N-acetyl-L-cysteine affords protection against lead-induced cytotoxicity and oxidative stress in human liver carcinoma (HepG2) cells

International Journal of Environmental Research and Public Health

Volumn 4, Issue 2, 2007, Pages 132-137

  Yedjou, Clement G ^a   Tchounwou, Paul B ^a  

^a Jackson State University   (United States)

Author keywords

Cytotoxicity; HepG2 cells; Lead nitrate; MDA; NAC; Oxidative stress

Indexed keywords

ACETYLCYSTEINE; LEAD; REACTIVE OXYGEN METABOLITE; THIOBARBITURIC ACID; TRYPAN BLUE;

CELL PROTECTION; CELL VIABILITY; CONFERENCE PAPER; CONTROLLED STUDY; CYTOTOXICITY; HUMAN; LIPID PEROXIDATION; LIVER CARCINOMA; OXIDATIVE STRESS; SALVAGE THERAPY; STATISTICAL SIGNIFICANCE;

EID: 34347370225     PISSN: None     EISSN: 16604601     Source Type: Journal    
DOI: 10.3390/ijerph2007040007     Document Type: Conference Paper

References (41)

1. ATSDR. The nature and extent of lead poisoning in the United States. A report to Congress. Atlanta, GA: Agency for Toxic Substances and Disease Registry, 1988.
2. U.S. Environmental Protection Agency (EPA). Evaluation of the Potential Carcinogenicity of Lead and Lead Compounds. Office of Health and Environmental Assessment. EPA/600/8-89/045A, 1989.
3. Cory-Slechta, D. A.; Pound, J. G.: Lead neurotoxicity. In Handbook of Neurotoxicology (L.W. Chang and R.S. Dyer, Eds.) Dekker, New York, 1995, pp. 61-89.
4. Goyer, R. A.: Lead toxicity: current concerns. Environ Health Perspect. 1993, 100: 177-187.
5. Bechara, E. J. H.; Medeiros, M. H. G.; Monteiro, H. P.; Her-mes-Lim, M.; Pereira, B.; Demasi, M.; Costa, C. A.; Ab-dalla, D. S. P.; Onuk, J.; Wendel, C. M. A.; Di Mascio, P.: A free-radical hypothesis of lead poisoning and inborn porphyrias associated with 5-aminolevulinic acid overload. Quim. Nova, 1993, 16: 385-92.
6. Hermes-Lima, M.; Pereira, B.; Bechara, E. J. H.: Are free radicals involved in lead poisoning? Xenobiotica 1991, 21: 1085-1090.
7. Jiun, Y. S.; Hsien, L. T.: Lipid peroxidation in workers exposed to lead. Archiv. Environ. Health 1994, 49: 256-259.
8. Monteiro, H. P.; Abdalla, D. S. P.; Arcuri, A. S.; Bechara, E. J. H.: Oxygen toxicity related to exposure to lead. Clin. Chem. 1985, 31: 1673-1676.
9. Gurer, H.; Ozgunes, H.; Oztezcan, S.; Ercal, N.: Antioxidant role of α-lipoic cid in lead toxicity. Free Radic. Biol. Med. 1999, 27: 75-81.
10. Gurer, H.; Ercal, N.: Can antioxidants be beneficial in the treatment of lead poisoning? Free Radic. Biol. Med. 2000, 29: 927-945.
11. Baas, P.; Oppelaar, H.; van der Valk, M. A.; van Zandwijk, N.; Stewart, F. A.: Partial protection of photodynamic-induced skin reactions in mice by N-acetylcysteine: a preclinical study. Photochem Photobiol, 1994, 59: 448-454.
12. Emonet-Piccardi, N.; Richard, M. J.; Ravanat, J. L.; Signorini, N.; Cadet, J.; Beani, J. C.: Protective effects of antioxidants against UVA-induced DNA damage in human skin fibroblasts in culture. Free Radic. Res. 1998, 29: 307-313.
13. De Flora, S.; Izzotti, A.; D'Agostini, F.; Balansky, R. M.: Mechanisms of N-acetylcysteine in the prevention of DNA damage and cancer, with special reference to smoking-related end-points. Carcinogenesis. 2001, 22: 999-1013.
14. De Vries, N.; De Flora, S.: N-acetyl-L-cysteine. J. Cell. Biochem, 1993, 17: 270-277.
15. Shan, X. Q.; Aw, T. Y.; Jones, D. P.: Glutathione-dependent protection against oxidative injury. Pharmacol Ther , 1990, 47: 61-71.
16. Thomas, S. H.: Paracetamol (acetaminophen) poisoning. Pharmacol Ther, 1993, 60: 91-120.
17. De Flora S.; Cesarone, C. F., Balansky, R. M.; Albini, A., D'Agostini, F.; Bennicelli, C.: Chemopreventive properties and mechanisms of N-acetylcysteine: the experimental background. J. Cell. Biochem., 1995, 22: 33-41.
18. Pendyala, L.; Creaven, P. J.: Pharmacokinetic and pharmacodynamic studies of N-acetylcysteine, a potential chemopreventive agent during phase 1 trial. Cancer Epidemiol. Biomarkers Prev., 1995, 4: 245-251.
19. Guo, B. Y.; Cheng, Q. K.: Chelating capability of tea components with metal ion. J. Tea Science, 1991, 11: 139-144.
20. Kumamoto, M.; Sonda, T.; Nagayama, K.; Tabata, M.: Effects of pH and metal ions on antioxidative activities of catechins. Biosci. Biotechnol. Biochem. 2001, 65: 126-132.
21. Borenfreund, E.; Babich, H.; Martin-Alguacil, N.: Rapid chemosensitivity assay with human normal and tumor cells in vitro. In Vitro Cell Dev. Biology, 1990, 26: 1030-1034.
22. Marinovitch, M.; Lorenzo, J.; Flaminio, L.; Ganata, A.; Galli, C.: The HepG2 cell line as a possible alternative to isolated hepatocytes in cytotoxicity studies. ATLA. 1988, 16: 16-22.
23. Mosmann, T.: Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.J. Immunol. Methods, 1983, 65: 55-63.
24. Tchounwou, P. B.; Wilson, B.; Schneider, J.; Ishaque, A.: Cytogenic assessment of arsenic trioxide toxicity in the Mutatox, Ames II and CAT-Tox assays. Metal Ions Biol. Med, 2000, 6: 89-91.
25. Tchounwou, P. B.; Yedjou, C. G.; Foxx, D.; Ishaque, A.; Shen, E.: Lead induced cytotoxicity and transcriptional activation of stress genes in human liver carcinoma cells. Mol. Cell. Biochem, 2004, 255: 161-170.
26. 2) cells. Metal Ions Biol. Med., 2006, 9: 293-297.
27. 2) cells exposed to arsenic trioxide and monosodium acid methanearsonate (MSMA). Int J Mol. Sci., 2002, 3:1117-1132.
28. TM, 2003, 49( 7): 1071-1079.
29. Yedjou, C. G.; Moore, P.; Tchounwou, P. B.: Dose and Time-Dependent Response of Human Acute Promyelocytic Leukemia HL-60 Cells to Arsenic Trioxide Treatment. Int. J. Environ. Res. Public Health, 2006, 3(2): 136-140.
30. Cai, T.; Fassina, G., Morini, M.; Aluigi, M. G.; Masiello, L., Fontanini, G., D'Agostini, F.; De Flora, S.; Noonan, D. M.; Albini A.: N-acetylcysteine inhibits endothelial cell invasion and angiogenesis. Lab Invest, 1999, 79: 1151-1159.
31. Moldeus, P.; Berggren, M.; Grafstrom, R.: N-acetylcysteine protection against the toxicity of cigarette smoke and cigarette smoke condensates in various tissues and cells in vitro. Eur. J. Resp. Dis. 1985, 39 (suppl), 123-129.
32. Aluigi, M. G.; De Flora, S.; D'Agostini, F.; Albini, A.; Fassina, G.: Antiapoptotic and antigenotoxic effects of N-acetylcysteine in human cells of endothelial origin. Anticancer Res., 2000, 20: 3183-3187.
33. Halliwell, B.; Gutteridge, J. M. C.: Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem. J, 1984, 219: 1-14.
34. Ding, Y.; Gonick, H. C.; Vaziri, N. D.: Lead promotes hydroxyl radical generation and lipid peroxidation in cultured aortic endothelial cells. Am J Hypertens., 2000, 13: 552-555.
35. Adonaylo, V. N.; Oteiza, P. I.: Lead intoxication: Antioxidant defenses and oxidative damage in rat brain.Toxicology. 1999, 135: 77-85.
36. Sandhir, R.; Gill, K. D.: Effect of lead on lipid peroxidation in liver of rats. Biol. Trace Elem. Res. 1995, 48: 91-97.
37. Yedjou, C. G.; Tchounwou, P. B.: Oxidative stress in human leukemia (HL-60), human liver carcinoma (HepG2), and human jurkat T-cells exposed to arsenic trioxide. Metal Ions Biol. Med., 2006, 9: 298-303.
38. Cotgreave, I. A.: N-acetylcysteine: Pharmacological considerations and experimental and clinical applications. Adv. Pharmacol. 1997, 38: 205-22.
39. Mayer, M.; Noble, M.: N-Acetyl-L-cysteine is a pluripotent protector against cell death and enhancer of trophic factor-mediated cell survival in vitro. Proc. Natl. Acad. Sci. U.S.A. 1994, 91: 7496-7500.
40. Suttorp, N.; Kastle, S.; Neuhof, H.: Glutathione redox cycle is important defense system of endothelial cells against chronic hyperoxia. Lung. 1991, 169: 203-214.
41. Junod, A. F.; Jurnot, L.; Grichting, G.: Comparative study on selenium-and-n-aceltylcysteine-related effects on the toxic action of hyperoxia, paraquat and enzyme reaction hypoxanthine-xanthine oxidative in culture endothelial cells. Agents Actions, 1987, 22: 176-183.