Application of real-time RT-PCR for the quantitation and competitive replication study of H5 and H7 subtype avian influenza virus

Journal of Virological Methods

Volumn 119, Issue 2, 2004, Pages 151-158

  Lee, Chang Won ^a   Suarez, David L ^a  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

Avian influenza virus; Quantification; Real time RT PCR; Virus titration

Indexed keywords

ANALYTIC METHOD; ARTICLE; HYDROLYSIS; INFLUENZA VIRUS; MOLECULAR PROBE; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRUS ISOLATION; VIRUS REPLICATION; VIRUS TITRATION; VIRUS TYPING;

ANIMALS; FLUORESCENT DYES; HEMAGGLUTININ GLYCOPROTEINS, INFLUENZA VIRUS; INFLUENZA A VIRUS; POULTRY; POULTRY DISEASES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VIRUS REPLICATION;

AVES; AVIAN INFLUENZA VIRUS; GALLUS GALLUS; INFLUENZA VIRUS;

EID: 2442706274     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2004.03.014     Document Type: Article

References (23)

1. Beard, C.W., 1989. Serological procedures. In: A Laboratory Manual for the Isolation and Identification of Avian Pathotypes. Kendall-Hunt Publishing, Dubuque, pp. 192-200.
2. Carli K.T., Eyigor A. Real-time polymerase chain reaction for Mycoplasma gallisepticum in chicken trachea. Avian Dis. 47:2003;712-717
3. Fichtner, G.J., 1987. The Pennsylvania/Virginia experience in eradication of avian influenza (H5N2). In: Beard, C.W., Easterday, B.C. (Eds.), Proceedings of the Second International Symposium on Avian Influenza. US Animal Health Association, Richmond, VA, pp. 33-38.
4. Gut M., Leutenegger C.M., Huder J.B., Pedersen N.C., Lutz H. One-tube fluorogenic reverse transcription-polymerase chain reaction for the quantitation of feline coronaviruses. J. Virol. Methods. 77:1999;37-46
5. Horimoto T., Kawaoka Y. Molecular changes in virulent mutants arising from avirulent avian influenza viruses during replication in 14-day-old embryonated eggs. Virology. 206:1995;755-759
6. Jackwood D.J., Sommer S.E. Identification of infectious bursal disease virus quasispecies in commercial vaccines and field isolates of this double-stranded RNA virus. Virology. 304:2002;105-113
7. Jackwood M.W., Hilt D.A., Callison S.A. Detection of infectious bronchitis virus by real-time reverse transcriptase-polymerase chain reaction and identification of a quasispecies in the Beaudette strain. Avian Dis. 47:2003;718-724
8. Kim Y., Gharaibeh S.M., Stedman N.L., Brown T.P. Comparison and verification of quantitative competitive reverse transcription polymerase chain reaction (QC-RT-PCR) and real time RT-PCR for avian leukosis virus subgroup J. J. Virol. Methods. 102:2002;1-8
9. Lasley, F.A., 1987. Economics of avian influenza: control vs. noncontrol. In: Beard, C.W., Easterday, B.C. (Eds.), Proceedings of the Second International Symposium on Avian Influenza. US Animal Health Association, Richmond, VA, pp. 390-399.
10. Lee, C.W., Senne, D.A., Linares, J.A., Woolcock, P., Stallknecht, D., Spackman, E., Swayne, D., Suarez, D.L., in press-a. Characterization of recent H5 subtype avian influenza viruses from US poultry. Avian Pathol.
11. Lee, C.W., Senne, D.A. Suarez, D.L., in press-b. Generation of reassortant influenza vaccines by reverse genetics that allows utilization of a DIVA (differentiating infected from vaccinated animals) strategy for the control of avian influenza. Vaccine.
12. Lee, C.W., Senne, D.A., Suarez, D.L., in press-c. Effect of vaccine use in the evolution of Mexican-lineage H5N2 avian influenza virus. J. Virol.
13. Panigrahy B., Senne D.A., Pedersen J.C. Avian influenza virus subtypes inside and outside the live bird markets, 1993-2000: a spatial and temporal relationship. Avian Dis. 46:2002;298-307
14. Reed L.J., Muench H. A simple method for estimating fifty percent endpoints. Am. J. Hyg. 27:1938;493-497
15. Senne, D.A., Pearson, J.E., Panigrahy, B., 1992. Live poultry markets: a missing link in the epidemiology of avian influenza. In: Easterday, B.C. (Ed.), Proceedings of the Third International Symposium on Avian Influenza. University of Wisconsin, Madison, WI, pp. 50-58.
16. Senne D.A., Panigrahy B., Kawaoka Y., Pearson J.E., Suss J., Lipkind M., Kida H., Webster R.G. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 40:1996;425-437
17. Spackman E., Senne D.A., Davison S., Suarez D.L. Sequence analysis of recent H7 avian influenza viruses associated with three different outbreaks of commercial poultry in the US. J. Virol. 77:2003;13399-13402
18. Spackman E., Senne D.A., Myers T.J., Bulaga L.L., Garber L.P., Perdue M.L., Lohman K., Daum L.T., Suarez D.L. Development of a real-time reverse transcriptase PCR assay for type A influenza virus and the avian H5 and H7 hemagglutinin subtypes. J. Clin. Microbiol. 40:2002;3256-3260
19. Suarez D.L., Spackman E., Senne D.A. Update on molecular epidemiology of H1, H5, and H7 influenza virus infections in poultry in North America. Avian Dis. 47:2003;888-897
20. Suarez D.L., Garcia M., Latimer J., Senne D.A., Perdue M. Phylogenetic analysis of H7 avian influenza viruses isolated from the live bird markets of the Northeast United States. J. Virol. 73:1999;3567-3573
21. Suarez D.L., Senne D.A. Sequence analysis of related low-pathogenic and highly pathogenic H5N2 avian influenza isolates from United States live bird markets and poultry farms from 1983 to 1989. Avian Dis. 44:2000;356-364
22. Swayne, D.E., Halvorson, D.A., 2003. Influenza. In: Saif, Y.M., Barnes, H.J., Glisson, J.R., Fadly, A.M., McDougald, L.R., Swayne, D.E. (Eds.), Diseases of Poultry. Iowa State University Press, Ames, IA, pp. 135-160.
23. Villegas, P., 1998. Titration of biological suspensions. In: Swayne, D.E. (Ed.), A Laboratory Manual for the Isolation and Identification of Avian Pathogens, fourth ed. American Association of Avian Pathologists, Kennett Square, PA, pp. 248-254.