Convergent synthesis and in vivo inhibitory effect on fat accumulation of (-)-ternatin, a highly N-methylated cyclic peptide

Bioorganic and Medicinal Chemistry Letters

Volumn 17, Issue 16, 2007, Pages 4447-4449

  Shimokawa, Kenichiro ^a   Yamada, Kaoru ^a   Kita, Masaki ^a   Uemura, Daisuke ^a  

^a NAGOYA UNIVERSITY   (Japan)

Author keywords

( ) Ternatin; Cyclic peptide; Inhibitory effect on fat accumulation; Obesity; Synthesis

Indexed keywords

CYCLOPEPTIDE; TERNATIN; UNCLASSIFIED DRUG;

ADIPOCYTE; ANIMAL BEHAVIOR; ANIMAL EXPERIMENT; ARTICLE; BODY WEIGHT; CHEMICAL ANALYSIS; CONTROLLED STUDY; DIET; DRUG EFFECT; DRUG SYNTHESIS; IN VIVO STUDY; LIPID DIET; LIPID STORAGE; METHYLATION; MOUSE; NONHUMAN; OBESITY;

ADIPOSE TISSUE; ANIMALS; BODY WEIGHT; DIET; DIETARY FATS; LIVER; MICE; ORGAN SIZE; PEPTIDES, CYCLIC; PROTEIN CONFORMATION;

MURINAE; MUS;

EID: 34447335836     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2007.06.015     Document Type: Article

References (12)

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12. Animals and diets; 4-week-old male C57BL/6J mice were purchased from Charles River Japan Co. Ltd (Kanagawa, Japan). The diets, MF (standard), and high-fat diet (composed of 40% beef suet, 10% cornstarch, 9% glucose, 4% AIN-76™ with minerals, AIN-76™ with vitamins, and 36% casein) were purchased from Oriental Yeast Co., Ltd (Tokyo, Japan). The mice were individually housed under a controlled temperature (24 ± 3 °C) and a 12-h light-dark cycle. The mice had free access to water and standard rodent diet for 8 days before the experiment. The mice were then randomly divided into three groups for the study. (-)-Ternatin (1) (5 mg/kg/day) was administered by oral injection in group 3. Food was changed twice a week. Daily food intake and weekly body weight for each mouse were measured throughout the study. After the 5-week feeding period, mice were anesthetized with ether and killed by cardiac puncture. Blood samples were obtained using heparinized syringes. Livers and fat tissues were collected and immediately weighed.