QUENCHING; FLUOROPHORES; KINETICS; MICROSPHERES; OPTICAL PROPERTIES;

CONJUGATED OLIGOMER; DETECTABLE SIGNAL; FLUORESCENCE KINETICS; FLUORESCENCE QUENCHING; IN-VITRO; LINEAR OLIGOMERS; PHOSPHODIESTERS; PHOSPHOROTHIOATES; USEFUL PROPERTIES; VITRO AND IN VIVO;

MAMMALS;

BUFFER; COMPLEMENTARY DNA; ESTER DERIVATIVE; MICROSPHERE; MORPHOLINE DERIVATIVE; OLIGOMER; PHOSPHOROTHIOIC ACID; PHOSPHORUS DERIVATIVE;

ANIMAL EXPERIMENT; ARTICLE; COMPARATIVE STUDY; CONJUGATION; CONTROLLED STUDY; FEASIBILITY STUDY; FLUORESCENCE; IMAGING SYSTEM; IN VITRO STUDY; IN VIVO STUDY; KINETICS; MALE; MOLECULAR STABILITY; MOUSE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS;

MUS;

EID: 34547193651     PISSN: 10431802     EISSN: None     Source Type: Journal    
DOI: 10.1021/bc070021j     Document Type: Article

References (21)

1. Marras, S. A., Kramer, F. R., and Tyagi, S. (2002) Efficiencies of fluorescence resonance energy transfer and contact-mediated quenching in oligonucleotide probes. Nucleic Acids Res. 30, e122.
2. Liu, G., He, J., Dou, S., Gupta, S., Rusckowski, M., and Hnatowich, D. J. (2005) Further investigations of morpholino pretargeting in mice - establishing quantitative relations in tumor. Eur. J. Nucl. Med. 32, 1115-1123.
3. He, J., Liu, X., Zhang, S., Liu, G., and Hnatowich, D. J. (2005) Affinity enhancement bivalent morpholino for pretargeting: surface plasmon resonance studies of molecular dimensions. Bioconjugate Chem. 16, 1098-1104.
4. Liu, X., Nakamura, K., Wang, Y., Wang, Y., Liu, G., He, J., Ding, H., Lu, P., Rusckowski, M., Kubo, A., and Hnatowich, D. J. (2006) Initial mechanistic studies of antisense targeting in cells. J. Nucl. Med. 47, 360-368.
5. Tyagi, S., and Kramer, F. R. (1996) Molecular beacons: probes that fluoresce upon hybridization. Nat. Biotechnol. 14, 303-308.
6. Silverman, A. P., and Kool, E. T. (2005) Quenched probes for highly specific detection of cellular RNAs. Trends in Biotechnol. 23, 225-230.
7. Nitin, N., Santangelo, P. J., Kim, G., Nie, S., and Bao, G. (2004) Peptide-linked molecular beacons for efficient delivery and rapid mRNA detection in living cells. Nucleic Acids Res. 32, e58.
8. McIntyre, J. O., Fingleton, B., Wells, K. S., Piston, D. W., Lynch, C. C., Gautam, S., and Matrisian, L. M. (2004) Development of a novel fluorogenic proteolytic beacon for in vivo detection and imaging of tumour-associated matrix metalloproteinase-7 activity. Biochem. J. 377, 617-628.
9. Hoeflich, K. P., Gray, D. C., Eby, M. T., Tien, J. Y., Wong, L., Bower, J., Gogineni, A., Zha, J., Cole, M. J., Stem, H. M., Murray, L. J., Davis, D. P., and Seshagiri, S. (2006) Oncogenic BRAF is required for tumor growth and maintenance in melanoma models. Cancer Res. 66, 999-1006.
10. Molenaar, C., Marras, S. A., Slats, J. C., Truffert, J. C., Lemaitre, M., Raap, A. K., Dirks, R. W., and Tanke, H. J. (2001) Linear 2′ O-Methyl RNA probes for the visualization of RNA in living cells. Nucleic Acids Res. 29, E89-9.
11. Tyagi, S., and Alsmadi, O. (2004) Imaging Native beta-Actin mRNA in motile fibroblasts. Biophys. J. 87, 4153-4162.
12. He, J., Rusckowski, M., Wang, Y., Dou, S., Liu, X., Zhang, S., Liu, G., and Hnatowich, D. J. (2007) Optical pretargeting of tumor with fluorescent MORF oligomers. Mol. Imaging Biol. 9, 17-23.
13. Liu, G., He, J., Dou, S., Gupta, S., Vanderheyden, J. L., Rusckowski, M., and Hnatowich, D. J. (2004) Pretargeting in tumored mice with radiolabeled morpholino oligomer showing low kidney uptake. Eur. J. Nucl. Med. Mol. Imaging 31, 417-424.
14. Zhang, Y., He, J., Liu, G., Vanderheyden, J. L., Gupta, S., Rusckowski, M., and Hnatowich, D. J. (2004) Initial Observations of 99mTc-labeled Locked Nucleic Acids for Antisense Targeting. Nucl. Med. Commun. 25, 1113-1116.
15. Moreira, B. G., You, Y., Behlke, M. A., and Owczarzy, R. (2005) Effects of fluorescent dyes, quenchers, and dangling ends on DNA duplex stability. Biochem. Biophys. Res. Commun. 327, 473-484.
16. Weissleder, R., Tung, C. H., Mahmood, U., and Bogdanov, A. Jr. (1999) In vivo imaging of tumors with protease-activated near-infrared fluorescent probes. Nat. Biotechnol. 17, 375-378.
17. Petrovsky, A., Schellenberger, E., Josephson, L., Weissleder, R., and Bogdanov, A. Jr. (2003) Nearinfrared fluorescent imaging of tumor apoptosis. Cancer Res. 63, 1936-1942.
18. Ke, S., Wen, X., Gurfinkel, M., Charnsangavej, C., Wallace, S., Sevick-Muraca, E. M., and Li, C. (2003) Near-infrared optical imaging of epidermal growth factor receptor in breast cancer xenografts. Cancer Res. 63, 7870-7875.
19. Chen, X., Conti, P. S., and Moats, R. A. (2004) In vivo near-infrared fluorescence imaging of integrin alphavbeta3 in brain tumor xenografts. Cancer Res. 64, 8009-8014.
20. Tidd, D. M., and Warenius, H. M. (1989) Partial protection of oncogene, anti-sense oligodeoxynucleotides against serum nuclease degradation using terminal methylphosphonate groups. Br. J. Cancer 60, 343-350.
21. Shaw, J. P., Kent, K., Bird, J., Fishback, J., and Froehler, B. (1991) Modified deoxyoligonucleotides stable to exonuclease degradation in serum. Nucleic Acids Res. 19, 747-750.