An automated fluorescence-based method for continuous assay of PP2A activity

Methods in Molecular Biology

Volumn 365, Issue , 2007, Pages 61-69

  Wegner, Adam M ^a   McConnell, Jamie L ^a   Blakely, Randy D ^a   Wadzinski, Brian E ^a  

^a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

DiFMUP; immune complex; Phosphatase; phosphatase assay; PP2A

Indexed keywords

6,8 DIFLUORO 4 METHYLUMBELLIFERYL PHOSPHATE; 6,8-DIFLUORO-4-METHYLUMBELLIFERYL PHOSPHATE; DRUG DERIVATIVE; HYMECROMONE; PHOSPHATASE; UNCLASSIFIED DRUG;

ARTICLE; CHEMISTRY; FLUORESCENCE; METABOLISM; METHODOLOGY; MOLECULAR BIOLOGY; REPRODUCIBILITY;

FLUORESCENCE; HYMECROMONE; MOLECULAR BIOLOGY; PHOSPHORIC MONOESTER HYDROLASES; REPRODUCIBILITY OF RESULTS;

EID: 34447271534     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1385/1-59745-267-X:61     Document Type: Article

References (11)

1. Geladopoulos, T. P., Sotiroudis, T. G., and Evangelopoulos, A. E. (1991) A malachite green colorimetric assay for protein phosphatase activity. Anal. Biochem. 192, 112-116.
2. Sun, W. C., Gee, K. R., and Haugland, R. P. (1998) Synthesis of novel fluorinated coumarins: excellent UV-light excitable fluorescent dyes. Bioorg. Med. Chem. Lett. 8, 3107-3110. (Pubitemid 28551629)
3. Montalibet, J., Skorey, K.I., and Kennedy, B. P. (2005) Protein tyrosine phosphatase: enzymatic assays. Methods (Duluth) 35, 2-8. (Pubitemid 39647573)
4. Fontal, O. I., Vieytes, M. R., Baptista de Sousa, J. M., Louzao, M. C., and Botana, L. M. (1999) A fluorescent microplate assay for microcystin-LR. Anal. Biochem. 269, 289-296. (Pubitemid 29201026)
5. Kerby, M., and Chien, R.L. (2001) A fluorogenic assay using pressure-driven flow on a microchip. Electrophoresis. 22, 3916-3923. (Pubitemid 33040209)
6. Fjeld, C. C., Rice, A. E., Kim, Y., Gee, K. R., and Denu, J. M. (2000) Mechanistic basis for catalytic activation of mitogen-activated protein kinase phosphatase 3 by extracellular signal-regulated kinase. J. Biol. Chem. 275, 6749-6757. (Pubitemid 30146211)
7. Gee, K. R., Sun, W. C., Bhalgat, M. K., et al. (1999) Fluorogenic substrates based on fluorinated umbelliferones for continuous assays of phosphatases and betagalactosidases. Anal. Biochem. 273, 41-48. (Pubitemid 29400068)
8. Bauman, A. L., Apparsundaram, S., Ramamoorthy, S., Wadzinski, B. E., Vaughan, R. A., and Blakely, R. D. (2000) Cocaine and antidepressant-sensitive biogenic amine transporters exist in regulated complexes with protein phosphatase 2A. J. Neurosci. 20, 7571-7578.
9. Huang, Z., Wang, Q., Ly, H. D., et al. (1999) 3,6-Fluorescein diphosphate: a sensitive fluorogenic and chromogenic substrate for protein tyrosine phosphatases. J. Biomol. Screen. 4, 327-334. (Pubitemid 30106080)
10. Welte, S., Baringhaus, K.-H., Schmider, W., Muller, G., Petry, S., and Tennagels, N. (2005) 6,8-Difluoro-4-methylumbiliferyl phosphate: a fluorogenic substrate for protein tyrosine phosphatases. Anal. Biochem. 338, 32-38. (Pubitemid 40228895)
11. Favre, B., Zolnierowicz, S., Turowski, P., and Hemmings, B. A. (1994) The catalytic subunit of protein phosphatase 2A is carboxyl-methylated in vivo. J. Biol. Chem. 269, 16,311-16,317. (Pubitemid 24209328)