NAD-dependent isocitrate dehydrogenase mutants of Arabidopsis suggest the enzyme is not limiting for nitrogen assimilation

Plant Physiology

Volumn 144, Issue 3, 2007, Pages 1546-1558

  Lemaitre, Thomas ^a,c   Urbanczyk Wochniak, Ewa ^b,d   Flesch, Valerie ^a   Bismuth, Evelyne ^a   Fernie, Alisdair R ^b   Hodges, Michael ^a  

^a INSTITUTE OF PLANT SCIENCES PARIS SACLAY IPS2   (France)

^b MAX PLANCK INSTITUTE OF MOLECULAR PLANT PHYSIOLOGY   (Germany)

^c CEMAGREF   (France)

^d PLANT BIOLOGY DIVISION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYMES; FORESTRY; GAS CHROMATOGRAPHY; GENES; NITROGEN; ORGANIC ACIDS; PYRIDINE;

AMMONIUM; GENE PRODUCTS; METABOLIC PROFILING; SOIL-GROWN MUTANTS;

PLANTS (BOTANY);

ENZYMES; NITROGEN; ORGANIC ACIDS; PLANTS; PYRIDINE;

ARABIDOPSIS; ARABIDOPSIS THALIANA;

EID: 34447115721     PISSN: 00320889     EISSN: 15322548     Source Type: Journal    
DOI: 10.1104/pp.107.100677     Document Type: Article

References (58)

1. Abiko T, Obara M, Ushioda A, Hayakawa T, Hodges M, Yamaya T (2005) Localization of NAD-isocitrate dehydrogenase and glutamate dehydrogenase in rice roots: candidates for providing carbon skeletons to NADH-glutamate synthase. Plant Cell Physiol 46: 1724-1734
2. Blasing OE, Gibon Y, Gunther M, Hohne M, Morcuende R, Osuna D, Thimm O, Usadel B, Scheible WR, Stitt M (2005) Sugars and circadian regulation make major contributions to the global regulation of diurnal gene expression in Arabidopsis. Plant Cell 17: 3257-3281
3. Bologa KL, Fernie AR, Leisse A, Loureiro ME, Geigenberger P (2003) A bypass of sucrose synthase leads to low internal oxygen and impaired metabolic performance in growing potato tubers. Plant Physiol 132: 2058-2072
4. Bouchez D, Camilleri C, Caboche M (1993) A binary vector based on basta resistance in planta transformation of Arabidopsis thaliana. C R Acad Sci III Sci Vie 316: 1188-1198
5. Budde RJ, Randall DD (1990) Pea leaf mitochondrial pyruvate dehydrogenase complex is inactivated in vivo in a light-dependent manner. Proc Natl Acad Sci USA 87: 673-676
6. Bykova NV, Egsgaard H, Möller IM (2003) Identification of 14 new phosphoproteins involved in important plant mitochondrial processes. FEBS Lett 540: 141-146
7. Carrari F, Nunes-Nesi A, Gibon Y, Lytovchenko A, Loureiro ME, Fernie AR (2003) Reduced expression of aconitase results in an enhanced rate of photosynthesis and marked shifts in carbon partitioning in illuminated leaves of wild species tomato. Plant Physiol 133: 1322-1335
8. +)-dependent isocitrate dehydrogenases in mitochondria purified from Picea abies seedlings. Physiol Plant 96: 312-318
9. Chen R (1998) Plant NADP-dependent isocitrate dehydrogenases are predominantly localized in the cytosol. Planta 207: 280-285
10. Chen RD, Gadal P (1990) Do mitochondria provide the 2-oxoglutarate needed for synthesis in higher plant chloroplasts? Plant Physiol Biochem 28: 141-145
11. +)-dependent isocitrate dehydrogenase: cloning, nucleotide sequence, and disruption of the IDH2 gene from Saccharomyces cerevisiae. J Biol Chem 266: 22199-22205
12. +)-dependent isocitrate dehydrogenase from Saccharomyces cerevisiae. J BiolChem 267: 16417-16423
13. Fernie AR, Carrari F, Sweetlove LJ (2004a) Respiratory metabolism: glycolysis, the TCA cycle and mitochondrial electron transport. Curr Opin Plant Biol 7: 254-261
14. Fernie AR, Trethewey RN, Krotzky AJ, Willmitzer L (2004b) Metabolic profiling: from diagnostics to system biology. Nat Rev Mol Cell Biol 5: 763-769
15. Gálvez S, Bismuth E, Sarda C, Gadal P (1994) Purification and characterization of chloroplast NADP-isocitrate dehydrogenase from mixotrophic tobacco cells (comparison with the cytosolic enzyme). Plant Physiol 105: 593-600
16. Gálvez S, Lancien M, Hodges M (1999) Are isocitrate dehydrogenases and 2-oxoglutarate involved in the regulation of glutamate synthesis? Trends Plant Sci 4: 484-490
17. Geigenberger P (2003) Response of plant metabolism to too little oxygen. Curr Opin Plant Biol 6: 247-256
18. Hanning I, Baumgarten K, Schott K, Heldt HW (1999) Oxaloacetate transport into plant mitochondria. Plant Physiol 119: 1025-1032
19. Hodges M, Flesch V, Gálvez S, Bismuth E (2003) Higher plant NADP-dependent isocitrate dehydrogenases, ammonium assimilation and NADPH production. Plant Physiol Biochem 41: 577-585
20. Igamberdiev AU, Gardeström P (2003) Regulation of NAD- and NADP-dependent isocitrate dehydrogenases by reduction levels of pyridine nucleotides in mitochondria and cytosol of pea leaves. Biochim Biophys Acta 1606: 117-125
21. Jang JC, Sheen J (1994) Sugar sensing in higher plants. Plant Cell 6: 1665-1679
22. 2 dependence of quantum yield and respiration in Eucalyptus pauciflora. Plant Physiol 83: 1032-1036
23. Koch KE (1996) Carbohydrate-modulated gene expression in plants. Annu Rev Plant Physiol Plant Mol Biol 47: 509-540
24. Kruse A, Fieuw S, Heineke D, Müller-Röber B (1998) Antisense inhibition of cytosolic NADP-dependent isocitrate dehydrogenase in transgenic potato plants. Planta 205: 82-91
25. Laemmli UK (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227: 680-685
26. Lancien M, Ferrario-Méry S, Roux Y, Bismuth E, Macslaux C, Hirel B, Gadal P, Hodges M (1999) Simultaneous expression of NAD-dependent isocitrate dehydrogenase and other Krebs cycle genes after nitrate re-supply to short-term nitrogen-starved tobacco. Plant Physiol 120: 717-726
27. Lancien M, Gadal P, Hodges M (1998) Molecular characterization of higher plant NAD-dependent isocitrate dehydrogenase: evidence for a heteromeric structure by the complementation of yeast mutants. Plant J 16: 325-333
28. Landschütze V, Willmitzer L, Müller-Röber B (1995) Inhibition of flower formation by antisense repression of mitochondrial citrate synthase in transgenic potato plants leads to a specific disintegration of the ovary tissues of flowers. EMBO J 14: 660-666
29. + chez Arabidopsis thaliana. PhD thesis. Université de Paris Sud-XI, Orsay, France
30. Lemaitre T, Hodges M (2006) Expression analysis of Arabidopsis thaliana NAD-dependent isocitrate dehydrogenase genes shows the presence of a functional subunit that is mainly expressed in the pollen and absent from vegetative organs. Plant Cell Physiol 47: 634-643
31. +) and allosteric activator (AMP). J Biol Chem 278: 12864-12872
32. +-dependent isocitrate dehydrogenase from Arabidopsis thaliana. Plant Sci 166: 983-988
33. Matsuo M, Obokata J (2006) Remote control of photosynthetic genes by the mitochondrial respiratory chain. Plant J 47: 873-882
34. +)-dependent isocitrate dehydrogenase activity from etiolated pea mitochondria. Plant Sci 129: 9-20
35. Morcuende R, Krapp A, Hurry V, Stitt M (1998) Sucrose-feeding leads to increased rates of nitrate assimilation, increased rates of α-oxoglutarate synthesis, and increased synthesis of a wide spectrum of amino acids in tobacco leaves. Planta 206: 394-409
36. +)-isocitrate dehydrogenase and oxoglutarate dehydrogenase from the yeast Saccharomyces cervisiae and rat heart. Biochem J 303: 461-465
37. Nunes-Nesi A, Carrari F, Lytovchenko A, Smith AM, Loureiro ME, Ratcliffe RG, Sweetlove LJ, Fernie AR (2005) Enhanced photosynthetic performance and growth as a consequence of decreasing mitochondrial malate dehydrogenase activity in transgenic tomato plants. Plant Physiol 137: 611-622
38. Nunes-Nesi A, Sweetlove LJ, Fernie AR (2007) Operation and function of the tricarboxylic acid cycle in the illuminated leaf. Physiol Plant 129: 45-56
39. Parinov S, Sevugan M, Ye D, Yang WC, Kumaran M, Sundaresan V (1999) Analysis of flanking sequences from dissociation insertion lines: a database for reverse genetics in Arabidopsis. Plant Cell 11: 2263-2270
40. Queval G, Noctor G (2007) A plate reader method for the measurement of NAD, NADP, glutathione, and ascorbate in tissue extracts: application to redox profiling during Arabidopsis rosette development. Anal Biochem 363: 58-69
41. Raghavendra AS, Padmasree K (2003) Beneficial interactions of mitochondrial metabolism with photosynthetic carbon assimilation. Trends Plant Sci 8: 546-553
42. Rebeille F, Gans P, Chagvardieff P, Pean M, Tapie P, Thibault E (1988) Mass spectrometric determination of the inorganic carbon species assimilated by photoautotrophic cells of Europhorbia characias L. J Biol Chem 263: 12373-12377
43. Roessner U, Luedemann A, Brust D, Fiehn O, Linke T, Willmitzer L, Fernie AR (2001) Metabolic profiling allows comprehensive phenotyping of genetically or environmentally modified plant systems. Plant Cell 13: 11-29
44. Rosen H (1957) A modified ninhydrin colorimetric analysis for amino acids. Arch Biochem Biophys 67: 10-15
45. Scheibe R, Backhausen JE, Emmerlich V, Holtgrefe S (2005) Strategies to maintain redox homeostasis during photosynthesis under changing conditions. J Exp Bot 56: 1481-1489
46. Scheible WR, Morcuende R, Czechowski T, Fritz C, Osuna D, Palacios-Rojas N, Schindelasch D, Thimm O, Udvardi MK, Stitt M (2004) Genome-wide reprogramming of primary and secondary metabolism, protein synthesis, cellular growth processes and regulatory infrastructure of Arabidopsis in response to nitrogen. Plant Physiol 136: 2483-2499
47. Sessions A, Burke E, Presting G, Aux G, McElver J, Patton D, Dietrich B, Ho P, Bacwaden J, Ko C, et al (2002) A high-throughput Arabidopsis reverse genetics system. Plant Cell 14: 2985-2994
48. Smeekens S (2000) Sugar-induced signal transduction in plants. Annu Rev Plant Physiol Plant Mol Biol 51: 49-81
49. Sweetlove LJ, Lytovchenko A, Morgan M, Nunes-Nesi A, Taylor NL, Baxter CJ, Eickmeier I, Fernie AR (2006) Mitochondrial uncoupling protein is required for efficient photosynthesis. Proc Natl Acad Sci USA 103: 19587-19592
50. Tcherkez G, Cornic G, Bligny R, Gout E, Ghashghaie J (2005) In vivo respiratory metabolism of illuminated leaves. Plant Physiol 138: 1596-1606
51. Tovar-Méndez A, Miernyk JA, Randall DD (2003) Regulation of pyruvate dehydrogenase complex activity in plant cells. Eur J Biochem 270: 1043-1049
52. Towbin H, Staehlin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350-4354
53. Urbanczyk-Wochniak E, Baxter C, Kolbe A, Kopka J, Sweetlove LJ, Fernie AR (2005) Profiling of diurnal patterns of metabolite and transcript abundance in potato (Solanum tuberosum) leaves. Planta 221: 891-903
54. Wagner S, Bernhardt A, Leuendorf JE, Drewke C, Lytovchenko A, Mujahed N, Gurgui C, Frommer WB, Leistner E, Fernie AR, et al (2006) Analysis of the Arabidopsis rsr4-1/pdx1-3 mutant reveals the critical function of the PDX1 protein family in metabolism, development, and vitamin B6 biosynthesis. Plant Cell 18: 1722-1735
55. Weiss C, Zeng Y, Huang J, Sobocka MB, Rushbrook JI (2000) Bovine NAD+-dependent isocitrate dehydrogenase: alternative splicing and tissue-dependent expression of subunit 1. Biochemistry 39: 1807-1816
56. Wiskich JT, Dry IB (1985) The tricarboxylic acid cycle in plant mitochondria: its operation and regulation. In R Douce, DA Day, eds, Encyclopedia of Plant Physiology, Higher Plant Cell Respiration 18. Springer-Verlag, Berlin, pp 281-313
57. Zhao WN, McAlister-Henn L (1996) Expression and gene disruption analysis of the isocitrate dehydrogenase family in yeast. Biochemistry 35: 7873-7878
58. Zhao WN, McAlister-Henn L (1997) Affinity purification and kinetic analysis of mutant forms of yeast NAD(+)-specific isocitrate dehydrogenase. J Biol Chem 272: 21811-21817