Erythroyte aspartate aminotransferase activity as a possible indirect marker for stimulated erythropoiesis in male and female athletes

Laboratory Hematology

Volumn 13, Issue 2, 2007, Pages 49-55

  Robinson, Yohan ^a   Cristancho, Edgar ^b   Böning, Dieter ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^b UNIVERSIDAD NACIONAL DE COLOMBIA   (Colombia)

Author keywords

Doping; EPO; Erythropoiesis; Red blood cells; Sports

Indexed keywords

ASPARTATE AMINOTRANSFERASE; ERYTHROCYTE ENZYME; ERYTHROPOIETIN; RECOMBINANT ERYTHROPOIETIN; BIOLOGICAL MARKER;

ADULT; AGED; ALTITUDE; ARTICLE; ASPARTATE AMINOTRANSFERASE BLOOD LEVEL; ATHLETE; CELL ACTIVATION; CLINICAL ARTICLE; CONCENTRATION RESPONSE; CONFIDENCE INTERVAL; CONTROLLED STUDY; DOPING; ENZYME ACTIVITY; ERYTHROPOIESIS; FEMALE; GEOGRAPHIC DISTRIBUTION; HUMAN; KIDNEY FAILURE; MALE; OXYGEN CONSUMPTION; PROTEIN BLOOD LEVEL; RESIDENTIAL HOME; SENSITIVITY ANALYSIS; STATISTICAL SIGNIFICANCE; TRAINING; ACCLIMATIZATION; ADOLESCENT; ANEMIA; BLOOD; CHRONIC KIDNEY FAILURE; DRUG EFFECT; ENZYMOLOGY; ERYTHROCYTE; EXERCISE TEST; METHODOLOGY; PHYSIOLOGY; PREDICTION AND FORECASTING; SEXUAL DEVELOPMENT; SPORTS MEDICINE; VALIDATION STUDY;

ACCLIMATIZATION; ADOLESCENT; ADULT; ALTITUDE; ANEMIA; ASPARTATE AMINOTRANSFERASES; BIOLOGICAL MARKERS; DOPING IN SPORTS; ERYTHROCYTES; ERYTHROPOIESIS; ERYTHROPOIETIN, RECOMBINANT; EXERCISE TEST; FEMALE; HUMANS; KIDNEY FAILURE, CHRONIC; MALE; PREDICTIVE VALUE OF TESTS; SEX CHARACTERISTICS; SPORTS MEDICINE;

EID: 34447518541     PISSN: 10802924     EISSN: None     Source Type: Journal    
DOI: 10.1532/LH96.07005     Document Type: Article

References (25)

1. Lasne F, Martin L, Crepin N, de Ceaurriz J. Detection of isoelectric profiles of erythropoietin in urine: differentiation of natural and administered recombinant hormones. Anal Biochem. 2002;311:119-126.
2. Schwenke D, Müller RK. Erythropoietin und Doping. Dtsch Z Sportmed. 2002; 53:25-26.
3. Beullens M, Delanghe JR, Bollen M. False-positive detection of recombinant human erythropoietin in urine following strenuous physical exercise. Blood. 2006;107:4711-4713.
4. Ashenden M, Varlet-Marie E, Lasne F, Audran M. The effects of microdose recombinant human erythropoietin regimens in athletes. Haematologica. 2006:91:1143-1144.
5. Audran M, Gareau R, Matecki S, et al. Effects of erythropoietin administration in training athletes and possible indirect detection in doping control. Med Sci Sports Exerc. 1999;31:639-645.
6. Magnani M, Corsi D, Bianchi M, et al. 1999. Monitoring erythopoietin abuse in athletes. Br J Haematol. 1999;106:2522-62.
7. Sharpe K, Hopkins W, Emslie KR, et al. Development of reference ranges in elite athletes for markers of altered erythropoiesis. Haematologica. 2002;87:1248-1257.
8. Parisotto R, Gore CJ, Emslie KR, et al. A novel method utilising markers of altered erythropoiesis for the detection of recombinant human erythropoietin abuse in athletes. Haematologica. 2000;85:564-572.
9. Parisotto R, Wu M, Ashenden MJ, et al. 2001. Detection of recombinant human erythropoietin abuse in athletes utilizing markers of altered erythopoiesis. Haematologica. 2001;86:128-137.
10. Ashenden MJ, Gore CJ, Parisotto R, Sharpe K, Hopkins WG, Hahn AG. Effect of altitude on second-generation blood tests to detect erythropoietin abuse by athletes. Haematologica. 2003;88:1053-1062.
11. Fischer I, Walter H. Aspartate aminotransferase (GOT) from young and old human erythrocytes. J Lab Clin Med. 1971;78:736-745.
12. Schmidt W, Böning D, Braumann KM. Red cell age effects on metabolism and oxygen affinity in humans. Respir Physiol. 1987;68:215-225.
13. Robinson Y, Cristancho E, Böning D. Intravascular haemolysis and mean red blood cell age in athletes. Med Sci Sports Exerc. 2006;38:480-483.
14. Böning D, Cristancho E, Serrato M, et al. 2004. Hemoglobin mass and peak oxygen uptake in untrained and trained female altitude residents. Int J Sports Med. 2004;25:561-568.
15. Böning D, Maassen N, Jochum F, et al. After-effects of a high altitude expedition on blood. Int J Sports Med. 1997;18:179-185.
16. Robinson Y, Cristancho E, Böning D. An optimized method for the assay of the mean red blood cell-age-related enzyme aspartate aminotransferase. Lab Hematol. 2004;10:144-146.
17. Hütler, M, Beneke R, Böning D. Determination of circulating hemoglobin mass and related quantities by using capillary blood. Med Sci Sports Exerc. 2000;32:1024-1027.
18. Burge CM, Skinner SL. Determination of hemoglobin mass and blood volume with CO: evaluation and application of a method. J Appl Physiol. 1995;79:623-631.
19. Schmidt W, Maassen N, Trost F, Böning D. Training induced effects on blood volume, erythrocyte turnover and haemoglobin oxygen binding properties. Eur J Appl Physiol. 1988;57:490-498.
20. Böning D, Rojas J, Serrato M, et al. Hemoglobin mass and peak oxygen uptake in untrained and trained residents of moderate altitude. Int J Sports Med. 2001;22:572-578.
21. Dehnert C, Hütler M, Liu Y, et al. Erythropoiesis and performance after two weeks of living high and training low in well trained triathletes. Int J Sports Med. 2002;23:561-566.
22. Schmidt W, Heinicke K, Rojas J, et al. Blood volume and hemoglobin mass in endurance athletes from moderate altitude. Med Sci Sports Exerc. 2002;34:1934-1940.
23. Robinson Y, Hostmann A, Matenov A, Ertel W, Oberholzer A. Erythropoiesis in multiply injured patients. J Trauma. 2006;61:1285-1291.
24. Thomas C, Thomas L. Anemia of chronic disease: pathophysiology and laboratory diagnosis. Lab Hematol. 2005;11:14-23.
25. Hampl H, Riedel E, Scigalla P, Stabell U, Wendel G. Erythropoiesis and erythrocyte age distribution in hemodialysis patients undergoing erythropoietin therapy. Blood Purif. 1990;8:117-125.