Protective effects of antioxidants against UVA-induced DNA damage in human skin fibroblasts in culture

Free Radical Research

Volumn 29, Issue 4, 1998, Pages 307-313

  Emonet Piccardi, N ^a   Richard, M J ^a,c   Ravanat, J L ^b   Signorini, N ^b   Cadet, J ^b   Beani J C ^a  

^a UNIV GRENOBLE ALPES   (France)

^b CEA GRENOBLE   (France)

^c HÔPITAL MICHALLON   (France)

Author keywords

Comet assay; Human skin fibroblasts; N acetyl cysteine; Strand breaks; Trace elements; UVA radiation

Indexed keywords

ACETYLCYSTEINE; AGAROSE; ANTIOXIDANT; MUTAGENIC AGENT; PROTECTIVE AGENT; SELENIUM; SODIUM SELENITE; ZINC; ZINC CHLORIDE;

ARTICLE; ASSAY; CELL CULTURE; CONTROLLED STUDY; CYTOTOXICITY; DNA DAMAGE; GEL ELECTROPHORESIS; HUMAN; HUMAN CELL; SKIN FIBROBLAST; ULTRAVIOLET A RADIATION;

ACETYLCYSTEINE; ADULT; ANTIOXIDANTS; BREAST; CELLS, CULTURED; CHLORIDES; DNA DAMAGE; ELECTROPHORESIS, AGAR GEL; FEMALE; FIBROBLASTS; HUMANS; SKIN; SODIUM SELENITE; ULTRAVIOLET RAYS; ZINC COMPOUNDS;

EID: 0032428720     PISSN: 10715762     EISSN: None     Source Type: Journal    
DOI: 10.1080/10715769800300341     Document Type: Article

References (37)

1. J. Cadet, M. Berger, T. Douki, B. Morin, S. Raoul, J.-L. Ravanat and S. Spinelli (1997) Effects of UV and visible radiation on DNA-final base damage. Biological Chemistry, 378, 1275-1286.
2. J. Cadet, M. Berger, T. Douki and J.-L. Ravanat (1997) Oxidative damage to DNA. Formation, measurement and biological significance. Reviews of Physiology, Biochemistry and Pharmacology, 131, 1-87.
3. S. Basu Modak and R.M. Tyrrell (1993) Singlet oxygen: a primary effector in the ultraviolet A/near visible light induction of the human heme oxygenase gene. Cancer Research, 53, 4505-4510.
4. A. Fischer-Nielsen, S. Loft and K.G. Jensen (1993) Effect of ascorbate and 5-aminosalicylic acid on light induced 8-hydroxydeoxyguanosine formation in V79 Chinese hamster cells. Carcinogenesis, 14, 2431-2433.
5. 2 alone or with UVB or UVA irradiation. Photochemistry and Pholobiology, 64, 117-122.
6. R.M. Tyrrell (1996) Oxidant, antioxidant status and photocarcinogenesis: the role of gene activation. Photochemistry and Photobiology, 63, 380-383.
7. W.G. Warmer and R.R. Wei (1997) In vitro photooxidation of nucleic acids by ultraviolet A radiation. Photochemistry and Photobiology, 65, 560-563.
8. X. Zhang, B.S. Rosenstein, Y. Wang, M. Lebwohl, D.M. Mitchell and H. Wei (1997) Induction of 8-oxo-7,8-dihydro-2′-deoxyguanosine by ultraviolet radiation in calf thymus DNA and HeLa cells. Photochemistry and Photobiology, 65, 119-124.
9. N. Emonet, M.-T. Leccia, A. Favier, J.-C. Béani and M.-J. Richard (1997) Thiols and selenium: protective effect on human skin fibroblasts exposed to ultraviolet A radiation. Journal of Photochemistry and Photobiology, B: Biology, 40, 84-90.
10. M.-T. Leccia, M.-J. Richard, J.-C. Béani, H. Faure, A.-M. Monjo, J. Cadet, P. Amblard and A. Favier (1993) Protective effect of selenium and zinc on UVA damage in human skin fibroblasts. Photochemistry and Photobiology, 58, 548-553.
11. V.N. Gladyshev, K.T. Jeang and T.C. Stadtman (1996) Selenocysteine, identified as the penultimate C-terminal residue in human T-cell thioredoxin reductase, corresponds to TGA in the human placental gene. Proceedings of the National Academy of Sciences of the United States of America, 93, 6146-6151.
12. R.L. Willson (1989) Zinc and iron in free radical pathology and cellular control. In Zinc in Human Biology (ed. C.F. Mills) Springer-Verlag, London, pp. 147-172.
13. J.F. Coleman (1992) Zinc proteins: enzymes, storage proteins, transcription factors, and replication proteins. Annual Review of Biochemistry, 61, 897-946.
14. E.J. Dudek, J.G. Peak, R.M. Roth and M.J. Peak (1990) Protection against UV-A induced photooxidative damage in mammalian cell lines expressing increased levels of metallothionein. Free Radical Biology and Medicine, 9, 336-340.
15. L.S. Chubatsu and R. Meneghini (1993) Metallothionein protects DNA from oxidative damage. Biochemical Journal, 291, 193-198.
16. M.-O. Parat, M.-J. Richard, S. Pollet, C. Hadjur, A. Favier and J.-C. Béani (1997) Zinc and DNA fragmentation in keratinocyte apoptosis: its inhibitory effect in UVB irradiated cells. Journal of Photochemistry and Photobiology, B: Biology, 37, 101-106.
17. H.C. Birnboim and J.J. Jevcak (1981) Fluorimetric method for rapid detection of DNA strand breaks in human white blood cells produced by low doses of radiation. Cancer Research, 41, 1889-1892.
18. D. Anderson, T.-W. Yu, B.J. Phillips and P. Schezer (1994) The effect of various antioxidants and modifying agents on oxygen-radical-generated DNA damage in human lymphocytes in the Comet assay. Mutation Research, 307, 261-271.
19. A.R. Collins, M. Al-Guo and S.J. Duthie (1995) The kinetics of repair of oxidative DNA damage (strand breaks and oxidized pyrimidines) in human cells. Mutation Research, 336, 69-77.
20. A.R. Collins, M. Dusinska, C.M. Gedik and R. Stetina (1996) Oxidative damage to DNA: Do we have a reliable biomarker? Environmental Health Perspectives, 104, 465-469.
21. C. Alapetite, T. Watcher, E. Sage and E. Moustacchi (1996) Use of the alkaline comet assay to detect DNA repair deficiencies in human fibroblasts exposed to UVC, UVB, UVA and γ-rays. International Journal of Radiation Biology, 69, 359-369.
22. O. Östling and K.J. Johanson (1984) Microelectrophoretic study of radiation-induced DNA damage in individual mammalian cells. Biochemical and Biophysical Research Communications, 123, 291-298.
23. N.P. Singh, M.T. McCoy, R.R. Tice and E.L. Schneider (1988) A simple technique for the quantification of low levels of DNA damage in individual cells. Experimental Cell Research, 175, 184-191.
24. B. Hellman, H. Vaghef and B. Boström (1995) The concepts of the tail moment and tail inertia in the single cell gel electrophoresis assay. Mutation Research, 336, 123-131.
25. J. Cadet, X. Artignan, M. Berger, T. Douki, M. Gromova, M. Polverelli, J.L. Ravanat and M.-J. Richard (1997) Effects of ionising radiation on isolated and cellular DNA. Radioprotection, 32-C1, 77-80.
26. M.J. Peak and J.G. Peak (1990) Hydroxyl radical quenching agents protect against DNA breakage caused by both 365 nm and by gamma radiation. Photochemistry and Photobiology, 51, 649-652.
27. R.M. Tyrrell and M. Pidoux (1988) Correlation between endogenous glutathione content and sensitivity of cultured human skin cells to radiation at defined wavelengths in solar ultraviolet range. Photochemistry and Photobiology, 47, 405-412.
28. L.A. Applegate, D. Lautier, E. Frenk and R.M. Tyrrell (1992) Endogenous glutathione levels modulate the frequency of both spontaneous and long wavelength ultraviolet induced mutations in human cells. Carcinogenesis, 13, 1557-1560.
29. H.J. Niggli and L.A. Applegate (1997) Glutathione response after UVA irradiation in mitotic and postmitotic human skin fibroblasts and keratinocytes. Photochemistry and Photobiology, 65, 680-684.
30. J.H.R. Kagi and Y. Kojima (1987) Chemistry and biochemistry of metallothionein. In Experientia, Metallothionein II (eds. J.H.R. Kagi and Y. Kojima), Birkhäuser Verlag, Basel, suppl. 52, pp. 25-61.
31. A.J. McGowan, R.S. Fernandes, S. Verhaegen and T.G. Cotter (1994) Zinc inhibits UV radiation induced apoptosis but fails to prevent subsequent cell death. International Journal of Radiation Biology, 66, 343-349.
32. E.J. Dudek, J.G. Peak, R.M. Roth and M.J. Peak (1993) Isolation of V79 fibroblast cell lines containing elevated metallothionein levels that have increased resistance to the cytotoxic effects of ultraviolet-A radiation. Photochemistry and Photobiology, 58, 836-840.
33. A. Bakka, A.S. Johnsen and H.E. Rugstad (1982) Radioresistance in cells with high content of metallothionein. Experientia, 38, 381-383.
34. P.J. Thornalley and M. Vasak (1985) Possible role for metallothionein in protection against radiation induced oxidative stress. Kinetics and mechanism of its interaction with superoxide and hydrogen radicals. Biochimica et Biophysica Acta, 827, 36-44.
35. A. Favier (1995) Zinc-ligand interactions and oxygen free radical formation. In Handbook of Metal-Ligand Interaction in Biological Fluids. (ed. G. Berthon), M. Dekker, New York, vol. 2, pp. 876-887.
36. M.-O. Parat, M.-J. Richard, J.-C. Béani and A. Favier (1997) Involvement of zinc in intracellular oxidant/antioxidant balance. Biological Trace Element Research, 60, 187-204.
37. M. Leist, B. Raab, S. Maurer, U. Rösick and R. Brigelius-Flohé (1996) Conventional cell culture media do not adequatly supply cells with antioxidants and thus facilitate peroxide-induced genotoxicity. Free Radical Biology and Medicine, 3, 297-306.