Evaluation of a real-time PCR assay based on the repetitive B1 gene for the detection of Toxoplasma gondii in human peripheral blood

Parasitology Research

Volumn 101, Issue 3, 2007, Pages 619-625

  Kompalic Cristo, Alicia ^a,b   Frotta, Cassia ^c   Suárez Mutis, Martha ^a   Fernandes, Octavio ^a,c   Britto, Constança ^a  

^a INSTITUTO OSWALDO CRUZ   (Brazil)

^b UNIVERSIDAD CENTROCCIDENTAL LISANDRO ALVARADO   (Venezuela)

^c Nucleo Tecnico Operacional   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

B1 PROTEIN; BETA ACTIN; DNA; GENE PRODUCT; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BLOOD; CLINICAL ARTICLE; CONTROLLED STUDY; DISEASE ASSOCIATION; DNA EXTRACTION; FEMALE; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENE AMPLIFICATION; GENOME; HUMAN; HYBRIDIZATION; MOUSE; NONHUMAN; OPTICAL DENSITY; PARASITE IDENTIFICATION; PARASITEMIA; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SERODIAGNOSIS; SEROLOGY; TACHYZOITE; TOXOPLASMA GONDII; TOXOPLASMOSIS;

TOXOPLASMA; TOXOPLASMA GONDII;

EID: 34547138879     PISSN: 09320113     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00436-007-0524-9     Document Type: Article

References (31)

1. Bastien P (2002) Molecular diagnosis of toxoplasmosis. Trans R Soc Trop Med Hyg 96:S205-S215
2. Bretagne S, Costa JM, Fleury-Feith J, Poron F, Dubreuil-Lemaire ML, Vidaud M (1995) Quantitative competitive PCR with bronchoalveolar lavage fluid for diagnosis of toxoplasmosis in AIDS patients. J Clin Microbiol 33:1662-1664
3. Buchbinder S, Blatz R, Rodloff AC (2003) Comparison of real-time PCR detection methods for B1 and P30 genes of Toxoplasma gondii. Diagn Microbiol Infect Dis 45:269-271
4. Burg JL, Grover CM, Pouletty P, Boothroyd JC (1989) Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction. J Clin Microbiol 27:1787-1792
5. Cantos GA, Prando MD, Siqueira MV, Teixeira RM (2000) Toxoplasmose: Ocorrência de anticorpos anti-Toxoplasma gondii e diagnóstico. Rev Assoc Med Bras 46:335-341
6. Cassaing S, Bessières MH, Berry A, Berrebi A, Fabre R, Magnaval JF (2006) Comparison between two amplification sets for molecular diagnosis of toxoplasmosis by real-time PCR. J Clin Microbiol 44:720-724
7. Coelho RAI, Kobayashi M, Carvalho LB Jr (2003) Prevalence of IgG antibodies specific to Toxoplasma gondii among blood donors in Recife, Northeast Brazil. Rev Inst Med Trop Sao Paulo 45:229-231
8. Contini C, Seraceni S, Cultrera R (1999) Different PCR systems to detect Toxoplasma gondii tachyzoites or bradyzoites in clinical specimens from patients with and without overt disease. J Eukaryot Microbiol 46:S77-S78
9. Contini C, Cultrera R, Seraceni S, Segala D, Romani R, Fainardi E, Cinque P, Lazzarin A, Delia S (2002) The role of stage-specific oligonucleotide primers in providing effective laboratory support for the molecular diagnosis of reactivates Toxoplasma gondii encephalitis in patients with AIDS. J Med Microbiol 51:879-890
10. Costa JM, Pautas C, Ernault P, Foulet F, Cordonnier C, Bretagne S (2000) Real-time PCR for diagnosis and follow-up of Toxoplasma reactivation after allogeneic stem cell transplantation using fluorescence resonance energy transfer hybridization probes. J Clin Microbiol 38:2929-2932
11. Costa JM, Ernault P, Gautier E, Bretagne S (2001) Prenatal diagnosis of congenital toxoplasmosis by duplex real-time PCR using fluorescence resonance energy transfer hybridization probes. Prenat Diagn 21:85-88
12. Grob U, Roggenkamp A, Janitschke K, Heesemann J (1992) Improved sensitivity of the polymerase chain reaction for detection of Toxoplasma gondii in biological and human clinical specimens. Eur J Clin Microbiol Infect Dis 11:33-39
13. Guy EC, Joynson HM (1995) Potential of the polymerase chain reaction in the diagnosis of active Toxoplasma infection by detection of parasite in blood. J Infect Dis 172:319-322
14. Hierl T, Reischl U, Lang P, Hebart H, Stark M, Kyme P, Autenrieth IB (2004) Preliminary evaluation of one conventional nested and two real time PCR assays for the detection of Toxoplasma gondii in immunocompromised patients. J Med Microbiol 53:1-4
15. Hitt JA, Filice GA (1992) Detection of Toxoplasma gondii parasitemia by gene amplification, cell culture, and mouse inoculation. J Clin Microbiol 30:3181-3184
16. Ho-Yen DO, Joss AW, Balfour AH, Smyth ETM, Baird D, Chatterton JMW (1992) Use of the polymerase chain reaction to detect Toxoplasma gondii in human blood samples. J Clin Pathol 45:910-913
17. James GS, Sintchenko VG, Dickeson DJ, Gilbert GL (1996) Comparison of cell culture, mouse inoculation, and PCR for detection of Toxoplasma gondii: effects of storage conditions on sensitivity. J Clin Microbiol 34:1572-1575
18. Johnson JD, Butcher PD, Savva D, Holliman RE (1993) Application of the polymerase chain reaction to the diagnosis of human toxoplasmosis. J Infect 26:147-158
19. Khoury H, Adkins D, Brown R, Goodnough L, Gokden M, Roberts T, Storch G, DiPersio J (1999) Successful treatment of cerebral toxoplasmosis in a marrow transplant recipient: contribution of a PCR test in diagnosis and early detection. Bone Marrow Transplant 14:409-411
20. Kompalic-Cristo A, Nogueira SA, Guedes AL, Frota C, González LF, Brandão A, Amendoeira MR, Britto C, Fernandes O (2004) Lack of technical specificity in the molecular diagnosis of toxoplasmosis. Trans R Soc Trop Med Hyg 98:92-95
21. Martino R, Bretagne S, Einsele H, Maertens J, Ullmann AJ, Parody R, Schumacher U, Pautas C, Theunissen K, Schindel C, Munoz C, Margali N, Cordonnier C (2005) Early detection of Toxoplasma infection by molecular monitoring of Toxoplasma gondii in peripheral blood samples after allogeneic stem cell transplantation. Clin Infect Dis 40:67-78
22. Mei-Hui L, Tse-Ching C, Tseng-Tong K, Ching-Chung T, Ching-Ping T (2000) Real-Time PCR for quantitative detection of Toxoplasma gondii. J Clin Microbiol 38:4121-4125
23. Montoya JG (2002) Laboratory diagnosis of Toxoplasma gondii infection and toxoplasmosis. J Infect Dis 185:S73-S82
24. O'Driscoll JC, Holliman RE (1991) Toxoplasmosis and bone marrow transplantation. Rev Med Microbiol 2:215-222
25. Pelloux H, Weiss J, Simon J, Muet F, Fricker-Hidalgo H, Goullier-Fleuret A, Ambroise-Thomas P (1996) A new set of primers for the detection of Toxoplasma gondii in amniotic fluid using polymerase chain reaction. FEMS Microbiol Lett 138:11-15
26. Pelloux H, Dupoy-Camet J, Derouin F, Aboulker JP, Raffi F, the Bio-Toxo Study Group (1997) A multicentre prospective study for the polymerase chain reaction detection of Toxoplasma gondii DNA in blood samples from 186 AIDS patients with suspected toxoplasmic encephalitis. AIDS 11:1888-1890
27. Reischl U, Bretagne S, Krüger D, Ernault P, Costa JM (2003) Comparison of two DNA targets for the diagnosis of toxoplasmosis by real-time PCR using fluorescence resonance energy transfer hybridization probes. BMC Infect Dis 3:7-15
28. Remington J, Thulliez P, Montoya JG (2004) Recent developments for diagnosis of toxoplasmosis. J Clin Microbiol 42:941-945
29. Romand S, Chosson M, Franck J, Wallon M, Kieffer F, Kaiser K, Dumon H, Peyron F, Thulliez P, Picot S (2004) Usefulness of quantitative polymerase chain reaction in amniotic fluid as early prognostic marker of fetal infection with Toxoplasma gondii. Am J Obstet Gynecol 190:797-802
30. Simon A, Labalette P, Ordinaire I, Fréalle E, Dei-Cas E, Camus D, Delhaes L (2004) Use of fluorescence resonance energy transfer hybridization probes to evaluate quantitative real-time PCR for diagnosis of ocular toxoplasmosis. J Clin Microbiol 42:3681-3685
31. Wong SY, Remington JS (1993) Biology of Toxoplasma gondii. AIDS 7:299-316